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Science 25 October 1996:
Vol. 274. no. 5287, pp. 610 - 614
DOI: 10.1126/science.274.5287.610
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Reports

Accessing Genetic Information with High-Density DNA Arrays

Mark Chee, Robert Yang, Earl Hubbell, Anthony Berno, Xiaohua C. Huang, David Stern, Jim Winkler, David J. Lockhart, Macdonald S. Morris, Stephen P. A. Fodor

Rapid access to genetic information is central to the revolution taking place in molecular genetics. The simultaneous analysis of the entire human mitochondrial genome is described here. DNA arrays containing up to 135,000 probes complementary to the 16.6-kilobase human mitochondrial genome were generated by light-directed chemical synthesis. A two-color labeling scheme was developed that allows simultaneous comparison of a polymorphic target to a reference DNA or RNA. Complete hybridization patterns were revealed in a matter of minutes. Sequence polymorphisms were detected with single-base resolution and unprecedented efficiency. The methods described are generic and can be used to address a variety of questions in molecular genetics including gene expression, genetic linkage, and genetic variability.

Affymetrix, 3380 Central Expressway, Santa Clara, CA 95051, USA.

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L. Beaudet, J. Bédard, B. Breton, R. J. Mercuri, and M. L. Budarf (2001)
Genome Res. 11, 600-608
   Abstract »    Full Text »
Mapping Maize Sequences to Chromosomes Using Oat-Maize Chromosome Addition Materials.
R. J. Okagaki, R. G. Kynast, S. M. Livingston, C. D. Russell, H. W. Rines, and R. L. Phillips (2001)
Plant Physiology 125, 1228-1235
   Abstract »    Full Text »
Portable System for Microbial Sample Preparation and Oligonucleotide Microarray Analysis.
S. G. Bavykin, J. P. Akowski, V. M. Zakhariev, V. E. Barsky, A. N. Perov, and A. D. Mirzabekov (2001)
Appl. Envir. Microbiol. 67, 922-928
   Abstract »    Full Text »
Enabling Large-Scale Pharmacogenetic Studies by High-Throughput Mutation Detection and Genotyping Technologies.
M. M. Shi (2001)
Clin. Chem. 47, 164-172
   Abstract »    Full Text »    PDF »
NTDB: Thermodynamic Database for Nucleic Acids.
W. L. A. K. Chiu, C. N. Sze, L. N. Ip, S. K. Chan, and S. C. F. Au-Yeung (2001)
Nucleic Acids Res. 29, 230-233
   Abstract »    Full Text »    PDF »
Gene Expression Changes Triggered by Exposure of Haemophilus influenzae to Novobiocin or Ciprofloxacin: Combined Transcription and Translation Analysis.
H. Gmuender, K. Kuratli, K. Di Padova, C. P. Gray, W. Keck, and S. Evers (2001)
Genome Res. 11, 28-42
   Abstract »    Full Text »
Transition characteristics and thermodynamic analysis of DNA duplex formation: a quantitative consideration for the extent of duplex association.
P. Wu and N. Sugimoto (2000)
Nucleic Acids Res. 28, 4762-4768
   Abstract »    Full Text »    PDF »
Toxicogenomics-Based Discrimination of Toxic Mechanism in HepG2 Human Hepatoma Cells.
M. E. Burczynski, M. McMillian, J. Ciervo, L. Li, J. B. Parker, R. T. Dunn II, S. Hicken, S. Farr, and M. D. Johnson (2000)
Toxicol. Sci. 58, 399-415
   Abstract »    Full Text »    PDF »


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