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Science 11 October 1996:
Vol. 274. no. 5285, pp. 242 - 246
DOI: 10.1126/science.274.5285.242
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Reports

Association of Spindle Assembly Checkpoint Component XMAD2 with Unattached Kinetochores

Rey-Huei Chen, Jennifer C. Waters, E. D. Salmon, Andrew W. Murray *

The spindle assembly checkpoint delays anaphase until all chromosomes are attached to a mitotic spindle. The mad (mitotic arrest-deficient) and bub (budding uninhibited by benzimidazole) mutants of budding yeast lack this checkpoint and fail to arrest the cell cycle when microtubules are depolymerized. A frog homolog of MAD2 (XMAD2) was isolated and found to play an essential role in the spindle assembly checkpoint in frog egg extracts. XMAD2 protein associated with unattached kinetochores in prometaphase and in nocodazole-treated cells and disappeared from kinetochores at metaphase in untreated cells, suggesting that XMAD2 plays a role in the activation of the checkpoint by unattached kinetochores. This study furthers understanding of the mechanism of cell cycle checkpoints in metazoa and provides a marker for studying the role of the spindle assembly checkpoint in the genetic instability of tumors.

R.-H. Chen and A. W. Murray, Department of Physiology, University of California, San Francisco, San Francisco, CA 94143, USA.
J. C. Waters and E. D. Salmon, Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA.
*   To whom correspondence should be addressed.

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Lesions in Many Different Spindle Components Activate the Spindle Checkpoint in the Budding Yeast Saccharomyces cerevisiae.
K. G. Hardwick, R. Li, C. Mistrot, R.-H. Chen, P. Dann, A. Rudner, and A. W. Murray (1999)
Genetics 152, 509-518
   Abstract »    Full Text »
Budding Yeast Bub2 Is Localized at Spindle Pole Bodies and Activates the Mitotic Checkpoint via a Different Pathway from Mad2.
R. Fraschini, E. Formenti, G. Lucchini, and S. Piatti (1999)
J. Cell Biol. 145, 979-991
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The Maize Homologue of the Cell Cycle Checkpoint Protein MAD2 Reveals Kinetochore Substructure and Contrasting Mitotic and Meiotic Localization Patterns.
H.-G. Yu, M. G. Muszynski, and R. Kelly Dawe (1999)
J. Cell Biol. 145, 425-435
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Science. ISSN 0036-8075 (print), 1095-9203 (online)