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Science 30 August 1996:
Vol. 273. no. 5279, pp. 1234 - 1236
DOI: 10.1126/science.273.5279.1234
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Reports

Induction of Gene Expression in Escherichia coli After Pilus-Mediated Adherence

Jian Ping Zhang * and Staffan Normark

The induction of cascades of virulence factors after contact between bacteria and host cells was investigated. P-pili mediate the binding of uropathogenic Escherichia coli to its host cell receptor. After P-pili binding there was transcriptional activation of a sensor-regulator protein that is essential for the bacterial iron-starvation response. An insertion mutation of the sensor-regulator gene eliminated the ability of uropathogenic E. coli to produce siderophores and their iron-regulated membrane receptors, thereby abolishing their ability to grow in urine. These results suggest that P-pilus-mediated attachment may be an important part of the sensor-regulatory process involved in uropathogenic E. coli urinary tract infection.

J. P. Zhang, Department of Molecular Microbiology, Washington University School of Medicine, 666 South Euclid Avenue, St. Louis, MO 63110-1093, USA.
S. Normark, Department of Molecular Microbiology, Washington University School of Medicine, 666 South Euclid Avenue, St. Louis, MO 63110-1093, USA, and Microbiology and Tumorbiology Center, Karolinska Institute, Box 1041, and Swedish Institute for Infectious Disease Control, S10521 Stockholm, Sweden.
*   To whom correspondence should be addressed at Microcide Pharmaceutical, Inc., 850 Maude Avenue, Mountain View, CA 94043, USA.


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