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Science 16 August 1996:
Vol. 273. no. 5277, pp. 951 - 953
DOI: 10.1126/science.273.5277.951
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Reports

Exclusion of Int-6 from PML Nuclear Bodies by Binding to the HTLV-I Tax Oncoprotein

Christelle Desbois, Raphaël Rousset, Frédéric Bantignies, Pierre Jalinot *

The Tax transactivator of the human T cell leukemia virus type I (HTLV-I) exhibits oncogenic properties. A screen for proteins interacting with Tax yielded a complementary DNA (cDNA) encoding the human Int-6 protein. In mice, the Int-6 gene can be converted into a putative dominant negative oncogene after retroviral insertion. Here, Int-6 was localized in the cell nucleus to give a speckled staining pattern superposed to that of the promyelocytic leukemia (PML) protein. The binding of Tax to Int-6 caused its redistribution from the nuclear domains to the cytoplasm. Thus, Int-6 is a component of the PML nuclear bodies and Tax disrupts its normal cellular localization by binding to it.

Laboratoire de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique UMR49, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, 69364 Lyon Cedex 07, France.
*   To whom correspondence should be addressed. E-mail: pjalinot{at}ens-lyon.fr


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