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Science 9 August 1996:
Vol. 273. no. 5276, pp. 784 - 788
DOI: 10.1126/science.273.5276.784
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Reports

Visualization of Slow Axonal Transport in Vivo

Sumio Terada, Takao Nakata, Alan C. Peterson, Nobutaka Hirokawa *

In axons, cytoskeletal constituents move by slow transport. However, it remains controversial whether axonal neurofilaments are dynamic structures in which only subunits are transported or whether filaments assemble in the proximal axon and are transported intact as polymers to the axon terminus. To investigate the form neurofilament proteins take during transport, neurons of transgenic mice lacking axonal neurofilaments were infected with a recombinant adenoviral vector encoding epitope-tagged neurofilament M. Confocal and electron microscopy revealed that the virally encoded neurofilament M was transported in unpolymerized form along axonal microtubules. Thus, neurofilament proteins are probably transported as subunits or small oligomers along microtubules, which are major routes for slow axonal transport.

S. Terada, Institute for Brain Research, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan.
T. Nakata, Department of Anatomy and Cell Biology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan.
A. C. Peterson, Molecular Oncology Group, McGill University, H5-35, 687 Pine Avenue West, Montreal, Quebec H3A 1A1, Canada.
N. Hirokawa, Institute for Brain Research and Department of Anatomy and Cell Biology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan.
*   To whom correspondence should be addressed. E-mail: hirokawa{at}m.u-tokyo.ac.jp


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