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Science 26 July 1996:
Vol. 273. no. 5274, pp. 513 - 516
DOI: 10.1126/science.273.5274.513
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Reports

Persistent Site-Specific Remodeling of a Nucleosome Array by Transient Action of the SWI/SNF Complex

T. Owen-Hughes, R. T. Utley, J. Côté, C. L. Peterson, J. L. Workman *

The SWI/SNF complex participates in the restructuring of chromatin for transcription. The function of the yeast SWI/SNF complex in the remodeling of a nucleosome array has now been analyzed in vitro. Binding of the purified SWI/SNF complex to a nucleosome array disrupted multiple nucleosomes in an adenosine triphosphate-dependent reaction. However, removal of SWI/SNF left a deoxyribonuclease I-hypersensitive site specifically at a nucleosome that was bound by derivatives of the transcription factor Gal4p. Analysis of individual nucleosomes revealed that the SWI/SNF complex catalyzed eviction of histones from the Gal4-bound nucleosomes. Thus, the transient action of the SWI/SNF complex facilitated irreversible disruption of transcription factor-bound nucleosomes.

T. Owen-Hughes, R. T. Utley, J. Côté, J. L. Workman, Department of Biochemistry and Molecular Biology and Center for Gene Regulation, Pennsylvania State University, University Park, PA 16802-4500, USA.
C. L. Peterson, Program in Molecular Medicine and Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, Worcester, MA 01605, USA.
*   To whom correspondence should be addressed. E-mail: JLW10{at}psu.edu


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Chemically Regulated Transcription Factors Reveal the Persistence of Repressor-resistant Transcription after Disrupting Activator Function.
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