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Science 19 July 1996:
Vol. 273. no. 5273, pp. 361 - 364
DOI: 10.1126/science.273.5273.361
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Reports

Interaction Between a Putative Mechanosensory Membrane Channel and a Collagen

Jingdong Liu, * Bertold Schrank, dagger Robert H. Waterston

The degenerin family of proteins in Caenorhabditis elegans is homologous to subunits of the mammalian amiloride-sensitive epithelial sodium channels. Mutations in nematode degenerins cause cell death, probably because of defects in channel function. Genetic evidence was obtained that the unc-105 gene product represents a degenerin homolog affecting C. elegans muscles and that this putative channel interacts with type IV collagen in the extracellular matrix underlying the muscle cell. This interaction may serve as a mechanism of stretch-activated muscle contraction, and this system could provide a molecular model for the activation of mechanosensitive ion channels.

Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA.
* To whom correspondence should be addressed. E-mail: liuj@genetics.wustl.edu
dagger Present address: Neurologische Universitätsklinik und Poliklinik im Kopfklinikum, Josef-Schneider-Strasse 11, 97080 Würzburg, Germany.


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