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Science 12 July 1996:
Vol. 273. no. 5272, pp. 242 - 245
DOI: 10.1126/science.273.5272.242
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Reports

Long-Term Lymphohematopoietic Reconstitution by a Single CD34-Low/Negative Hematopoietic Stem Cell

Masatake Osawa, * Ken-ichi Hanada, Hirofumi Hamada, Hiromitsu Nakauchi dagger

Hematopoietic stem cells (HSCs) supply all blood cells throughout life by making use of their self-renewal and multilineage differentiation capabilities. A monoclonal antibody raised to the mouse homolog of CD34 (mCD34) was used to purify mouse HSCs to near homogeneity. Unlike in humans, primitive adult mouse bone marrow HSCs were detected in the mCD34 low to negative fraction. Injection of a single mCD34lo/-, c-Kit+, Sca-1+, lineage markers negative (Lin-) cell resulted in long-term reconstitution of the lymphohematopoietic system in 21 percent of recipients. Thus, the purified HSC population should enable analysis of the self-renewal and multilineage differentiation of individual HSCs.

M. Osawa and H. Nakauchi, Department of Immunology, Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba Science-City, Ibaraki 305, Japan.
K.-i. Hanada and H. Hamada, Department of Molecular Biotherapy, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Tokyo 170, Japan.
* Present address: KIRIN Pharmaceutical Research Laboratory, Gunma 371, Japan.
dagger To whom correspondence should be addressed.


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Stem Cells 24, 653-661
   Abstract »    Full Text »    PDF »
Differential mRNA Processing in Hematopoietic Stem Cells.
T. V. Bowman, A. J. McCooey, A. A. Merchant, C. A. Ramos, P. Fonseca, A. Poindexter, S. B. Bradfute, D. M. Oliveira, R. Green, Y. Zheng, et al. (2006)
Stem Cells 24, 662-670
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Survival of the fittest: in vivo selection and stem cell gene therapy.
T. Neff, B. C. Beard, and H.-P. Kiem (2006)
Blood 107, 1751-1760
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Combinatorial Gata2 and Sca1 expression defines hematopoietic stem cells in the bone marrow niche.
N. Suzuki, O. Ohneda, N. Minegishi, M. Nishikawa, T. Ohta, S. Takahashi, J. D. Engel, and M. Yamamoto (2006)
PNAS 103, 2202-2207
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SLAM family markers are conserved among hematopoietic stem cells from old and reconstituted mice and markedly increase their purity.
O. H. Yilmaz, M. J. Kiel, and S. J. Morrison (2006)
Blood 107, 924-930
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Hematopoietic stem cell exhaustion impacted by p18INK4C and p21Cip1/Waf1 in opposite manners.
H. Yu, Y. Yuan, H. Shen, and T. Cheng (2006)
Blood 107, 1200-1206
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Purified hematopoietic stem cell engraftment of rare niches corrects severe lymphoid deficiencies without host conditioning.
D. Bhattacharya, D. J. Rossi, D. Bryder, and I. L. Weissman (2006)
J. Exp. Med. 203, 73-85
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Hematopoietic stem cells do not engraft with absolute efficiencies.
F. D. Camargo, S. M. Chambers, E. Drew, K. M. McNagny, and M. A. Goodell (2006)
Blood 107, 501-507
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Hematopoietic Stem Cells Are Not the Direct Target of Spontaneous Leukemic Transformation in p18INK4C-Null Reconstituted Mice.
Y. Yuan, H. Yu, M. J. Boyer, X. Song, S. Cao, H. Shen, and T. Cheng (2006)
Cancer Res. 66, 343-351
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