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Science 24 May 1996:
Vol. 272. no. 5265, pp. 1161 - 1163
DOI: 10.1126/science.272.5265.1161
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Reports

GS28, a 28-Kilodalton Golgi SNARE That Participates in ER-Golgi Transport

V. Nathan Subramaniam, Frank Peter, Robin Philp, Siew Heng Wong, Wanjin Hong *

Little is known about the integral membrane proteins that participate in the early secretory pathway of mammalian cells. The complementary DNA encoding a 28-kilodalton protein (p28) of the cis-Golgi was cloned and sequenced. The protein was predicted to contain a central coiled-coil domain with a carboxyl-terminal membrane anchor. An in vitro assay for endoplasmic reticulum-Golgi transport was used to show that p28 participates in the docking and fusion stage of this transport event. Biochemical studies established that p28 is a core component of the Golgi SNAP receptor (SNARE) complex.

Membrane Biology Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore.
* To whom correspondence should be addressed.


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Protein transport from the endoplasmic reticulum to the Golgi apparatus.
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F Peter, S. Wong, V. Subramaniam, B. Tang, and W Hong (1998)
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Recycling of the yeast v-SNARE Sec22p involves COPI-proteins and the ER transmembrane proteins Ufe1p and Sec20p.
W Ballensiefen, D Ossipov, and H. Schmitt (1998)
J. Cell Sci. 111, 1507-1520
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Syntaxin 7, a Novel Syntaxin Member Associated with the Early Endosomal Compartment.
S. H. Wong, Y. Xu, T. Zhang, and W. Hong (1998)
J. Biol. Chem. 273, 375-380
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The Mammalian Protein (rbet1) Homologous to Yeast Bet1p Is Primarily Associated with the Pre-Golgi Intermediate Compartment and Is Involved in Vesicular Transport from the Endoplasmic Reticulum to the Golgi Apparatus.
T. Zhang, S. H. Wong, B. L. Tang, Y. Xu, F. Peter, V. N. Subramaniam, and W. Hong (1997)
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Characterization of a Novel Yeast SNARE Protein Implicated in Golgi Retrograde Traffic.
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   Abstract »    Full Text »
N-Ethylmaleimide-sensitive Factor (NSF) and alpha -Soluble NSF Attachment Proteins (SNAP) Mediate Dissociation of GS28-Syntaxin 5 Golgi SNAP Receptors (SNARE) Complex.
V. N. Subramaniam, E. Loh, and W. Hong (1997)
J. Biol. Chem. 272, 25441-25444
   Abstract »    Full Text »    PDF »
GS15, a 15-Kilodalton Golgi Soluble N-Ethylmaleimide-sensitive Factor Attachment Protein Receptor (SNARE) Homologous to rbet1.
Y. Xu, S. H. Wong, T. Zhang, V. N. Subramaniam, and W. Hong (1997)
J. Biol. Chem. 272, 20162-20166
   Abstract »    Full Text »    PDF »
ERS-24, a Mammalian v-SNARE Implicated in Vesicle Traffic between the ER and the Golgi.
I. Paek, L. Orci, M. Ravazzola, H. Erdjument-Bromage, M. Amherdt, P. Tempst, T. H. Sollner, and J. E. Rothman (1997)
J. Cell Biol. 137, 1017-1028
   Abstract »    Full Text »    PDF »
The p115-interactive Proteins GM130 and Giantin Participate in Endoplasmic Reticulum-Golgi Traffic.
C. Alvarez, R. Garcia-Mata, H.-P. Hauri, and E. Sztul (2001)
J. Biol. Chem. 276, 2693-2700
   Abstract »    Full Text »    PDF »
Sequential Involvement of p115, SNAREs, and Rab Proteins in Intra-Golgi Protein Transport.
M. J. S. Gmachl and C. Wimmer (2001)
J. Biol. Chem. 276, 18178-18184
   Abstract »    Full Text »    PDF »
Ykt6 Forms a SNARE Complex with Syntaxin 5, GS28, and Bet1 and Participates in a Late Stage in Endoplasmic Reticulum-Golgi Transport.
T. Zhang and W. Hong (2001)
J. Biol. Chem. 276, 27480-27487
   Abstract »    Full Text »    PDF »
Anterograde flow of cargo across the Golgi stack potentially mediated via bidirectional "percolating" COPI vesicles.
L. Orci, M. Ravazzola, A. Volchuk, T. Engel, M. Gmachl, M. Amherdt, A. Perrelet, T. H. Sollner, and J. E. Rothman (2000)
PNAS 97, 10400-10405
   Abstract »    Full Text »    PDF »
Sorting of Golgi resident proteins into different subpopulations of COPI vesicles: a role for ArfGAP1.
J. Lanoix, J. Ouwendijk, A. Stark, E. Szafer, D. Cassel, K. Dejgaard, M. Weiss, and T. Nilsson (2001)
J. Cell Biol. 155, 1199-1212
   Abstract »    Full Text »    PDF »


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