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Science 10 May 1996:
Vol. 272. no. 5263, pp. 830 - 836
DOI: 10.1126/science.272.5263.830
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Research Articles

Crystal Structure of the Yeast TFIIA/TBP/DNA Complex

James H. Geiger, Steve Hahn, Sally Lee, Paul B. Sigler *

The crystal structure of the yeast TFIIA/TBP/TATA promoter complex was solved to 3 angstrom resolution by double-edge multiple wavelength anomalous diffraction from two different species of anomalous scattering elements in the same crystal. The large and small subunits of TFIIA associate intimately to form both domains of a two-domain folding pattern. TFIIA binds as a heterodimer to the side of the TBP/TATA complex opposite to the side that binds TFIIB and does not alter the TBP/DNA interaction. The six-stranded beta -sandwich domain interacts with the amino-terminal end of TBP through a stereospecific parallel beta -strand interface and with the backbone of the TATA box and the 5'-flanking B-DNA segment. The four-helix-bundle domain projects away from the TBP/TATA complex, thereby presenting a substantial surface for further protein-protein interactions.

J. H. Geiger and P. B. Sigler are in the Department of Molecular Biophysics and Biochemistry and the Howard Hughes Medical Institute, Yale University, New Haven, CT 06510, USA. S. Hahn and S. Lee are at the Fred Hutchinson Cancer Research Center, A1-162, Seattle, WA 98104, USA.
* To whom correspondence should be addressed.


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Transcription Factor IIA Derepresses TATA-binding Protein (TBP)-associated Factor Inhibition of TBP-DNA Binding.
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J. Biol. Chem. 273, 14293-14300
   Abstract »    Full Text »    PDF »
Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery.
M. Hampsey (1998)
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Regulation of gene expression by TBP-associated proteins.
T. I. Lee and R. A. Young (1998)
Genes & Dev. 12, 1398-1408
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Mol. Cell. Biol. 18, 2559-2570
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T. Colbert, S. Lee, G. Schimmack, and S. Hahn (1998)
Mol. Cell. Biol. 18, 1682-1691
   Abstract »    Full Text »
Polymerase (Pol) III TATA Box-Binding Protein (TBP)-Associated Factor Brf Binds to a Surface on TBP Also Required for Activated Pol II Transcription.
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Mol. Cell. Biol. 18, 1692-1700
   Abstract »    Full Text »
The Yeast TAF145 Inhibitory Domain and TFIIA Competitively Bind to TATA-Binding Protein.
T. Kokubo, M. J. Swanson, J.-i. Nishikawa, A. G. Hinnebusch, and Y. Nakatani (1998)
Mol. Cell. Biol. 18, 1003-1012
   Abstract »    Full Text »
RNA Polymerase-DNA Interaction: Structures of Intermediate, Open, and Elongation Complexes.
R.H. EBRIGHT (1998)
Cold Spring Harb Symp Quant Biol 63, 11-20
   Abstract »    PDF »
Polarity of Transcription on Pol II and Archaeal Promoters: Where Is the "One-way Sign" and How Is It Read?.
F.T.F. TSAI, O. LITTLEFIELD, P.F. KOSA, J.M. COX, A. SCHEPARTZ, and P.B. SIGLER (1998)
Cold Spring Harb Symp Quant Biol 63, 53-62
   Abstract »    PDF »
The RNA Polymerase II General Transcription Factors: Past, Present, and Future.
D. REINBERG, G. ORPHANIDES, R. EBRIGHT, S. AKOULITCHEV, J. CARCAMO, H. CHO, P. CORTES, R. DRAPKIN, O. FLORES, I. HA, et al. (1998)
Cold Spring Harb Symp Quant Biol 63, 83-105
   Abstract »    PDF »
Mechanisms of Viral Activators.
A.J. BERK, T.G. BOYER, A.N. KAPANIDIS, R.H. EBRIGHT, N.N. KOBAYASHI, P.J. HORN, S.M. SULLIVAN, R. KOOP, M.A. SURBY, and S.J. TRIEZENBERG (1998)
Cold Spring Harb Symp Quant Biol 63, 243-252
   Abstract »    PDF »
Activation and Repression Mechanisms in Yeast.
K. STRUHL, D. KADOSH, M. KEAVENEY, L. KURAS, and Z. MOQTADERI (1998)
Cold Spring Harb Symp Quant Biol 63, 413-422
   Abstract »    PDF »
Bidirectional binding of the TATA box binding protein to the TATA box.
J. M. Cox, M. M. Hayward, J. F. Sanchez, L. D. Gegnas, S. van der Zee, J. H. Dennis, P. B. Sigler, and A. Schepartz (1997)
PNAS 94, 13475-13480
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Trajectory of DNA in the RNA polymerase II transcription preinitiation complex.
T.-K. Kim, T. Lagrange, Y.-H. Wang, J. D. Griffith, D. Reinberg, and R. H. Ebright (1997)
PNAS 94, 12268-12273
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Considerations of transcriptional control mechanisms: Do TFIID-core promoter complexes recapitulate nucleosome-like functions?.
A. Hoffmann, T. Oelgeschlager, and R. G. Roeder (1997)
PNAS 94, 8928-8935
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Synergistic and promoter-selective activation of transcription by recruitment of transcription factors TFIID and TFIIB.
E. Gonzalez-Couto, N. Klages, and M. Strubin (1997)
PNAS 94, 8036-8041
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RAP74 induces promoter contacts by RNA polymerase II upstream and downstream of a DNA bend centered on the TATA box.
D. Forget, F. Robert, G. Grondin, Z. F. Burton, J. Greenblatt, and B. Coulombe (1997)
PNAS 94, 7150-7155
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The 2.1-A crystal structure of an archaeal preinitiation complex: TATA-box-binding protein/transcription factor (II)B core/TATA-box.
P. F. Kosa, G. Ghosh, B. S. DeDecker, and P. B. Sigler (1997)
PNAS 94, 6042-6047
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Local DNA stretching mimics the distortion caused by the TATA box-binding protein.
A. Lebrun, Z. Shakked, and R. Lavery (1997)
PNAS 94, 2993-2998
   Abstract »    Full Text »    PDF »
Protease Footprinting Analysis of Ternary Complex Formation by Human TFIIA.
R. Hori and M. Carey (1997)
J. Biol. Chem. 272, 1180-1187
   Abstract »    Full Text »    PDF »
RNA polymerase II transcription initiation: A structural view.
D. B. Nikolov and S. K. Burley (1997)
PNAS 94, 15-22
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A critical arginine residue mediates cooperativity in the contact interface between transcription factors NFAT and AP-1.
B. R. Peterson, L. J. Sun, and G. L. Verdine (1996)
PNAS 93, 13671-13676
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The general transcription factors of RNA polymerase II..
G Orphanides, T Lagrange, and D Reinberg (1996)
Genes & Dev. 10, 2657-2683
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Yeast HMG proteins NHP6A/B potentiate promoter-specific transcriptional activation in vivo and assembly of preinitiation complexes in vitro..
T T Paull, M Carey, and R C Johnson (1996)
Genes & Dev. 10, 2769-2781
   Abstract »    PDF »
Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo..
G O Bryant, L S Martel, S K Burley, and A J Berk (1996)
Genes & Dev. 10, 2491-2504
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Influence of HMG-1 and Adenovirus Oncoprotein E1A on Early Stages of Transcriptional Preinitiation Complex Assembly.
W. Lu, R. Peterson, A. Dasgupta, and W. M. Scovell (2000)
J. Biol. Chem. 275, 35006-35012
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TAFII 250 Phosphorylates Human Transcription Factor IIA on Serine Residues Important for TBP Binding and Transcription Activity.
S. Solow, M. Salunek, R. Ryan, and P. M. Lieberman (2001)
J. Biol. Chem. 276, 15886-15892
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High Affinity Interaction of Yeast Transcriptional Regulator, Mot1, with TATA Box-binding Protein (TBP).
J. I. Adamkewicz, K. E. Hansen, W. A. Prud'homme, J. L. Davis, and J. Thorner (2001)
J. Biol. Chem. 276, 11883-11894
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The Stability of the TFIIA-TBP-DNA Complex Is Dependent on the Sequence of the TATAAA Element.
J. J. Stewart and L. A. Stargell (2001)
J. Biol. Chem. 276, 30078-30084
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Fluorescence-based Analyses of the Effects of Full-length Recombinant TAF130p on the Interaction of TATA Box-binding Protein with TATA Box DNA.
U. Banik, J. M. Beechem, E. Klebanow, S. Schroeder, and P. A. Weil (2001)
J. Biol. Chem. 276, 49100-49109
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