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Science 26 April 1996:
Vol. 272. no. 5261, pp. 560 - 562
DOI: 10.1126/science.272.5261.560
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Reports

Linkage of Replication to Start by the Cdk Inhibitor Sic1

B. L. Schneider, * Q.-H. Yang, * A. B. Futcher dagger

In Saccharomyces cerevisiae, three G1 cyclins (Clns) are important for Start, the event committing cells to division. Sic1, an inhibitor of Clb-Cdc28 kinases, became phosphorylated at Start, and this phosphorylation depended on the activity of Clns. Sic1 was subsequently lost, which depended on the activity of Clns and the ubiquitin-conjugating enzyme Cdc34. Inactivation of Sic1 was the only nonredundant essential function of Clns, because a sic1 deletion rescued the inviability of the cln1 cln2 cln3 triple mutant. In sic1 mutants, DNA replication became uncoupled from budding. Thus, Sic1 may be a substrate of Cln-Cdc28 complexes, and phosphorylation and proteolysis of Sic1 may regulate commitment to replication at Start.

B. L. Schneider and A. B. Futcher, Post Office Box 100, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
Q.-H. Yang, Post Office Box 100, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA, and Graduate Program in Genetics, State University of New York, Stony Brook, NY 11794, USA.
* These authors contributed equally to this work.
dagger To whom correspondence should be addressed. E-mail: futcher{at}cshl.org

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