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Science 26 April 1996:
Vol. 272. no. 5261, pp. 533 - 535
DOI: 10.1126/science.272.5261.533
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Reports

Role of Type I Myosins in Receptor-Mediated Endocytosis in Yeast

M. Isabel Geli and Howard Riezman *

Type I myosins are thought to drive actin-dependent membrane motility, but the direct demonstration in vivo of their involvement in specific cellular processes has been difficult. Deletion of the genes MYO3 and MYO5, which encode the yeast type I myosins, almost abolished growth. A double-deleted mutant complemented with a MYO5 temperature-sensitive allele (myo5-1) showed a strong defect in the internalization step of receptor-mediated endocytosis, whereas the secretory pathway remained apparently unaffected. Thus, myosin I activity is required for a budding event in endocytosis but not for several other aspects of membrane traffic.

Department of Biochemistry, Biozentrum, University of Basel, Klingelbergstrasse 70, Basel, CH-4056, Switzerland.
* To whom correspondence should be addressed.

[Data]


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T Self, M Mahony, J Fleming, J Walsh, S. Brown, and K. Steel (1998)
Development 125, 557-566
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O. Mundigl, G.-C. Ochoa, C. David, V. I. Slepnev, A. Kabanov, and P. De Camilli (1998)
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Morphology of the Yeast Endocytic Pathway.
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Myosin VIIA Is Required for Aminoglycoside Accumulation in Cochlear Hair Cells.
G. P. Richardson, A. Forge, C. J. Kros, J. Fleming, S. D. M. Brown, and K. P. Steel (1997)
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The Phosphorylation Site for Ste20p-like Protein Kinases Is Essential for the Function of Myosin-I in Yeast.
C. Wu, V. Lytvyn, D. Y. Thomas, and E. Leberer (1997)
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End4p/Sla2p Interacts with Actin-associated Proteins for Endocytosis in Saccharomyces cerevisiae.
A. Wesp, L. Hicke, J. Palecek, R. Lombardi, T. Aust, A.L. Munn, and H. Riezman (1997)
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The Actin Cytoskeleton Is Required for Receptor-mediated Endocytosis in Mammalian Cells.
C. Lamaze, L. M. Fujimoto, H. L. Yin, and S. L. Schmid (1997)
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Myosin II Is Involved in the Production of Constitutive Transport Vesicles from the TGN.
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p21-activated kinase has substrate specificity similar to Acanthamoeba myosin I heavy chain kinase and activates Acanthamoeba myosin I.
H. Brzeska, U. G. Knaus, Z.-Y. Wang, G. M. Bokoch, and E. D. Korn (1997)
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A Subtil and A Dautry-Varsat (1997)
J. Cell Sci. 110, 2441-2447
   Abstract »    PDF »
Activation of Myosin-I by Members of the Ste20p Protein Kinase Family.
C. Wu, S.-F. Lee, E. Furmaniak-Kazmierczak, G. P. Cote, D. Y. Thomas, and E. Leberer (1996)
J. Biol. Chem. 271, 31787-31790
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Cloning and Characterization of a Dictyostelium Myosin I Heavy Chain Kinase Activated by Cdc42 and Rac.
S.-F. Lee, T. T. Egelhoff, A. Mahasneh, and G. P. Cote (1996)
J. Biol. Chem. 271, 27044-27048
   Abstract »    Full Text »    PDF »
The Catalytic Domain of Acanthamoeba Myosin I Heavy Chain Kinase. II. EXPRESSION OF ACTIVE CATALYTIC DOMAIN AND SEQUENCE HOMOLOGY TO p21-ACTIVATED KINASE (PAK).
H. Brzeska, J. Szczepanowska, J. Hoey, and E. D. Korn (1996)
J. Biol. Chem. 271, 27056-27062
   Abstract »    Full Text »    PDF »
Recruitment of a Specific Amoeboid Myosin I Isoform to the Plasma Membrane in Chemotactic Dictyostelium Cells.
S. Senda, S.-F. Lee, G. P. Cote, and M. A. Titus (2001)
J. Biol. Chem. 276, 2898-2904
   Abstract »    Full Text »    PDF »


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