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Science 12 April 1996:
Vol. 272. no. 5259, pp. 279 - 281
DOI: 10.1126/science.272.5259.279
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Reports

Identification of Yeast Rho1p GTPase as a Regulatory Subunit of 1,3-beta -Glucan Synthase

Hiroshi Qadota, * Christophe P. Python, * Shunsuke B. Inoue, * Mikio Arisawa, Yasuhiro Anraku, Yi Zheng, Takahide Watanabe, David E. Levin, Yoshikazu Ohya dagger

1,3-beta -D-Glucan synthase [also known as beta (1rightarrow 3)glucan synthase] is a multi-enzyme complex that catalyzes the synthesis of 1,3-beta -linked glucan, a major structural component of the yeast cell wall. Temperature-sensitive mutants in the essential Rho-type guanosine triphosphatase (GTPase), Rho1p, displayed thermolabile glucan synthase activity, which was restored by the addition of recombinant Rho1p. Glucan synthase from mutants expressing constitutively active Rho1p did not require exogenous guanosine triphosphate for activity. Rho1p copurified with beta (1rightarrow 3)glucan synthase and associated with the Fks1p subunit of this complex in vivo. Both proteins were localized predominantly at sites of cell wall remodeling. Therefore, it appears that Rho1p is a regulatory subunit of beta (1rightarrow 3)glucan synthase.

H. Qadota, Y. Anraku, Y. Ohya, Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan.
C. P. Python and D. E. Levin, Department of Biochemistry, Johns Hopkins University School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA.
S. B. Inoue, M. Arisawa, T. Watanabe, Department of Mycology, Nippon Roche Research Center, 200. Kajiwara, Kamakura, Kanagawa Prefecture 247, Japan.
Y. Zheng, Department of Biochemistry, University of Tennessee, Memphis, TN 38163, USA.
* These authors contributed equally to this work.
dagger To whom correspondence should be addressed.


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A Septin-based Hierarchy of Proteins Required for Localized Deposition of Chitin in the Saccharomyces cerevisiae Cell Wall.
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Bni1p, a Yeast Formin Linking Cdc42p and the Actin Cytoskeleton During Polarized Morphogenesis.
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Yeast 1,3-beta -Glucan Synthase Activity Is Inhibited by Phytosphingosine Localized to the Endoplasmic Reticulum.
M. Abe, I. Nishida, M. Minemura, H. Qadota, Y. Seyama, T. Watanabe, and Y. Ohya (2001)
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Molecular Organization of the Alkali-insoluble Fraction of Aspergillus fumigatus Cell Wall.
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