Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 12 April 1996:
Vol. 272. no. 5259, pp. 277 - 279
DOI: 10.1126/science.272.5259.277
Prev | Table of Contents | Next

Reports

Rho1p, a Yeast Protein at the Interface Between Cell Polarization and Morphogenesis

Jana Drgonová, Tomás Drgon, Kazuma Tanaka, Roman Kollár, Guang-Chao Chen, Richard A. Ford, Clarence S. M. Chan, Yoshimi Takai, Enrico Cabib *

The enzyme that catalyzes the synthesis of the major structural component of the yeast cell wall, beta (1rightarrow 3)-D-glucan synthase (also known as 1,3-beta -glucan synthase), requires a guanosine triphosphate (GTP) binding protein for activity. The GTP binding protein was identified as Rho1p. The rho1 mutants were defective in GTP stimulation of glucan synthase, and the defect was corrected by addition of purified or recombinant Rho1p. A protein missing in purified preparations from a rho1 strain was identified as Rho1p. Rho1p also regulates protein kinase C, which controls a mitogen-activated protein kinase cascade. Experiments with a dominant positive PKC1 gene showed that the two effects of Rho1p are independent of each other. The colocalization of Rho1p with actin patches at the site of bud emergence and the role of Rho1p in cell wall synthesis emphasize the importance of Rho1p in polarized growth and morphogenesis.

J. Drgonová, T. Drgon, R. Kollár, R. A. Ford, E. Cabib, Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, Building 10, Room 9N-115, Bethesda, MD 20892, USA.
K. Tanaka and Y. Takai, Department of Molecular Biology and Biochemistry, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565, Japan.
G.-C. Chen and C. S. M. Chan, Department of Microbiology, University of Texas, Austin, TX 78712, USA.
* To whom correspondence should be addressed.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Our Paths Might Cross: the Role of the Fungal Cell Wall Integrity Pathway in Stress Response and Cross Talk with Other Stress Response Pathways.
B. B. Fuchs and E. Mylonakis (2009)
Eukaryot. Cell 8, 1616-1625
   Abstract »    Full Text »    PDF »
Comparative Proteomic Analysis of Tolerance and Adaptation of Ethanologenic Saccharomyces cerevisiae to Furfural, a Lignocellulosic Inhibitory Compound.
F.-M. Lin, B. Qiao, and Y.-J. Yuan (2009)
Appl. Envir. Microbiol. 75, 3765-3776
   Abstract »    Full Text »    PDF »
The function of two closely related Rho proteins is determined by an atypical switch I region.
M. Kohli, S. Buck, and H.-P. Schmitz (2008)
J. Cell Sci. 121, 1065-1075
   Abstract »    Full Text »    PDF »
G1/S Cyclin-dependent Kinase Regulates Small GTPase Rho1p through Phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae.
K. Kono, S. Nogami, M. Abe, M. Nishizawa, S. Morishita, D. Pellman, and Y. Ohya (2008)
Mol. Biol. Cell 19, 1763-1771
   Abstract »    Full Text »    PDF »
An Anti-{beta}-Glucan Monoclonal Antibody Inhibits Growth and Capsule Formation of Cryptococcus neoformans In Vitro and Exerts Therapeutic, Anticryptococcal Activity In Vivo.
A. Rachini, D. Pietrella, P. Lupo, A. Torosantucci, P. Chiani, C. Bromuro, C. Proietti, F. Bistoni, A. Cassone, and A. Vecchiarelli (2007)
Infect. Immun. 75, 5085-5094
   Abstract »    Full Text »    PDF »
Susceptibility of Cryptococcus neoformans to Photodynamic Inactivation Is Associated with Cell Wall Integrity.
B. B. Fuchs, G. P. Tegos, M. R. Hamblin, and E. Mylonakis (2007)
Antimicrob. Agents Chemother. 51, 2929-2936
   Abstract »    Full Text »    PDF »
Up-regulation of the Cell Integrity Pathway in Saccharomyces cerevisiae Suppresses Temperature Sensitivity of the pgs1{Delta} Mutant.
Q. Zhong, G. Li, J. Gvozdenovic-Jeremic, and M. L. Greenberg (2007)
J. Biol. Chem. 282, 15946-15953
   Abstract »    Full Text »    PDF »
Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond.
H.-O. Park and E. Bi (2007)
Microbiol. Mol. Biol. Rev. 71, 48-96
   Abstract »    Full Text »    PDF »
Homologous Subunits of 1,3-Beta-Glucan Synthase Are Important for Spore Wall Assembly in Saccharomyces cerevisiae.
S. Ishihara, A. Hirata, S. Nogami, A. Beauvais, J.-P. Latge, and Y. Ohya (2007)
Eukaryot. Cell 6, 143-156
   Abstract »    Full Text »    PDF »
NPFXD-mediated Endocytosis Is Required for Polarity and Function of a Yeast Cell Wall Stress Sensor.
H. L. Piao, I. M.P. Machado, and G. S. Payne (2007)
Mol. Biol. Cell 18, 57-65
   Abstract »    Full Text »    PDF »
The Cdc34/SCF Ubiquitination Complex Mediates Saccharomyces cerevisiae Cell Wall Integrity.
X. Varelas, D. Stuart, M. J. Ellison, and C. Ptak (2006)
Genetics 174, 1825-1839
   Abstract »    Full Text »    PDF »
KlRHO1 and KlPKC1 are essential for cell integrity signalling in Kluyveromyces lactis..
R. Rodicio, S. Koch, H.-P. Schmitz, and J. J. Heinisch (2006)
Microbiology 152, 2635-2649
   Abstract »    Full Text »    PDF »
Role of Cell Cycle-regulated Expression in the Localized Incorporation of Cell Wall Proteins in Yeast.
G. J. Smits, L. R. Schenkman, S. Brul, J. R. Pringle, and F. M. Klis (2006)
Mol. Biol. Cell 17, 3267-3280
   Abstract »    Full Text »    PDF »
Cell Wall Assembly in Saccharomyces cerevisiae.
G. Lesage and H. Bussey (2006)
Microbiol. Mol. Biol. Rev. 70, 317-343
   Abstract »    Full Text »    PDF »
Cryptococcus neoformans Resistance to Echinocandins: (1,3){beta}-Glucan Synthase Activity Is Sensitive to Echinocandins.
M. A. Maligie and C. P. Selitrennikoff (2005)
Antimicrob. Agents Chemother. 49, 2851-2856
   Abstract »    Full Text »    PDF »
Cell Wall Integrity Signaling in Saccharomyces cerevisiae.
D. E. Levin (2005)
Microbiol. Mol. Biol. Rev. 69, 262-291
   Abstract »    Full Text »    PDF »
Pkc1 and the Upstream Elements of the Cell Integrity Pathway in Saccharomyces cerevisiae, Rom2 and Mtl1, Are Required for Cellular Responses to Oxidative Stress.
F. Vilella, E. Herrero, J. Torres, and M. A. de la Torre-Ruiz (2005)
J. Biol. Chem. 280, 9149-9159
   Abstract »    Full Text »    PDF »
Loss of Function of KRE5 Suppresses Temperature Sensitivity of Mutants Lacking Mitochondrial Anionic Lipids.
Q. Zhong, J. Gvozdenovic-Jeremic, P. Webster, J. Zhou, and M. L. Greenberg (2005)
Mol. Biol. Cell 16, 665-675
   Abstract »    Full Text »    PDF »
The novel fission yeast (1,3){beta}-D-glucan synthase catalytic subunit Bgs4p is essential during both cytokinesis and polarized growth.
J. C. G. Cortes, E. Carnero, J. Ishiguro, Y. Sanchez, A. Duran, and J. C. Ribas (2005)
J. Cell Sci. 118, 157-174
   Abstract »    Full Text »    PDF »
Genomic Approach to Identification of Mutations Affecting Caspofungin Susceptibility in Saccharomyces cerevisiae.
S. Markovich, A. Yekutiel, I. Shalit, Y. Shadkchan, and N. Osherov (2004)
Antimicrob. Agents Chemother. 48, 3871-3876
   Abstract »    Full Text »    PDF »
Mutational analysis of the cytoplasmic domain of the Wsc1 cell wall stress sensor.
H. A. Vay, B. Philip, and D. E. Levin (2004)
Microbiology 150, 3281-3288
   Abstract »    Full Text »    PDF »
Lrg1p Is a Rho1 GTPase-Activating Protein Required for Efficient Cell Fusion in Yeast.
P. G. Fitch, A. E. Gammie, D. J. Lee, V. B. de Candal, and M. D. Rose (2004)
Genetics 168, 733-746
   Abstract »    Full Text »    PDF »
Negative Regulation of Phosphatidylinositol 4,5-Bisphosphate Levels by the INP51-associated Proteins TAX4 and IRS4.
H. Morales-Johansson, P. Jenoe, F. T. Cooke, and M. N. Hall (2004)
J. Biol. Chem. 279, 39604-39610
   Abstract »    Full Text »    PDF »
Pxl1p, a Paxillin-like Protein in Saccharomyces cerevisiae, May Coordinate Cdc42p and Rho1p Functions during Polarized Growth.
X.-D. Gao, J. P. Caviston, S. E. Tcheperegine, and E. Bi (2004)
Mol. Biol. Cell 15, 3977-3985
   Abstract »    Full Text »    PDF »
Discovery of Cercosporamide, a Known Antifungal Natural Product, as a Selective Pkc1 Kinase Inhibitor through High-Throughput Screening.
A. Sussman, K. Huss, L.-C. Chio, S. Heidler, M. Shaw, D. Ma, G. Zhu, R. M. Campbell, T.-S. Park, P. Kulanthaivel, et al. (2004)
Eukaryot. Cell 3, 932-943
   Abstract »    Full Text »    PDF »
Analysis of {beta}-1,3-Glucan Assembly in Saccharomyces cerevisiae Using a Synthetic Interaction Network and Altered Sensitivity to Caspofungin.
G. Lesage, A.-M. Sdicu, P. Menard, J. Shapiro, S. Hussein, and H. Bussey (2004)
Genetics 167, 35-49
   Abstract »    Full Text »    PDF »
The p21-activated Protein Kinase-related Kinase Cla4 Is a Coincidence Detector of Signaling by Cdc42 and Phosphatidylinositol 4-Phosphate.
A. C. Wild, J. W. Yu, M. A. Lemmon, and K. J. Blumer (2004)
J. Biol. Chem. 279, 17101-17110
   Abstract »    Full Text »    PDF »
The PXL1 Gene of Saccharomyces cerevisiae Encodes a Paxillin-like Protein Functioning in Polarized Cell Growth.
N. A. Mackin, T. J. Sousou, and S. E. Erdman (2004)
Mol. Biol. Cell 15, 1904-1917
   Abstract »    Full Text »    PDF »
Differential Gene Expression in Auristatin PHE-Treated Cryptococcus neoformans.
T. Woyke, M. E. Berens, D. B. Hoelzinger, G. R. Pettit, G. Winkelmann, and R. K. Pettit (2004)
Antimicrob. Agents Chemother. 48, 561-567
   Abstract »    Full Text »    PDF »
The Ras/Protein Kinase A Pathway Acts in Parallel with the Mob2/Cbk1 Pathway To Effect Cell Cycle Progression and Proper Bud Site Selection.
L. Schneper, A. Krauss, R. Miyamoto, S. Fang, and J. R. Broach (2004)
Eukaryot. Cell 3, 108-120
   Abstract »    Full Text »    PDF »
Receptor Internalization in Yeast Requires the Tor2-Rho1 Signaling Pathway.
A. K.A. deHart, J. D. Schnell, D. A. Allen, J.-Y. Tsai, and L. Hicke (2003)
Mol. Biol. Cell 14, 4676-4684
   Abstract »    Full Text »    PDF »
Lack of GTP-bound Rho1p in secretory vesicles of Saccharomyces cerevisiae.
M. Abe, H. Qadota, A. Hirata, and Y. Ohya (2003)
J. Cell Biol. 162, 85-97
   Abstract »    Full Text »    PDF »
G{beta}{gamma} Recruits Rho1 to the Site of Polarized Growth during Mating in Budding Yeast.
E. E. Bar, A. T. Ellicott, and D. E. Stone (2003)
J. Biol. Chem. 278, 21798-21804
   Abstract »    Full Text »    PDF »
Bgs3p, a Putative 1,3-{beta}-Glucan Synthase Subunit, Is Required for Cell Wall Assembly in Schizosaccharomyces pombe.
V. Martin, B. Garcia, E. Carnero, A. Duran, and Y. Sanchez (2003)
Eukaryot. Cell 2, 159-169
   Abstract »    Full Text »    PDF »
Cdc50p, a Conserved Endosomal Membrane Protein, Controls Polarized Growth in Saccharomyces cerevisiae.
K. Misu, K. Fujimura-Kamada, T. Ueda, A. Nakano, H. Katoh, and K. Tanaka (2003)
Mol. Biol. Cell 14, 730-747
   Abstract »    Full Text »    PDF »
RAM2: an essential gene in the prenylation pathway of Candida albicans.
J. L. Song and T. C. White (2003)
Microbiology 149, 249-259
   Abstract »    Full Text »    PDF »
Endo/exocytosis in the pollen tube apex is differentially regulated by Ca2+ and GTPases.
L. Camacho and R. Malho (2003)
J. Exp. Bot. 54, 83-92
   Abstract »    Full Text »    PDF »
Localization of the (1,3){beta}-D-glucan synthase catalytic subunit homologue Bgs1p/Cps1p from fission yeast suggests that it is involved in septation, polarized growth, mating, spore wall formation and spore germination.
J. C. G. Cortes, J. Ishiguro, A. Duran, and J. C. Ribas (2002)
J. Cell Sci. 115, 4081-4096
   Abstract »    Full Text »    PDF »
Differential Sensitivity between Fks1p and Fks2p against a Novel beta -1,3-Glucan Synthase Inhibitor, Aerothricin1.
O. Kondoh, T. Takasuka, M. Arisawa, Y. Aoki, and T. Watanabe (2002)
J. Biol. Chem. 277, 41744-41749
   Abstract »    Full Text »    PDF »
Dissection of Upstream Regulatory Components of the Rho1p Effector, 1,3-{beta}-Glucan Synthase, in Saccharomyces cerevisiae.
M. Sekiya-Kawasaki, M. Abe, A. Saka, D. Watanabe, K. Kono, M. Minemura-Asakawa, S. Ishihara, T. Watanabe, and Y. Ohya (2002)
Genetics 162, 663-676
   Abstract »    Full Text »    PDF »
Osmotic Stress Signaling and Osmoadaptation in Yeasts.
S. Hohmann (2002)
Microbiol. Mol. Biol. Rev. 66, 300-372
   Abstract »    Full Text »    PDF »
Yeast Protein Kinases and the RHO1 Exchange Factor TUS1 Are Novel Components of the Cell Integrity Pathway in Yeast.
T. Schmelzle, S. B. Helliwell, and M. N. Hall (2002)
Mol. Cell. Biol. 22, 1329-1339
   Abstract »    Full Text »    PDF »
The Localization of the Integral Membrane Protein Cps1p to the Cell Division Site is Dependent on the Actomyosin Ring and the Septation-Inducing Network in Schizosaccharomyces pombe.
J. Liu, X. Tang, H. Wang, S. Oliferenko, and M. K. Balasubramanian (2002)
Mol. Biol. Cell 13, 989-1000
   Abstract »    Full Text »    PDF »
Yeast Rpi1 Is a Putative Transcriptional Regulator That Contributes to Preparation for Stationary Phase.
A. K. Sobering, U. S. Jung, K. S. Lee, and D. E. Levin (2002)
Eukaryot. Cell 1, 56-65
   Abstract »    Full Text »    PDF »
The small GTPase Rho3 and the diaphanous/formin For3 function in polarized cell growth in fission yeast.
K. Nakano, J. Imai, R. Arai, A. Toh-e, Y. Matsui, and I. Mabuchi (2002)
J. Cell Sci. 115, 4629-4639
   Abstract »    Full Text »    PDF »
Rho5p downregulates the yeast cell integrity pathway.
H.-P. Schmitz, S. Huppert, A. Lorberg, and J. J. Heinisch (2002)
J. Cell Sci. 115, 3139-3148
   Abstract »    Full Text »    PDF »
Overactivation of the Protein Kinase C-Signaling Pathway Suppresses the Defects of Cells Lacking the Rho3/Rho4-GAP Rgd1p in Saccharomyces cerevisiae.
G. de Bettignies, D. Thoraval, C. Morel, M. F. Peypouquet, and M. Crouzet (2001)
Genetics 159, 1435-1448
   Abstract »    Full Text »    PDF »
Complementing Yeast rho1 Mutation Groups with Distinct Functional Defects.
A. Saka, M. Abe, H. Okano, M. Minemura, H. Qadota, T. Utsugi, A. Mino, K. Tanaka, Y. Takai, and Y. Ohya (2001)
J. Biol. Chem. 276, 46165-46171
   Abstract »    Full Text »    PDF »
A protein interaction map for cell polarity development.
B. L. Drees, B. Sundin, E. Brazeau, J. P. Caviston, G.-C. Chen, W. Guo, K. G. Kozminski, M. W. Lau, J. J. Moskow, A. Tong, et al. (2001)
J. Cell Biol. 154, 549-576
   Abstract »    Full Text »    PDF »
Induction of a Mitosis Delay and Cell Lysis by High-Level Secretion of Mouse {alpha}-Amylase from Saccharomyces cerevisiae.
B.-D. Wang and T.-T. Kuo (2001)
Appl. Envir. Microbiol. 67, 3693-3701
   Abstract »    Full Text »    PDF »
Cytokinesis in Prokaryotes and Eukaryotes: Common Principles and Different Solutions.
N. Nanninga (2001)
Microbiol. Mol. Biol. Rev. 65, 319-333
   Abstract »    Full Text »    PDF »
Cell cycle control of septin ring dynamics in the budding yeast.
V. J. Cid, L. Adamikova, M. Sanchez, M. Molina, and C. Nombela (2001)
Microbiology 147, 1437-1450
   Abstract »    Full Text »    PDF »
Dynamic Localization and Function of Bni1p at the Sites of Directed Growth in Saccharomyces cerevisiae.
K. Ozaki-Kuroda, Y. Yamamoto, H. Nohara, M. Kinoshita, T. Fujiwara, K. Irie, and Y. Takai (2001)
Mol. Cell. Biol. 21, 827-839
   Abstract »    Full Text »
Wsc1 and Mid2 Are Cell Surface Sensors for Cell Wall Integrity Signaling That Act through Rom2, a Guanine Nucleotide Exchange Factor for Rho1.
B. Philip and D. E. Levin (2001)
Mol. Cell. Biol. 21, 271-280
   Abstract »    Full Text »
Small GTP-Binding Proteins.
Y. Takai, T. Sasaki, and T. Matozaki (2001)
Physiol Rev 81, 153-208
   Abstract »    Full Text »    PDF »
Genetic Evidence for a Morphogenetic Function of the Saccharomyces cerevisiae Pho85 Cyclin-Dependent Kinase.
M. E. Lenburg and E. K. O'Shea (2001)
Genetics 157, 39-51
   Abstract »    Full Text »
Suppression of the Profilin-Deficient Phenotype by the RHO2 Signaling Pathway in Saccharomyces cerevisiae.
N. Marcoux, S. Cloutier, E. Zakrzewska, P.-M. Charest, Y. Bourbonnais, and D. Pallotta (2000)
Genetics 156, 579-592
   Abstract »    Full Text »
Properties of Various RHO1 Mutant Alleles of Cryptococcus neoformans.
Y. C. Chang and L. A. Penoyer (2000)
J. Bacteriol. 182, 4987-4991
   Abstract »    Full Text »
Geranylgeranyltransferase I of Candida albicans: Null Mutants or Enzyme Inhibitors Produce Unexpected Phenotypes.
R. Kelly, D. Card, E. Register, P. Mazur, T. Kelly, K.-I. Tanaka, J. Onishi, J. M. Williamson, H. Fan, T. Satoh, et al. (2000)
J. Bacteriol. 182, 704-713
   Abstract »    Full Text »
Type 1 protein phosphatase is required for maintenance of cell wall integrity, morphogenesis and cell cycle progression in Saccharomyces cerevisiae.
P. Andrews and M. Stark (2000)
J. Cell Sci. 113, 507-520
   Abstract »    PDF »
Pan1p, End3p, and Sla1p, Three Yeast Proteins Required for Normal Cortical Actin Cytoskeleton Organization, Associate with Each Other and Play Essential Roles in Cell Wall Morphogenesis.
H.-Y. Tang, J. Xu, and M. Cai (2000)
Mol. Cell. Biol. 20, 12-25
   Abstract »    Full Text »
Prenylation of Rho1p Is Required for Activation of Yeast 1,3-beta -Glucan Synthase.
S. B. Inoue, H. Qadota, M. Arisawa, T. Watanabe, and Y. Ohya (1999)
J. Biol. Chem. 274, 38119-38124
   Abstract »    Full Text »    PDF »
The Rho GTPase Rho3 Has a Direct Role in Exocytosis That Is Distinct from Its Role in Actin Polarity.
J. E. Adamo, G. Rossi, and P. Brennwald (1999)
Mol. Biol. Cell 10, 4121-4133
   Abstract »    Full Text »
Drc1p/Cps1p, a 1,3-{beta}-Glucan Synthase Subunit, Is Essential for Division Septum Assembly in Schizosaccharomyces pombe.
J. Liu, H. Wang, D. McCollum, and M. K. Balasubramanian (1999)
Genetics 153, 1193-1203
   Abstract »    Full Text »
Cell Wall Stress Depolarizes Cell Growth via Hyperactivation of Rho1.
P.-A. Delley and M. N. Hall (1999)
J. Cell Biol. 147, 163-174
   Abstract »    Full Text »    PDF »
Characterization of the Wsc1 Protein, a Putative Receptor in the Stress Response of Saccharomyces cerevisiae.
A. L. Lodder, T. K. Lee, and R. Ballester (1999)
Genetics 152, 1487-1499
   Abstract »    Full Text »
A 110-Kilodalton Subunit of Translation Initiation Factor eIF3 and an Associated 135-kilodalton Protein Are Encoded by the Saccharomyces cerevisiae TIF32 and TIF31 Genes.
H.-P. Vornlocher, P. Hanachi, S. Ribeiro, and J. W. B. Hershey (1999)
J. Biol. Chem. 274, 16802-16812
   Abstract »    Full Text »    PDF »
Mid2 Is a Putative Sensor for Cell Integrity Signaling in Saccharomyces cerevisiae.
M. Rajavel, B. Philip, B. M. Buehrer, B. Errede, and D. E. Levin (1999)
Mol. Cell. Biol. 19, 3969-3976
   Abstract »    Full Text »    PDF »
Saccharomyces cerevisiae Mid2p Is a Potential Cell Wall Stress Sensor and Upstream Activator of the PKC1-MPK1 Cell Integrity Pathway.
T. Ketela, R. Green, and H. Bussey (1999)
J. Bacteriol. 181, 3330-3340
   Abstract »    Full Text »
Rho3 of Saccharomyces cerevisiae, Which Regulates the Actin Cytoskeleton and Exocytosis, Is a GTPase Which Interacts with Myo2 and Exo70.
N. G. G. Robinson, L. Guo, J. Imai, A. Toh-e, Y. Matsui, and F. Tamanoi (1999)
Mol. Cell. Biol. 19, 3580-3587
   Abstract »    Full Text »    PDF »
Sla1p Is a Functionally Modular Component of the Yeast Cortical Actin Cytoskeleton Required for Correct Localization of Both Rho1p-GTPase and Sla2p, a Protein with Talin Homology.
K. R. Ayscough, J. J. Eby, T. Lila, H. Dewar, K. G. Kozminski, and D. G. Drubin (1999)
Mol. Biol. Cell 10, 1061-1075
   Abstract »    Full Text »
Fission Yeast {alpha}-Glucan Synthase Mok1 Requires the Actin Cytoskeleton to Localize the Sites of Growth and Plays an Essential Role in Cell Morphogenesis Downstream of Protein Kinase C Function.
S. Katayama, D. Hirata, M. Arellano, P. Perez, and T. Toda (1999)
J. Cell Biol. 144, 1173-1186
   Abstract »    Full Text »    PDF »
Amphotericin B and Fluconazole Affect Cellular Charge, Macrophage Phagocytosis, and Cellular Morphology of Cryptococcus neoformans at Subinhibitory Concentrations.
J. D. Nosanchuk, W. Cleare, S. P. Franzot, and A. Casadevall (1999)
Antimicrob. Agents Chemother. 43, 233-239
   Abstract »    Full Text »
A Glucan Synthase FKS1 Homolog in Cryptococcus neoformans Is Single Copy and Encodes an Essential Function.
J. R. Thompson, C. M. Douglas, W. Li, C. K. Jue, B. Pramanik, X. Yuan, T. H. Rude, D. L. Toffaletti, J. R. Perfect, and M. Kurtz (1999)
J. Bacteriol. 181, 444-453
   Abstract »    Full Text »
Schizosaccharomyces pombe protein kinase C homologues, pck1p and pck2p, are targets of rho1p and rho2p and differentially regulate cell integrity.
M Arellano, M. Valdivieso, T. Calonge, P. Coll, A Duran, and P Perez (1999)
J. Cell Sci. 112, 3569-3578
   Abstract »    PDF »
MAP Kinase Pathways in the Yeast Saccharomyces cerevisiae.
M. C. Gustin, J. Albertyn, M. Alexander, and K. Davenport (1998)
Microbiol. Mol. Biol. Rev. 62, 1264-1300
   Abstract »    Full Text »    PDF »
The Role of Glucosidase I (Cwh41p) in the Biosynthesis of Cell Wall beta -1,6-Glucan Is Indirect.
C. Abeijon and L. Y. Chen (1998)
Mol. Biol. Cell 9, 2729-2738
   Abstract »    Full Text »
Relocation of a Ca2+-Dependent Protein Kinase Activity during Pollen Tube Reorientation.
A. Moutinho, A. J. Trewavas, and R. Malhó (1998)
PLANT CELL 10, 1499-1510
   Abstract »    Full Text »
Signalling in the Yeasts: An Informational Cascade with Links to the Filamentous Fungi.
F. Banuett (1998)
Microbiol. Mol. Biol. Rev. 62, 249-274
   Abstract »    Full Text »    PDF »
Photoaffinity Analog of the Semisynthetic Echinocandin LY303366: Identification of Echinocandin Targets in Candida albicans.
J. A. Radding, S. A. Heidler, and W. W. Turner (1998)
Antimicrob. Agents Chemother. 42, 1187-1194
   Abstract »    Full Text »
Rho1p-Bni1p-Spa2p Interactions: Implication in Localization of Bni1p at the Bud Site and Regulation of the Actin Cytoskeleton in Saccharomyces cerevisiae.
T. Fujiwara, K. Tanaka, A. Mino, M. Kikyo, K. Takahashi, K. Shimizu, and Y. Takai (1998)
Mol. Biol. Cell 9, 1221-1233
   Abstract »    Full Text »
Cdc1 Is Required for Growth and Mn2+ Regulation in Saccharomyces cerevisiae.
M. Paidhungat and S. Garrett (1998)
Genetics 148, 1777-1786
   Abstract »    Full Text »    PDF »
Loss of the Plasma Membrane-Bound Protein Gas1p in Saccharomyces cerevisiae Results in the Release of beta 1,3-Glucan into the Medium and Induces a Compensation Mechanism To Ensure Cell Wall Integrity.
A. F. J. Ram, J. C. Kapteyn, R. C. Montijn, L. H. P. Caro, J. E. Douwes, W. Baginsky, P. Mazur, H. van den Ende, and F. M. Klis (1998)
J. Bacteriol. 180, 1418-1424
   Abstract »    Full Text »
Temperature-Induced Expression of Yeast FKS2 Is under the Dual Control of Protein Kinase C and Calcineurin.
C. Zhao, U. S. Jung, P. Garrett-Engele, T. Roe, M. S. Cyert, and D. E. Levin (1998)
Mol. Cell. Biol. 18, 1013-1022
   Abstract »    Full Text »
Rho GTPases and the Actin Cytoskeleton.
A. Hall (1998)
Science 279, 509-514
   Abstract »    Full Text »
Genes that cause aberrant cell morphology by overexpression in fission yeast: a role of a small GTP-binding protein Rho2 in cell morphogenesis.
D Hirata, K Nakano, M Fukui, H Takenaka, T Miyakawa, and I Mabuchi (1998)
J. Cell Sci. 111, 149-159
   Abstract »    PDF »
A family of genes required for maintenance of cell wall integrity and for the stress response in cerevisiae.
J. Verna, A. Lodder, K. Lee, A. Vagts, and R. Ballester (1997)
PNAS 94, 13804-13809
   Abstract »    Full Text »    PDF »
The Rho-GEF Rom2p Localizes to Sites of Polarized Cell Growth and Participates in Cytoskeletal Functions in Saccharomyces cerevisiae.
B. D. Manning, R. Padmanabha, and M. Snyder (1997)
Mol. Biol. Cell 8, 1829-1844
   Abstract »    Full Text »
Rho GTPases and signaling networks.
L. Van Aelst and C. D'Souza-Schorey (1997)
Genes & Dev. 11, 2295-2322
   Full Text »    PDF »
Architecture of the Yeast Cell Wall. beta (1right-arrow 6)-GLUCAN INTERCONNECTS MANNOPROTEIN, beta (1right-arrow 3)-GLUCAN, AND CHITIN.
R. Kollar, B. B. Reinhold, E. Petrakova, H. J. C. Yeh, G. Ashwell, J. Drgonova, J. C. Kapteyn, F. M. Klis, and E. Cabib (1997)
J. Biol. Chem. 272, 17762-17775
   Abstract »    Full Text »    PDF »
Bni1p, a Yeast Formin Linking Cdc42p and the Actin Cytoskeleton During Polarized Morphogenesis.
M. Evangelista, K. Blundell, M. S. Longtine, C. J. Chow, N. Adames, J. R. Pringle, M. Peter, and C. Boone (1997)
Science 276, 118-122
   Abstract »    Full Text »
Localisation of the Schizosaccharomyces pombe rho1p GTPase and its involvement in the organisation of the actin cytoskeleton.
M Arellano, A Duran, and P Perez (1997)
J. Cell Sci. 110, 2547-2555
   Abstract »    PDF »
Cortical Asymmetries Direct the Establishment of Cell Polarity and the Plane of Cell Division in the Fucus Embryo.
R.S. Quatrano (1997)
Cold Spring Harb Symp Quant Biol 62, 65-70
   Abstract »    PDF »
In Vitro Activity of 1,3-beta -D-Glucan Synthase Requires the GTP-binding Protein Rho1.
P. Mazur and W. Baginsky (1996)
J. Biol. Chem. 271, 14604-14609
   Abstract »    Full Text »    PDF »
Yeast 1,3-beta -Glucan Synthase Activity Is Inhibited by Phytosphingosine Localized to the Endoplasmic Reticulum.
M. Abe, I. Nishida, M. Minemura, H. Qadota, Y. Seyama, T. Watanabe, and Y. Ohya (2001)
J. Biol. Chem. 276, 26923-26930
   Abstract »    Full Text »    PDF »
Molecular Organization of the Alkali-insoluble Fraction of Aspergillus fumigatus Cell Wall.
T. Fontaine, C. Simenel, G. Dubreucq, O. Adam, M. Delepierre, J. Lemoine, C. E. Vorgias, M. Diaquin, and J.-P. Latge (2000)
J. Biol. Chem. 275, 27594-27607
   Abstract »    Full Text »    PDF »
The Yeast Cell Wall and Septum as Paradigms of Cell Growth and Morphogenesis.
E. Cabib, D.-H. Roh, M. Schmidt, L. B. Crotti, and A. Varma (2001)
J. Biol. Chem. 276, 19679-19682
   Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)