Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 5 April 1996:
Vol. 272. no. 5258, pp. 120 - 122
DOI: 10.1126/science.272.5258.120
Prev | Table of Contents | Next

Reports

Role of the Nuclear Transport Factor p10 in Nuclear Import

Ulf Nehrbass and Günter Blobel *

The nuclear import factor p10 was cloned from Saccharomyces cerevisiae and found to be essential. The protein p10 can bind directly to several peptide repeat-containing nucleoporins. It also binds to the guanosine triphosphatase (GTPase) Ran in its guanosine diphosphate (GDP)-bound form and to karyopherin beta . Assembly of the karyopherin heterodimer on immobilized nucleoporin yielded cooperative binding of p10 and Ran-GDP. Addition of GTP to this pentameric complex led to dissociation of karyopherin alpha , presumably via in situ formation of Ran-GTP from Ran-GDP. Thus, p10 appears to coordinate the Ran-dependent association and dissociation reactions underlying nuclear import.

Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021, USA.
* To whom correspondence should be addressed. E-mail: blobel{at}rockvax.rockefeller.edu


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Importin-{beta} Is a GDP-to-GTP Exchange Factor of Ran: IMPLICATIONS FOR THE MECHANISM OF NUCLEAR IMPORT.
T. G. Lonhienne, J. K. Forwood, M. Marfori, G. Robin, B. Kobe, and B. J. Carroll (2009)
J. Biol. Chem. 284, 22549-22558
   Abstract »    Full Text »    PDF »
The wild-type Ras: road ahead.
A. Singh, A. P. Sowjanya, and G. Ramakrishna (2005)
FASEB J 19, 161-169
   Abstract »    Full Text »    PDF »
The Docking of Kinesins, KIF5B and KIF5C, to Ran-binding Protein 2 (RanBP2) Is Mediated via a Novel RanBP2 Domain.
Y. Cai, B. B. Singh, A. Aslanukov, H. Zhao, and P. A. Ferreira (2001)
J. Biol. Chem. 276, 41594-41602
   Abstract »    Full Text »    PDF »
Nup2p Dynamically Associates with the Distal Regions of the Yeast Nuclear Pore Complex.
D. J. Dilworth, A. Suprapto, J. C. Padovan, B. T. Chait, R. W. Wozniak, M. P. Rout, and J. D. Aitchison (2001)
J. Cell Biol. 153, 1465-1478
   Abstract »    Full Text »    PDF »
The Yeast Nucleoporin Nup53p Specifically Interacts with Nic96p and Is Directly Involved in Nuclear Protein Import.
B. Fahrenkrog, W. Hübner, A. Mandinova, N. Panté, W. Keller, and U. Aebi (2000)
Mol. Biol. Cell 11, 3885-3896
   Abstract »    Full Text »
Selective Disruption of Nuclear Import by a Functional Mutant Nuclear Transport Carrier.
C. M. Lane, I. Cushman, and M. S. Moore (2000)
J. Cell Biol. 151, 321-332
   Abstract »    Full Text »    PDF »
Low-Dose Whole-Body Irradiation (LD-WBI) Changes Protein Expression of Mouse Thymocytes: Effect of a LD-WBI-Enhanced Protein RIP10 on Cell Proliferation and Spontaneous or Radiation-Induced Thymocyte Apoptosis.
S. L. Chen, L. Cai, Q. Y. Meng, S. Xu, H. Wan, and S. Z. Liu (2000)
Toxicol. Sci. 55, 97-106
   Abstract »    Full Text »    PDF »
The Dominant Negative Ras Mutant, N17Ras, Can Inhibit Signaling Independently of Blocking Ras Activation.
S. Stewart and K.-L. Guan (2000)
J. Biol. Chem. 275, 8854-8862
   Abstract »    Full Text »    PDF »
Dissecting the Interactions between NTF2, RanGDP, and the Nucleoporin XFXFG Repeats.
C. Chaillan-Huntington, C. V. Braslavsky, J. Kuhlmann, and M. Stewart (2000)
J. Biol. Chem. 275, 5874-5879
   Abstract »    Full Text »    PDF »
Monoclonal Antibodies to NTF2 Inhibit Nuclear Protein Import by Preventing Nuclear Translocation of the GTPase Ran.
S. M. Steggerda, B. E. Black, and B. M. Paschal (2000)
Mol. Biol. Cell 11, 703-719
   Abstract »    Full Text »
Identification of an NTF2-Related Factor That Binds Ran-GTP and Regulates Nuclear Protein Export.
B. E. Black, L. Levesque, J. M. Holaska, T. C. Wood, and B. M. Paschal (1999)
Mol. Cell. Biol. 19, 8616-8624
   Abstract »    Full Text »    PDF »
Nuclear Import of Sterol Regulatory Element-binding Protein-2, a Basic Helix-Loop-Helix-Leucine Zipper (bHLH-Zip)-containing Transcription Factor, Occurs through the Direct Interaction of Importin beta with HLH-Zip.
E. Nagoshi, N. Imamoto, R. Sato, and Y. Yoneda (1999)
Mol. Biol. Cell 10, 2221-2233
   Abstract »    Full Text »
Putative Reaction Intermediates in Crm1-mediated Nuclear Protein Export.
M. Floer and G. Blobel (1999)
J. Biol. Chem. 274, 16279-16286
   Abstract »    Full Text »    PDF »
Efficiency of Importin alpha /beta -Mediated Nuclear Localization Sequence Recognition and Nuclear Import. DIFFERENTIAL ROLE OF NTF2.
W. Hu and D. A. Jans (1999)
J. Biol. Chem. 274, 15820-15827
   Abstract »    Full Text »    PDF »
Characterization of Ikappa Balpha Nuclear Import Pathway.
P. Turpin, R. T. Hay, and C. Dargemont (1999)
J. Biol. Chem. 274, 6804-6812
   Abstract »    Full Text »    PDF »
Nuclear pore localization and nucleocytoplasmic transport of eIF-5A: evidence for direct interaction with the export receptor CRM1.
O Rosorius, B Reichart, F Kratzer, P Heger, M. Dabauvalle, and J Hauber (1999)
J. Cell Sci. 112, 2369-2380
   Abstract »    PDF »
Antagonistic effects of NES and NLS motifs determine S. cerevisiae Rna1p subcellular distribution.
W Feng, A. Benko, J. Lee, D. Stanford, and A. Hopper (1999)
J. Cell Sci. 112, 339-347
   Abstract »    PDF »
Specific Binding of the Karyopherin Kap121p to a Subunit of the Nuclear Pore Complex Containing Nup53p, Nup59p, and Nup170p.
M. Marelli, J. D. Aitchison, and R. W. Wozniak (1998)
J. Cell Biol. 143, 1813-1830
   Abstract »    Full Text »    PDF »
A protein required for nuclear-protein import, Mog1p, directly interacts with GTP-Gsp1p, the Saccharomyces cerevisiae Ran homologue.
M. Oki and T. Nishimoto (1998)
PNAS 95, 15388-15393
   Abstract »    Full Text »    PDF »
A cytosolic activity distinct from Crm1 mediates nuclear export of protein kinase inhibitor in permeabilized cells.
J. M. Holaska and B. M. Paschal (1998)
PNAS 95, 14739-14744
   Abstract »    Full Text »    PDF »
When Overexpressed, a Novel Centrosomal Protein, RanBPM, Causes Ectopic Microtubule Nucleation Similar to {gamma}-Tubulin.
M. Nakamura, H. Masuda, J. Horii, K.-i. Kuma, N. Yokoyama, T. Ohba, H. Nishitani, T. Miyata, M. Tanaka, and T. Nishimoto (1998)
J. Cell Biol. 143, 1041-1052
   Abstract »    Full Text »    PDF »
Determination of the Functional Domain Organization of the Importin {alpha} Nuclear Import Factor.
A. Herold, R. Truant, H. Wiegand, and B. R. Cullen (1998)
J. Cell Biol. 143, 309-318
   Abstract »    Full Text »    PDF »
The Cyclophilin-like Domain Mediates the Association of Ran-Binding Protein 2 with Subunits of the 19 S Regulatory Complex of the Proteasome.
P. A. Ferreira, C. Yunfei, D. Schick, and R. Roepman (1998)
J. Biol. Chem. 273, 24676-24682
   Abstract »    Full Text »    PDF »
Ran and Nuclear Transport.
M. S. Moore (1998)
J. Biol. Chem. 273, 22857-22860
   Full Text »    PDF »
Reconstitution of HIV-1 Rev nuclear export: Independent requirements for nuclear import and export.
D. C. Love, T. D. Sweitzer, and J. A. Hanover (1998)
PNAS 95, 10608-10613
   Abstract »    Full Text »    PDF »
Identification and Functional Characterization of a Novel Nuclear Localization Signal Present in the Yeast Nab2 Poly(A)+ RNA Binding Protein.
R. Truant, R. A. Fridell, R. E. Benson, H. Bogerd, and B. R. Cullen (1998)
Mol. Cell. Biol. 18, 1449-1458
   Abstract »    Full Text »
SUMO-1 Modification and Its Role in Targeting the Ran GTPase-activating Protein, RanGAP1, to the Nuclear Pore Complex.
M. J. Matunis, J. Wu, and G. Blobel (1998)
J. Cell Biol. 140, 499-509
   Abstract »    Full Text »    PDF »
The role of the ran GTPase in nuclear assembly and DNA replication: characterisation of the effects of Ran mutants.
M Hughes, C Zhang, J. Avis, C. Hutchison, and P. Clarke (1998)
J. Cell Sci. 111, 3017-3026
   Abstract »    PDF »
The nuclear import factor p10 regulates the functional size of the nuclear pore complex during oogenesis.
C Feldherr, D Akin, and M. Moore (1998)
J. Cell Sci. 111, 1889-1896
   Abstract »    PDF »
A Distinct and Parallel Pathway for the Nuclear Import of an mRNA-binding Protein.
L. F. Pemberton, J. S. Rosenblum, and G. Blobel (1997)
J. Cell Biol. 139, 1645-1653
   Abstract »    Full Text »    PDF »
A Nuclear Import Pathway for a Protein Involved in tRNA Maturation.
J. S. Rosenblum, L. F. Pemberton, and G. Blobel (1997)
J. Cell Biol. 139, 1655-1661
   Abstract »    Full Text »    PDF »
Yrb2p Is a Nuclear Protein That Interacts with Prp20p, a Yeast Rcc1 Homologue.
T. Taura, G. Schlenstedt, and P. A. Silver (1997)
J. Biol. Chem. 272, 31877-31884
   Abstract »    Full Text »    PDF »
RanGTP Targets p97 to RanBP2, a Filamentous Protein Localized at the Cytoplasmic Periphery of the Nuclear Pore Complex.
C. Delphin, T. Guan, F. Melchior, and L. Gerace (1997)
Mol. Biol. Cell 8, 2379-2390
   Abstract »    Full Text »
A T42A Ran Mutation: Differential Interactions with Effectors and Regulators, and Defect in Nuclear Protein Import.
G. A. Murphy, M. S. Moore, G. Drivas, P. P. de la Ossa, A. Villamarin, P. D'Eustachio, and M. G. Rush (1997)
Mol. Biol. Cell 8, 2591-2604
   Abstract »    Full Text »
Nucleocytoplasmic Recycling of the Nuclear Localization Signal Receptor {alpha} Subunit In Vivo Is Dependent on a Nuclear Export Signal, Energy, and RCC1.
I. Boche and E. Fanning (1997)
J. Cell Biol. 139, 313-325
   Abstract »    Full Text »    PDF »
Evidence for a Role of CRM1 in Signal-Mediated Nuclear Protein Export.
B. Ossareh-Nazari, F. Bachelerie, and C. Dargemont (1997)
Science 278, 141-144
   Abstract »    Full Text »
The Balance of RanBP1 and RCC1 Is Critical for Nuclear Assembly and Nuclear Transport.
R. T. Pu and M. Dasso (1997)
Mol. Biol. Cell 8, 1955-1970
   Abstract »    Full Text »
The Acidic C-terminal Domain of rna1p Is Required for the Binding of Ran·GTP and for RanGAP Activity.
J. Haberland, J. Becker, and V. Gerke (1997)
J. Biol. Chem. 272, 24717-24726
   Abstract »    Full Text »    PDF »
RanGTP-mediated nuclear export of karyopherin alpha  involves its interaction with the nucleoporin Nup153.
J. Moroianu, G. Blobel, and A. Radu (1997)
PNAS 94, 9699-9704
   Abstract »    Full Text »    PDF »
Kinetic Characterization of the Human Retinoblastoma Protein Bipartite Nuclear Localization Sequence (NLS) in Vivo and in Vitro. A COMPARISON WITH THE SV40 LARGE T-ANTIGEN NLS.
A. Efthymiadis, H. Shao, S. Hubner, and D. A. Jans (1997)
J. Biol. Chem. 272, 22134-22139
   Abstract »    Full Text »    PDF »
SV40 Large Tumor Antigen Nuclear Import Is Regulated by the Double-stranded DNA-dependent Protein Kinase Site (Serine 120) Flanking the Nuclear Localization Sequence.
C.-Y. Xiao, S. Hubner, and D. A. Jans (1997)
J. Biol. Chem. 272, 22191-22198
   Abstract »    Full Text »    PDF »
High Levels of the GTPase Ran/TC4 Relieve the Requirement for Nuclear Protein Transport Factor 2.
B. M. Paschal, C. Fritze, T. Guan, and L. Gerace (1997)
J. Biol. Chem. 272, 21534-21539
   Abstract »    Full Text »    PDF »
Disassembly of RanGTP-Karyopherin beta  Complex, an Intermediate in Nuclear Protein Import.
M. Floer, G. Blobel, and M. Rexach (1997)
J. Biol. Chem. 272, 19538-19546
   Abstract »    Full Text »    PDF »
Nup84, A Novel Nucleoporin That Is Associated With CAN/Nup214 on the Cytoplasmic Face of the Nuclear Pore Complex.
R. Bastos, L. R. d. Pouplana, M. Enarson, K. Bodoor, and B. Burke (1997)
J. Cell Biol. 137, 989-1000
   Abstract »    Full Text »    PDF »
A Nuclear Export Signal in Kap95p Is Required for Both Recycling the Import Factor and Interaction with the Nucleoporin GLFG Repeat Regions of Nup116p and Nup100p.
M. K. Iovine and S. R. Wente (1997)
J. Cell Biol. 137, 797-811
   Abstract »    Full Text »    PDF »
Karyopherin beta 2 mediates nuclear import of a mRNA binding protein.
N. Bonifaci, J. Moroianu, A. Radu, and G. Blobel (1997)
PNAS 94, 5055-5060
   Abstract »    Full Text »    PDF »
Subnuclear Trafficking of Glucocorticoid Receptors In Vitro: Chromatin Recycling and Nuclear Export.
J. Yang, J. Liu, and D. B. DeFranco (1997)
J. Cell Biol. 137, 523-538
   Abstract »    Full Text »    PDF »
Cloning and characterization of human karyopherin beta 3.
N. R. Yaseen and G. Blobel (1997)
PNAS 94, 4451-4456
   Abstract »    Full Text »    PDF »
Two active states of the Ras-related Bud1/Rsr1 protein bind to different effectors to determine yeast cell polarity.
H.-O. Park, E. Bi, J. R. Pringle, and I. Herskowitz (1997)
PNAS 94, 4463-4468
   Abstract »    Full Text »    PDF »
The Vertebrate GLFG Nucleoporin, Nup98, Is an Essential Component of Multiple RNA Export Pathways.
M. A. Powers, D. J. Forbes, J. E. Dahlberg, and E. Lund (1997)
J. Cell Biol. 136, 241-250
   Abstract »    Full Text »    PDF »
Xenopus Ran-binding protein 1: molecular interactions and effects on nuclear assembly in Xenopus egg extracts.
F. Nicolas, C Zhang, M Hughes, M Goldberg, S Watton, and P Clarke (1997)
J. Cell Sci. 110, 3019-3030
   Abstract »    PDF »
Nuclear import of hnRNP A1 is mediated by a novel cellular cofactor related to karyopherin-beta.
R. Fridell, R Truant, L Thorne, R. Benson, and B. Cullen (1997)
J. Cell Sci. 110, 1325-1331
   Abstract »    PDF »
Mutations within the Ran/TC4 GTPase. EFFECTS ON REGULATORY FACTOR INTERACTIONS AND SUBCELLULAR LOCALIZATION.
K. M. Lounsbury, S. A. Richards, K. L. Carey, and I. G. Macara (1996)
J. Biol. Chem. 271, 32834-32841
   Abstract »    Full Text »    PDF »
Expression in yeast of binding regions of karyopherins alpha  and beta  inhibits nuclear import and cell growth.
C. Enenkel, N. Schulke, and G. Blobel (1996)
PNAS 93, 12986-12991
   Abstract »    Full Text »    PDF »
Kap104p: A Karyopherin Involved in the Nuclear Transport of Messenger RNA Binding Proteins.
J. D. Aitchison, G. Blobel, and M. P. Rout (1996)
Science 274, 624-627
   Abstract »    Full Text »
Sequential Binding of Import Ligands to Distinct Nucleopore Regions During Their Nuclear Import.
N. Panté and U. Aebi (1996)
Science 273, 1729-1732
   Abstract »    Full Text »
The NTF2 Gene Encodes an Essential, Highly Conserved Protein That Functions in Nuclear Transport in Vivo.
A. H. Corbett and P. A. Silver (1996)
J. Biol. Chem. 271, 18477-18484
   Abstract »    Full Text »    PDF »
Ran, a GTPase involved in nuclear processes: its regulators and effectors.
J. Avis and P. Clarke (1996)
J. Cell Sci. 109, 2423-2427
   Abstract »    PDF »
Nuclear Import of Insulin-like Growth Factor-binding Protein-3 and -5 Is Mediated by the Importin beta Subunit.
L. J. Schedlich, S. L. Le Page, S. M. Firth, L. J. Briggs, D. A. Jans, and R. C. Baxter (2000)
J. Biol. Chem. 275, 23462-23470
   Abstract »    Full Text »    PDF »
Ikappa Balpha and Ikappa Balpha /NF-kappa B Complexes Are Retained in the Cytoplasm through Interaction with a Novel Partner, RasGAP SH3-binding Protein 2.
M. Prigent, I. Barlat, H. Langen, and C. Dargemont (2000)
J. Biol. Chem. 275, 36441-36449
   Abstract »    Full Text »    PDF »
The Mechanism of Ran Import into the Nucleus by Nuclear Transport Factor 2.
B. B. Quimby, T. Lamitina, S. W. L'Hernault, and A. H. Corbett (2000)
J. Biol. Chem. 275, 28575-28582
   Abstract »    Full Text »    PDF »
Proteomic Analysis of Nucleoporin Interacting Proteins.
N. P. C. Allen, L. Huang, A. Burlingame, and M. Rexach (2001)
J. Biol. Chem. 276, 29268-29274
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)