Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 22 March 1996:
Vol. 271. no. 5256, pp. 1744 - 1747
DOI: 10.1126/science.271.5256.1744
Prev | Table of Contents

Reports

Abnormal Centrosome Amplification in the Absence of p53

Kenji Fukasawa, Taesaeng Choi, Ryoko Kuriyama, Shen Rulong, George F. Vande Woude *

The centrosome plays a vital role in mitotic fidelity, ensuring establishment of bipolar spindles and balanced chromosome segregation. Centrosome duplication occurs only once during the cell cycle and is therefore highly regulated. Here, it is shown that in mouse embryonic fibroblasts (MEFs) lacking the p53 tumor suppressor protein, multiple copies of functionally competent centrosomes are generated during a single cell cycle. In contrast, MEFs prepared from normal mice or mice deficient in the retinoblastoma tumor suppressor gene product do not display these abnormalities. The abnormally amplified centrosomes profoundly affect mitotic fidelity, resulting in unequal segregation of chromosomes. These observations implicate p53 in the regulation of centrosome duplication and suggest one possible mechanism by which the loss of p53 may cause genetic instability.

K. Fukasawa, T. Choi, S. Rulong, G. F. Vande Woude, ABL-Basic Research Program, National Cancer Institute, Frederick Cancer Research and Development Center, Post Office Box B, Frederick, MD 21702-1201, USA.
R. Kuriyama, Department of Cell Biology and Neuroanatomy, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
* To whom correspondence should be adressed.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cdk2 and Cdk4 Regulate the Centrosome Cycle and Are Critical Mediators of Centrosome Amplification in p53-Null Cells.
A. M. Adon, X. Zeng, M. K. Harrison, S. Sannem, H. Kiyokawa, P. Kaldis, and H. I. Saavedra (2010)
Mol. Cell. Biol. 30, 694-710
   Abstract »    Full Text »    PDF »
Wild-Type p53 Enhances Efficiency of Simian Virus 40 Large-T-Antigen-Induced Cellular Transformation.
A. Hermannstadter, C. Ziegler, M. Kuhl, W. Deppert, and G. V. Tolstonog (2009)
J. Virol. 83, 10106-10118
   Abstract »    Full Text »    PDF »
BRCA1 Interaction of Centrosomal Protein Nlp Is Required for Successful Mitotic Progression.
S. Jin, H. Gao, L. Mazzacurati, Y. Wang, W. Fan, Q. Chen, W. Yu, M. Wang, X. Zhu, C. Zhang, et al. (2009)
J. Biol. Chem. 284, 22970-22977
   Abstract »    Full Text »    PDF »
Spontaneous Tumor Formation in Trp53-deficient Epidermis Mediated by Chromosomal Instability and Inflammation.
A. B. MARTINEZ-CRUZ, M. SANTOS, R. GARCIA-ESCUDERO, M. MORAL, C. SEGRELLES, C. LORZ, C. SAIZ, A. BUITRAGO-PEREZ, C. COSTA, and J. M. PARAMIO (2009)
Anticancer Res 29, 3035-3042
   Abstract »    Full Text »    PDF »
{gamma}-Tubulin-containing abnormal centrioles are induced by insufficient Plk4 in human HCT116 colorectal cancer cells.
R. Kuriyama, M. Bettencourt-Dias, I. Hoffmann, M. Arnold, and L. Sandvig (2009)
J. Cell Sci. 122, 2014-2023
   Abstract »    Full Text »    PDF »
DNA damage induced p53 downregulates Cdc20 by direct binding to its promoter causing chromatin remodeling.
T. Banerjee, S. Nath, and S. Roychoudhury (2009)
Nucleic Acids Res. 37, 2688-2698
   Abstract »    Full Text »    PDF »
Loss of p53 and MCT-1 Overexpression Synergistically Promote Chromosome Instability and Tumorigenicity.
R. Kasiappan, H.-J. Shih, K.-L. Chu, W.-T. Chen, H.-P. Liu, S.-F. Huang, C. O. Choy, C.-L. Shu, R. Din, J.-S. Chu, et al. (2009)
Mol. Cancer Res. 7, 536-548
   Abstract »    Full Text »    PDF »
Phosphorylation of p53 Is Regulated by TPX2-Aurora A in Xenopus Oocytes.
G. Pascreau, F. Eckerdt, A. L. Lewellyn, C. Prigent, and J. L. Maller (2009)
J. Biol. Chem. 284, 5497-5505
   Abstract »    Full Text »    PDF »
MdmX Promotes Bipolar Mitosis To Suppress Transformation and Tumorigenesis in p53-Deficient Cells and Mice.
Z. Matijasevic, H. A. Steinman, K. Hoover, and S. N. Jones (2008)
Mol. Cell. Biol. 28, 1265-1273
   Abstract »    Full Text »    PDF »
Hbo1 Links p53-Dependent Stress Signaling to DNA Replication Licensing.
M. Iizuka, O. F. Sarmento, T. Sekiya, H. Scrable, C. D. Allis, and M. M. Smith (2008)
Mol. Cell. Biol. 28, 140-153
   Abstract »    Full Text »    PDF »
Somatic loss of BRCA1 and p53 in mice induces mammary tumors with features of human BRCA1-mutated basal-like breast cancer.
X. Liu, H. Holstege, H. van der Gulden, M. Treur-Mulder, J. Zevenhoven, A. Velds, R. M. Kerkhoven, M. H. van Vliet, L. F. A. Wessels, J. L. Peterse, et al. (2007)
PNAS 104, 12111-12116
   Abstract »    Full Text »    PDF »
Physical and functional interaction between mortalin and Mps1 kinase.
M. Kanai, Z. Ma, H. Izumi, S.-H. Kim, C. P. Mattison, M. Winey, and K. Fukasawa (2007)
Genes Cells 12, 797-810
   Abstract »    Full Text »    PDF »
Telomere-Driven Karyotypic Complexity Concurs with p16INK4a Inactivation in TP53-Competent Immortal Endothelial Cells..
V. W. Wen, K. Wu, S. Baksh, R. A. Hinshelwood, R. B. Lock, S. J. Clark, M. A.S. Moore, and K. L. MacKenzie (2006)
Cancer Res. 66, 10691-10700
   Abstract »    Full Text »    PDF »
A positive feedback loop between the p53 and Lats2 tumor suppressors prevents tetraploidization.
Y. Aylon, D. Michael, A. Shmueli, N. Yabuta, H. Nojima, and M. Oren (2006)
Genes & Dev. 20, 2687-2700
   Abstract »    Full Text »    PDF »
Gadd45a Interacts with Aurora-A and Inhibits Its Kinase Activity.
S. Shao, Y. Wang, S. Jin, Y. Song, X. Wang, W. Fan, Z. Zhao, M. Fu, T. Tong, L. Dong, et al. (2006)
J. Biol. Chem. 281, 28943-28950
   Abstract »    Full Text »    PDF »
Cytogenetic instability in ovarian epithelial cells from women at risk of ovarian cancer..
T. Pejovic, J. E. Yates, H. Y. Liu, L. E. Hays, Y. Akkari, Y. Torimaru, W. Keeble, R. K. Rathbun, W. H. Rodgers, A. E. Bale, et al. (2006)
Cancer Res. 66, 9017-9025
   Abstract »    Full Text »    PDF »
Haploinsufficiency of RAD51B Causes Centrosome Fragmentation and Aneuploidy in Human Cells..
O. Date, M. Katsura, M. Ishida, T. Yoshihara, A. Kinomura, T. Sueda, and K. Miyagawa (2006)
Cancer Res. 66, 6018-6024
   Abstract »    Full Text »    PDF »
Biological characteristics in bladder cancer depend on the type of genetic instability..
Y. Yamamoto, H. Matsuyama, S. Kawauchi, T. Furuya, X. P. Liu, K. Ikemoto, A. Oga, K. Naito, and K. Sasaki (2006)
Clin. Cancer Res. 12, 2752-2758
   Abstract »    Full Text »    PDF »
Suppression of Centrosome Amplification after DNA Damage Depends on p27 Accumulation..
E. Sugihara, M. Kanai, S. Saito, T. Nitta, H. Toyoshima, K. Nakayama, K. I. Nakayama, K. Fukasawa, M. Schwab, H. Saya, et al. (2006)
Cancer Res. 66, 4020-4029
   Abstract »    Full Text »    PDF »
Pin1 Regulates Centrosome Duplication, and Its Overexpression Induces Centrosome Amplification, Chromosome Instability, and Oncogenesis.
F. Suizu, A. Ryo, G. Wulf, J. Lim, and K. P. Lu (2006)
Mol. Cell. Biol. 26, 1463-1479
   Abstract »    Full Text »    PDF »
Induction of Centrosome Amplification during Arsenite-Induced Mitotic Arrest in CGL-2 Cells.
L.-H. Yih, Y.-Y. Tseng, Y.-C. Wu, and T.-C. Lee (2006)
Cancer Res. 66, 2098-2106
   Abstract »    Full Text »    PDF »
Hzf, a p53-Responsive Gene, Regulates Maintenance of the G2 Phase Checkpoint Induced by DNA Damage.
M. Sugimoto, A. Gromley, and C. J. Sherr (2006)
Mol. Cell. Biol. 26, 502-512
   Abstract »    Full Text »    PDF »
Tumour proliferation, angiogenesis, and ploidy status in human colon cancer.
E Cristi, G Perrone, G Toscano, A Verzi, S Nori, D Santini, G Tonini, A Vetrani, A Fabiano, and C Rabitti (2005)
J. Clin. Pathol. 58, 1170-1174
   Abstract »    Full Text »    PDF »
Transcriptional Control of BubR1 by p53 and Suppression of Centrosome Amplification by BubR1.
T. Oikawa, M. Okuda, Z. Ma, R. Goorha, H. Tsujimoto, H. Inokuma, and K. Fukasawa (2005)
Mol. Cell. Biol. 25, 4046-4061
   Abstract »    Full Text »    PDF »
Centrosomal P4.1-associated Protein Is a New Member of Transcriptional Coactivators for Nuclear Factor-{kappa}B.
M. Koyanagi, M. Hijikata, K. Watashi, O. Masui, and K. Shimotohno (2005)
J. Biol. Chem. 280, 12430-12437
   Abstract »    Full Text »    PDF »
Defective sister-chromatid cohesion, aneuploidy and cancer predisposition in a mouse model of type II Rothmund-Thomson syndrome.
M. B. Mann, C. A. Hodges, E. Barnes, H. Vogel, T. J. Hassold, and G. Luo (2005)
Hum. Mol. Genet. 14, 813-825
   Abstract »    Full Text »    PDF »
Translocation of XRCC1 and DNA ligase III{alpha} from centrosomes to chromosomes in response to DNA damage in mitotic human cells.
S. Okano, L. Lan, A. E. Tomkinson, and A. Yasui (2005)
Nucleic Acids Res. 33, 422-429
   Abstract »    Full Text »    PDF »
Oncogene-dependent Tumor Suppression: Using the Dark Side of the Force for Cancer Therapy.
G.I. EVAN, M. CHRISTOPHOROU, E.A. LAWLOR, I. RINGSHAUSEN, J. PRESCOTT, T. DANSEN, A. FINCH, C. MARTINS, and D. MURPHY (2005)
Cold Spring Harb Symp Quant Biol 70, 263-273
   Abstract »    PDF »
Centrosome-targeting region of CG-NAP causes centrosome amplification by recruiting cyclin E-cdk2 complex.
T. Nishimura, M. Takahashi, H.-S. Kim, H. Mukai, and Y. Ono (2005)
Genes Cells 10, 75-86
   Abstract »    Full Text »    PDF »
Seckel syndrome exhibits cellular features demonstrating defects in the ATR-signalling pathway.
G. K. Alderton, H. Joenje, R. Varon, A. D. Borglum, P. A. Jeggo, and M. O'Driscoll (2004)
Hum. Mol. Genet. 13, 3127-3138
   Abstract »    Full Text »    PDF »
Aurora-A Abrogation of p53 DNA Binding and Transactivation Activity by Phosphorylation of Serine 215.
Q. Liu, S. Kaneko, L. Yang, R. I. Feldman, S. V. Nicosia, J. Chen, and J. Q. Cheng (2004)
J. Biol. Chem. 279, 52175-52182
   Abstract »    Full Text »    PDF »
The Sac3 Homologue shd1 Is Involved in Mitotic Progression in Mammalian Cells.
S.-e- Khuda, M. Yoshida, Y. Xing, T. Shimasaki, M. Takeya, K. Kuwahara, and N. Sakaguchi (2004)
J. Biol. Chem. 279, 46182-46190
   Abstract »    Full Text »    PDF »
BRCA1-Dependent Ubiquitination of {gamma}-Tubulin Regulates Centrosome Number.
L. M. Starita, Y. Machida, S. Sankaran, J. E. Elias, K. Griffin, B. P. Schlegel, S. P. Gygi, and J. D. Parvin (2004)
Mol. Cell. Biol. 24, 8457-8466
   Abstract »    Full Text »    PDF »
Stat3 Activity Is Required for Centrosome Duplication in Chinese Hamster Ovary Cells.
B. Metge, S. Ofori-Acquah, T. Stevens, and R. Balczon (2004)
J. Biol. Chem. 279, 41801-41806
   Abstract »    Full Text »    PDF »
Centrosome Hyperamplification Predicts Progression and Tumor Recurrence in Bladder Cancer.
Y. Yamamoto, H. Matsuyama, T. Furuya, A. Oga, S. Yoshihiro, M. Okuda, S. Kawauchi, K. Sasaki, and K. Naito (2004)
Clin. Cancer Res. 10, 6449-6455
   Abstract »    Full Text »    PDF »
Function of Drg1/Rit42 in p53-dependent Mitotic Spindle Checkpoint.
K.-t. Kim, P. P. Ongusaha, Y.-K. Hong, S. K. Kurdistani, M. Nakamura, K. P. Lu, and S. W. Lee (2004)
J. Biol. Chem. 279, 38597-38602
   Abstract »    Full Text »    PDF »
Hormone-Induced Chromosomal Instability in p53-Null Mammary Epithelium.
D. Pati, B. R. Haddad, A. Haegele, H. Thompson, F. S. Kittrell, A. Shepard, C. Montagna, N. Zhang, G. Ge, S. K. Otta, et al. (2004)
Cancer Res. 64, 5608-5616
   Abstract »    Full Text »    PDF »
Noscapine Crosses the Blood-Brain Barrier and Inhibits Glioblastoma Growth.
J. W. Landen, V. Hau, M. Wang, T. Davis, B. Ciliax, B. H. Wainer, E. G. Van Meir, J. D. Glass, H. C. Joshi, and D. R. Archer (2004)
Clin. Cancer Res. 10, 5187-5201
   Abstract »    Full Text »    PDF »
Induction of Centrosome Amplification and Chromosome Instability in Human Bladder Cancer Cells by p53 Mutation and Cyclin E Overexpression.
K. Kawamura, H. Izumi, Z. Ma, R. Ikeda, M. Moriyama, T. Tanaka, T. Nojima, L. S. Levin, K. Fujikawa-Yamamoto, K. Suzuki, et al. (2004)
Cancer Res. 64, 4800-4809
   Abstract »    Full Text »    PDF »
Transmission of {gamma}-ray-induced unstable chromosomal aberrations through successive mitotic divisions in human lymphocytes in vitro.
A.P. Krishnaja and N.K. Sharma (2004)
Mutagenesis 19, 299-305
   Abstract »    Full Text »    PDF »
Eukaryotic Cells and their Cell Bodies: Cell Theory Revised.
F. BALUSKA, D. VOLKMANN, and P. W. BARLOW (2004)
Ann. Bot. 94, 9-32
   Abstract »    Full Text »    PDF »
Overexpression of cyclin E protein is associated with specific mutation types in the p53 gene and poor survival in human breast cancer.
T. Lindahl, G. Landberg, J. Ahlgren, H. Nordgren, T. Norberg, S. Klaar, L. Holmberg, and J. Bergh (2004)
Carcinogenesis 25, 375-380
   Abstract »    Full Text »    PDF »
Survivin Loss in Thymocytes Triggers p53-mediated Growth Arrest and p53-independent Cell Death.
H. Okada, C. Bakal, A. Shahinian, A. Elia, A. Wakeham, W.-K. Suh, G. S. Duncan, M. Ciofani, R. Rottapel, J. C. Zuniga-Pflucker, et al. (2004)
J. Exp. Med. 199, 399-410
   Abstract »    Full Text »    PDF »
E6 and E7 Oncoproteins Induce Distinct Patterns of Chromosomal Aneuploidy in Skin Tumors from Transgenic Mice.
A. J. Schaeffer, M. Nguyen, A. Liem, D. Lee, C. Montagna, P. F. Lambert, T. Ried, and M. J. Difilippantonio (2004)
Cancer Res. 64, 538-546
   Abstract »    Full Text »    PDF »
REVIEW The restriction site mutation (RSM) method: clinical applications.
G.J.S. Jenkins (2004)
Mutagenesis 19, 3-11
   Abstract »    Full Text »    PDF »
Aneuploidy and Telomere Attrition Are Independent Determinants of Crisis in SV40-transformed Epithelial Cells.
M. Velicescu, J. Yu, B.-S. Herbert, J. W. Shay, E. Granada, and L. Dubeau (2003)
Cancer Res. 63, 5813-5820
   Abstract »    Full Text »    PDF »
Involvement of Crm1 in Hepatitis B Virus X Protein-Induced Aberrant Centriole Replication and Abnormal Mitotic Spindles.
M. Forgues, M. J. Difilippantonio, S. P. Linke, T. Ried, K. Nagashima, J. Feden, K. Valerie, K. Fukasawa, and X. W. Wang (2003)
Mol. Cell. Biol. 23, 5282-5292
   Abstract »    Full Text »    PDF »
Molecular Phenotype of Spontaneously Arising 4N (G2-Tetraploid) Intermediates of Neoplastic Progression in Barrett's Esophagus.
M. T. Barrett, D. Pritchard, C. Palanca-Wessels, J. Anderson, B. J. Reid, and P. S. Rabinovitch (2003)
Cancer Res. 63, 4211-4217
   Abstract »    Full Text »    PDF »
Drosophila skpA, a component of SCF ubiquitin ligases, regulates centrosome duplication independently of cyclin E accumulation.
T. D. Murphy (2003)
J. Cell Sci. 116, 2321-2332
   Abstract »    Full Text »    PDF »
Centrosomes Split in the Presence of Impaired DNA Integrity during Mitosis.
H. M.J. Hut, W. Lemstra, E. H. Blaauw, G. W.A. van Cappellen, H. H. Kampinga, and O. C.M. Sibon (2003)
Mol. Biol. Cell 14, 1993-2004
   Abstract »    Full Text »    PDF »
PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression.
A. Augustin, C. Spenlehauer, H. Dumond, J. Menissier-de Murcia, M. Piel, A.-C. Schmit, F. Apiou, J.-L. Vonesch, M. Kock, M. Bornens, et al. (2003)
J. Cell Sci. 116, 1551-1562
   Abstract »    Full Text »    PDF »
Involvement of Poly(ADP-Ribose) Polymerase 1 and Poly(ADP-Ribosyl)ation in Regulation of Centrosome Function.
M. Kanai, W.-M. Tong, E. Sugihara, Z.-Q. Wang, K. Fukasawa, and M. Miwa (2003)
Mol. Cell. Biol. 23, 2451-2462
   Abstract »    Full Text »    PDF »
Centrosome Abnormalities and Chromosome Instability Occur Together in Pre-invasive Carcinomas.
G. A. Pihan, J. Wallace, Y. Zhou, and S. J. Doxsey (2003)
Cancer Res. 63, 1398-1404
   Abstract »    Full Text »    PDF »
Mutations in Drosophila myb lead to centrosome amplification and genomic instability.
S.-M. Fung, G. Ramsay, and A. L. Katzen (2003)
Development 129, 347-359
   Abstract »    Full Text »    PDF »
Polo-like Kinase 1 and Chk2 Interact and Co-localize to Centrosomes and the Midbody.
L. Tsvetkov, X. Xu, J. Li, and D. F. Stern (2003)
J. Biol. Chem. 278, 8468-8475
   Abstract »    Full Text »    PDF »
The centrosomal kinase Aurora-A/STK15 interacts with a putative tumor suppressor NM23-H1.
J. Du and G. J. Hannon (2002)
Nucleic Acids Res. 30, 5465-5475
   Abstract »    Full Text »    PDF »
MBD3 and HDAC1, Two Components of the NuRD Complex, Are Localized at Aurora-A-positive Centrosomes in M Phase.
H. Sakai, T. Urano, K. Ookata, M.-H. Kim, Y. Hirai, M. Saito, Y. Nojima, and F. Ishikawa (2002)
J. Biol. Chem. 277, 48714-48723
   Abstract »    Full Text »    PDF »
In Vivo Interference with Skp1 Function Leads to Genetic Instability and Neoplastic Transformation.
R. Piva, J. Liu, R. Chiarle, A. Podda, M. Pagano, and G. Inghirami (2002)
Mol. Cell. Biol. 22, 8375-8387
   Abstract »    Full Text »    PDF »
Detection and characterization of mechanisms of action of aneugenic chemicals.
E.M. Parry, J.M. Parry, C. Corso, A. Doherty, F. Haddad, T.F. Hermine, G. Johnson, M. Kayani, E. Quick, T. Warr, et al. (2002)
Mutagenesis 17, 509-521
   Abstract »    Full Text »    PDF »
De novo formation of centrosomes in vertebrate cells arrested during S phase.
A. Khodjakov, C. L. Rieder, G. Sluder, G. Cassels, O. Sibon, and C.-L. Wang (2002)
J. Cell Biol. 158, 1171-1181
   Abstract »    Full Text »    PDF »
Accelerated Progression of Asbestos-Induced Mesotheliomas in Heterozygous p53+/- Mice.
C. A. Vaslet, N. J. Messier, and A. B. Kane (2002)
Toxicol. Sci. 68, 331-338
   Abstract »    Full Text »    PDF »
Multiple centrosomes arise from tetraploidy checkpoint failure and mitotic centrosome clusters in p53 and RB pocket protein-compromised cells.
F. Borel, O. D. Lohez, F. B. Lacroix, and R. L. Margolis (2002)
PNAS 99, 9819-9824
   Abstract »    Full Text »    PDF »
Induction of Gene Amplification as a Gain-of-Function Phenotype of Mutant p53 Proteins.
S. El-Hizawi, J. P. Lagowski, M. Kulesz-Martin, and A. Albor (2002)
Cancer Res. 62, 3264-3270
   Abstract »    Full Text »    PDF »
Embryonic stem cells and somatic cells differ in mutation frequency and type.
R. B. Cervantes, J. R. Stringer, C. Shao, J. A. Tischfield, and P. J. Stambrook (2002)
PNAS 99, 3586-3590
   Abstract »    Full Text »    PDF »
Cell-cycle-dependent Regulation of Human aurora A Transcription Is Mediated by Periodic Repression of E4TF1.
M. Tanaka, A. Ueda, H. Kanamori, H. Ideguchi, J. Yang, S. Kitajima, and Y. Ishigatsubo (2002)
J. Biol. Chem. 277, 10719-10726
   Abstract »    Full Text »    PDF »
Calcium, Calmodulin, and CaMKII Requirement for Initiation of Centrosome Duplication in Xenopus Egg Extracts.
Y. Matsumoto and J. L. Maller (2002)
Science 295, 499-502
   Abstract »    Full Text »    PDF »
Kinetochore localisation of the DNA damage response component 53BP1 during mitosis.
D. Jullien, P. Vagnarelli, W. C. Earnshaw, and Y. Adachi (2002)
J. Cell Sci. 115, 71-79
   Abstract »    Full Text »    PDF »
Micronuclei and p53 accumulation in preneoplastic and malignant lesions of the head and neck.
V. Delfino, G. Casartelli, B. Garzoglio, M. Scala, P. Mereu, S. Bonatti, G. Margarino, and A. Abbondandolo (2002)
Mutagenesis 17, 73-77
   Abstract »    Full Text »    PDF »
Persistent Increase in Chromosome Instability in Lung Cancer : Possible Indirect Involvement of p53 Inactivation.
N. Haruki, T. Harano, A. Masuda, T. Kiyono, T. Takahashi, Y. Tatematsu, S. Shimizu, T. Mitsudomi, H. Konishi, H. Osada, et al. (2001)
Am. J. Pathol. 159, 1345-1352
   Abstract »    Full Text »
Selective Inactivation of p53 Facilitates Mouse Epithelial Tumor Progression without Chromosomal Instability.
X. Lu, G. Magrane, C. Yin, D. N. Louis, J. Gray, and T. Van Dyke (2001)
Mol. Cell. Biol. 21, 6017-6030
   Abstract »    Full Text »    PDF »
"It Takes Two to Tango": understanding how centrosome duplication is regulated throughout the cell cycle.
E. H. Hinchcliffe and G. Sluder (2001)
Genes & Dev. 15, 1167-1181
   Full Text »
Frequent Inactivation of the TP53 Gene in Esophageal Squamous Cell Carcinoma from a High-Risk Population in China.
N. Hu, J. Huang, M. R. Emmert-Buck, Z.-Z. Tang, M. J. Roth, C. Wang, S. M. Dawsey, G. Li, W.-J. Li, Q.-H. Wang, et al. (2001)
Clin. Cancer Res. 7, 883-891
   Abstract »    Full Text »
Mutations With Loss of Heterozygosity of p53 Are Common in Therapy-Related Myelodysplasia and Acute Myeloid Leukemia After Exposure to Alkylating Agents and Significantly Associated With Deletion or Loss of 5q, a Complex Karyotype, and a Poor Prognosis.
D. H. Christiansen, M. K. Andersen, and J. Pedersen-Bjergaard (2001)
J. Clin. Oncol. 19, 1405-1413
   Abstract »    Full Text »    PDF »
Inhibition of poly(ADP-ribose) polymerase activity is insufficient to induce tetraploidy.
C. M. Simbulan-Rosenthal, D. S. Rosenthal, R. Luo, J.-H. Li, J. Zhang, and M. E. Smulson (2001)
Nucleic Acids Res. 29, 841-849
   Abstract »    Full Text »    PDF »
Kendrin/pericentrin-B, a centrosome protein with homology to pericentrin that complexes with PCM-1.
Q Li, D Hansen, A Killilea, H. Joshi, R. Palazzo, and R Balczon (2001)
J. Cell Sci. 114, 797-809
   Abstract »    PDF »
Heritable effects of paternal irradiation in mice on signaling protein kinase activities in F3 offspring.
J. E. Baulch, O. G. Raabe, and L. M. Wiley (2001)
Mutagenesis 16, 17-23
   Abstract »    Full Text »    PDF »
Progesterone facilitates chromosome instability (aneuploidy) in p53 null normal mammary epithelial cells.
T. M. GOEPFERT, M. MCCARTHY, F. S. KITTRELL, C. STEPHENS, R. L. ULLRICH, B. R. BRINKLEY, and D. MEDINA (2000)
FASEB J 14, 2221-2229
   Abstract »    Full Text »
A gain of function p53 mutant promotes both genomic instability and cell survival in a novel p53-null mammary epithelial cell model.
K. L. MURPHY, A. P. DENNIS, and J. M. ROSEN (2000)
FASEB J 14, 2291-2302
   Abstract »    Full Text »
Base excision repair is efficient in cells lacking poly(ADP-ribose) polymerase 1.
M. D. Vodenicharov, F. R. Sallmann, M. S. Satoh, and G. G. Poirier (2000)
Nucleic Acids Res. 28, 3887-3896
   Abstract »    Full Text »    PDF »
The human papillomavirus type 16 E6 and E7 oncoproteins cooperate to induce mitotic defects and genomic instability by uncoupling centrosome duplication from the cell division cycle.
S. Duensing, L. Y. Lee, A. Duensing, J. Basile, S.-o. Piboonniyom, S. Gonzalez, C. P. Crum, and K. Munger (2000)
PNAS
   Abstract »    Full Text »
Multicolour FISH detection of radioactive iodine-induced 17cen-p53 chromosomal breakage in buccal cells from therapeutically exposed patients.
M.J. Ramirez, S. Puerto, P. Galofre, E.M. Parry, J.M. Parry, A. Creus, R. Marcos, and J. Surralles (2000)
Carcinogenesis 21, 1581-1586
   Abstract »    Full Text »    PDF »
Genetic and Hormonal Risk Factors in Breast Cancer.
A.-M. Martin and B. L. Weber (2000)
J Natl Cancer Inst 92, 1126-1135
   Abstract »    Full Text »    PDF »
Chromosome instability contributes to loss of heterozygosity in mice lacking p53.
C. Shao, L. Deng, O. Henegariu, L. Liang, P. J. Stambrook, and J. A. Tischfield (2000)
PNAS 97, 7405-7410
   Abstract »    Full Text »    PDF »
Cancer Risk and the ATM Gene: a Continuing Debate.
K. K. Khanna (2000)
J Natl Cancer Inst 92, 795-802
   Abstract »    Full Text »    PDF »
Retinoblastoma Protein Enhances the Fidelity of Chromosome Segregation Mediated by hsHec1p.
L. Zheng, Y. Chen, D. J. Riley, P.-L. Chen, and W.-H. Lee (2000)
Mol. Cell. Biol. 20, 3529-3537
   Abstract »    Full Text »    PDF »
XCS-1, a maternally expressed gene product involved in regulating mitosis in Xenopus.
H Nakamura, C Wu, J Kuang, C Larabell, and L. Etkin (2000)
J. Cell Sci. 113, 2497-2505
   Abstract »    PDF »
Role of DNA Break-sensing Molecule Poly(ADP-ribose) Polymerase (PARP) in Cellular Function and Radiation Toxicity.
W.-M. TONG, D. GALENDO, and Z.-Q. WANG (2000)
Cold Spring Harb Symp Quant Biol 65, 583-592
   Abstract »    PDF »
MAPK Mediates RAS-induced Chromosome Instability.
H. I. Saavedra, K. Fukasawa, C. W. Conn, and P. J. Stambrook (1999)
J. Biol. Chem. 274, 38083-38090
   Abstract »    Full Text »    PDF »
Defects in transforming growth factor-beta signaling cooperate with a Ras oncogene to cause rapid aneuploidy and malignant transformation of mouse keratinocytes.
A. Glick, N. Popescu, V. Alexander, H. Ueno, E. Bottinger, and S. H. Yuspa (1999)
PNAS 96, 14949-14954
   Abstract »    Full Text »    PDF »
Altered Centrosome Structure Is Associated with Abnormal Mitoses in Human Breast Tumors.
W. L. Lingle and J. L. Salisbury (1999)
Am. J. Pathol. 155, 1941-1951
   Abstract »    Full Text »    PDF »
Chromosomal aberrations in PARP-/- mice: Genome stabilization in immortalized cells by reintroduction of poly(ADP-ribose) polymerase cDNA.
C. M. Simbulan-Rosenthal, B. R. Haddad, D. S. Rosenthal, Z. Weaver, A. Coleman, R. Luo, H. M. Young, Z.-Q. Wang, T. Ried, and M. E. Smulson (1999)
PNAS 96, 13191-13196
   Abstract »    Full Text »    PDF »
Disruption of the ARF-Mdm2-p53 tumor suppressor pathway in Myc-induced lymphomagenesis.
C. M. Eischen, J. D. Weber, M. F. Roussel, C. J. Sherr, and J. L. Cleveland (1999)
Genes & Dev. 13, 2658-2669
   Abstract »    Full Text »
Components of an SCF ubiquitin ligase localize to the centrosome and regulate the centrosome duplication cycle.
E. Freed, K. R. Lacey, P. Huie, S. A. Lyapina, R. J. Deshaies, T. Stearns, and P. K. Jackson (1999)
Genes & Dev. 13, 2242-2257
   Abstract »    Full Text »
How many tumor suppressor genes are involved in human lung carcinogenesis?.
T. Kohno and J. Yokota1 (1999)
Carcinogenesis 20, 1403-1410
   Abstract »    Full Text »    PDF »
Apoptotic and Mitotic Indices Predict Survival Rates in Lymph Node-negative Colon Carcinomas.
F. A. Sinicrope, J. Hart, H.-A. Hsu, M. Lemoine, F. Michelassi, and L. C. Stephens (1999)
Clin. Cancer Res. 5, 1793-1804
   Abstract »    Full Text »    PDF »
Budding Yeast Bub2 Is Localized at Spindle Pole Bodies and Activates the Mitotic Checkpoint via a Different Pathway from Mad2.
R. Fraschini, E. Formenti, G. Lucchini, and S. Piatti (1999)
J. Cell Biol. 145, 979-991
   Abstract »    Full Text »    PDF »
Centrosome Abnormalities in Pancreatic Ductal Carcinoma.
N. Sato, K. Mizumoto, M. Nakamura, K. Nakamura, M. Kusumoto, H. Niiyama, T. Ogawa, and M. Tanaka (1999)
Clin. Cancer Res. 5, 963-970
   Abstract »    Full Text »    PDF »
Roles of the functional loss of p53 and other genes inastrocytoma tumorigenesis and progression.
M. Nozaki, M. Tada, H. Kobayashi, C.-L. Zhang, Y. Sawamura, H. Abe, N. Ishii, and E. G. Van Meir (1999)
Neuro Oncology 1, 124-137
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)