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Science 16 February 1996:
Vol. 271. no. 5251, pp. 981 - 983
DOI: 10.1126/science.271.5251.981
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Reports

Role of Rho in Chemoattractant-Activated Leukocyte Adhesion Through Integrins

Carlo Laudanna (1),  James J. Campbell,  Eugene C. Butcher

Heterotrimeric guanine nucleotide binding protein (G protein)-linked receptors of the chemoattractant subfamily can trigger adhesion through leukocyte integrins, and in this role they are thought to regulate immune cell-cell interactions and trafficking. In lymphoid cells transfected with formyl peptide or interleukin-8 receptors, agonist stimulation activated nucleotide exchange on the small guanosine triphosphate-binding protein RhoA in seconds. Inactivation of Rho by C3 transferase exoenzyme blocked agonist-induced lymphocyte alpha4beta1 adhesion to vascular cell adhesion molecule-1 and neutrophil beta2 integrin adhesion to fibrinogen. These findings suggest that Rho participates in signaling from chemoattractant receptors to trigger rapid adhesion in leukocytes.


Laboratory of Immunology and Vascular Biology, Department of Pathology, and Digestive Disease Center, Stanford University, Stanford, CA 94305, USA, and Center for Molecular Biology and Medicine, Veterans Affairs Health Care System, Palo Alto, CA 94304, USA.
(1) To whom correspondence should be addressed.


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Blood 93, 3713-3722
   Abstract »    Full Text »    PDF »
R-Ras Signals through Specific Integrin {alpha} Cytoplasmic Domains to Promote Migration and Invasion of Breast Epithelial Cells.
P. J. Keely, E. V. Rusyn, A. D. Cox, and L. V. Parise (1999)
J. Cell Biol. 145, 1077-1088
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Rho and Rho Kinase Mediate Thrombin-Stimulated Vascular Smooth Muscle Cell DNA Synthesis and Migration.
T. M. Seasholtz, M. Majumdar, D. D. Kaplan, and J. H. Brown (1999)
Circ. Res. 84, 1186-1193
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Characterization of Rac and Cdc42 Activation in Chemoattractant-stimulated Human Neutrophils Using a Novel Assay for Active GTPases.
V. Benard, B. P. Bohl, and G. M. Bokoch (1999)
J. Biol. Chem. 274, 13198-13204
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Differential Regulation of {beta}1 Integrins by Chemoattractants Regulates Neutrophil Migration through Fibrin.
J. D. Loike, L. Cao, S. Budhu, E. E. Marcantonio, J. E. Khoury, S. Hoffman, T. A. Yednock, and S. C. Silverstein (1999)
J. Cell Biol. 144, 1047-1056
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Chemoattractant Receptor Cross-desensitization.
H. Ali, R. M. Richardson, B. Haribabu, and R. Snyderman (1999)
J. Biol. Chem. 274, 6027-6030
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Identification and characterization of ligands for L-selectin in the kidney. I. Versican, a large chondroitin sulfate proteoglycan, is a ligand for L-selectin.
H. Kawashima, Y.-F. Li, N. Watanabe, J. Hirose, M. Hirose, and M. Miyasaka (1999)
Int. Immunol. 11, 393-405
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Lymphocyte Migration Through Brain Endothelial Cell Monolayers Involves Signaling Through Endothelial ICAM-1 Via a Rho-Dependent Pathway.
P. Adamson, S. Etienne, P.-O. Couraud, V. Calder, and J. Greenwood (1999)
J. Immunol. 162, 2964-2973
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Matrix Valency Regulates Integrin-mediated Lymphoid Adhesion via Syk Kinase.
D. G. Stupack, E. Li, S. A. Silletti, J. A. Kehler, R. L. Geahlen, K. Hahn, G. R. Nemerow, and D. A. Cheresh (1999)
J. Cell Biol. 144, 777-788
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Impaired Antibacterial Host Defense in Mice Lacking the N-formylpeptide Receptor.
J.-L. Gao, E. J. Lee, and P. M. Murphy (1999)
J. Exp. Med. 189, 657-662
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Deamidation of Cdc42 and Rac by Escherichia coli Cytotoxic Necrotizing Factor 1: Activation of c-Jun N-Terminal Kinase in HeLa Cells.
M. Lerm, J. Selzer, A. Hoffmeyer, U. R. Rapp, K. Aktories, and G. Schmidt (1999)
Infect. Immun. 67, 496-503
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High Affinity Very Late Antigen-4 Subsets Expressed on T Cells Are Mandatory for Spontaneous Adhesion Strengthening But Not for Rolling on VCAM-1 in Shear Flow.
C. Chen, J. L. Mobley, O. Dwir, F. Shimron, V. Grabovsky, R. R. Lobb, Y. Shimizu, and R. Alon (1999)
J. Immunol. 162, 1084-1095
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Inhibition of Experimental Autoimmune Encephalomyelitis by a Tyrosine Kinase Inhibitor.
G. Constantin, C. Laudanna, S. Brocke, and E. C. Butcher (1999)
J. Immunol. 162, 1144-1149
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Cell adhesion and Rho small GTPases.
M Fukata, M Nakagawa, S Kuroda, and K Kaibuchi (1999)
J. Cell Sci. 112, 4491-4500
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Monocyte Adherence Induced by Lipopolysaccharide Involves CD14, LFA-1, and Cytohesin-1. REGULATION BY Rho AND PHOSPHATIDYLINOSITOL 3-KINASE.
Z. Hmama, K. L. Knutson, P. Herrera-Velit, D. Nandan, and N. E. Reiner (1999)
J. Biol. Chem. 274, 1050-1057
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MAP Kinase Pathways in the Yeast Saccharomyces cerevisiae.
M. C. Gustin, J. Albertyn, M. Alexander, and K. Davenport (1998)
Microbiol. Mol. Biol. Rev. 62, 1264-1300
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Sequence Motifs in the Integrin {alpha}4 Cytoplasmic Tail Required for Regulation of In Vivo Expansion of Murine Lymphoma Cells.
M. Bittner, U. Gosslar, A. Luz, and B. Holzmann (1998)
J. Immunol. 161, 5978-5986
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Evidence of zeta  Protein Kinase C Involvement in Polymorphonuclear Neutrophil Integrin-dependent Adhesion and Chemotaxis.
C. Laudanna, D. Mochly-Rosen, T. Liron, G. Constantin, and E. C. Butcher (1998)
J. Biol. Chem. 273, 30306-30315
   Abstract »    Full Text »    PDF »


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