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Science 2 February 1996:
Vol. 271. no. 5249, pp. 659 - 662
DOI: 10.1126/science.271.5249.659
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Reports

Molecular Detection of Primary Bladder Cancer by Microsatellite Analysis

Li Mao,  Mark P. Schoenberg,  Marshall Scicchitano,  Yener S. Erozan,  Adrian Merlo,  Donna Schwab,  David Sidransky (1)

Microsatellite DNA markers have been widely used as a tool for the detection of loss of heterozygosity and genomic instability in primary tumors. In a blinded study, urine samples from 25 patients with suspicious bladder lesions that had been identified cystoscopically were analyzed by this molecular method and by conventional cytology. Microsatellite changes matching those in the tumor were detected in the urine sediment of 19 of the 20 patients (95 percent) who were diagnosed with bladder cancer, whereas urine cytology detected cancer cells in 9 of 18 (50 percent) of the samples. These results suggest that microsatellite analysis, which in principle can be performed at about one-third the cost of cytology, may be a useful addition to current screening methods for detecting bladder cancer.


L. Mao, M. Scicchitano, A. Merlo, D. Schwab, D. Sidransky, Department of Otolaryngology-Head and Neck Surgery, Division of Head and Neck Cancer Research, Johns Hopkins University School of Medicine, 818 Ross Research, 720 Rutland Avenue, Baltimore, MD 21205-2196, USA.
M. P. Schoenberg, Department of Urology, Johns Hopkins Hospital, 600 North Wolfe Street, Baltimore, MD 21287, USA.
Y. S. Erozan, Department of Pathology, Johns Hopkins Hospital, 600 North Wolfe Street, Baltimore, MD 21287, USA.
(1) To whom correspondence should be addressed.


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