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Science 22 December 1995:
Vol. 270. no. 5244, pp. 2005 - 2008
DOI: 10.1126/science.270.5244.2005
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Reports

The white Gene of Ceratitis capitata: A Phenotypic Marker for Germline Transformation

Laurence J. Zwiebel,  Giuseppe Saccone,  Antigone Zacharopoulou,  Nora J. Besansky,  Guido Favia (1),  Frank H. Collins,  Christos Louis,  Fotis C. Kafatos (2)

Reliable germline transformation is required for molecular studies and ultimately for genetic control of economically important insects, such as the Mediterranean fruit fly (medfly) Ceratitis capitata. A prerequisite for the establishment and maintenance of transformant lines is selectable or phenotypically dominant markers. To this end, a complementary DNA clone derived from the medfly white gene was isolated, which showed substantial similarity to white genes in Drosophila melanogaster and other Diptera. It is correlated with a spontaneous mutation causing white eyes in the medfly and can be used to restore partial eye color in transgenic Drosophila carrying a null mutation in the endogenous white gene.


L. J . Zwiebel and F. C. Kafatos, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany; Institute of Molecular Biology and Biotechnology-FORTH, Heraklion, Crete, Greece; and Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA, 02138, USA.
G. Saccone, Dipartimento di Genetica, Biologia Generale e Molecolare, Università Federico II di Napoli, Napoli, Italy.
A. Zacharopoulou, Department of Biology, University of Patras, Patras, Greece.
N. J. Besansky and F. H. Collins, Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA.
G. Favia and C. Louis, Institute of Molecular Biology and Biotechnology-FORTH, Heraklion, Crete, Greece.
(1) Present address: Istituto di Parassitologia, Universita di Roma I-00185 Roma, Italy.
(2) To whom correspondence should be addressed at EMBL, Meyerhofstrasse 1, D-69117, Heidelberg, Germany.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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T. G. Loukeris, I. Livadaras, B. Arcą, S. Zabalou, and C. Savakis (1995)
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