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Science 8 December 1995:
Vol. 270. no. 5242, pp. 1681 - 1683
DOI: 10.1126/science.270.5242.1681
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Reports

Pineal Serotonin N-Acetyltransferase: Expression Cloning and Molecular Analysis

Steven L. Coon,  Patrick H. Roseboom,  Ruben Baler,  Joan L. Weller,  M. A. A. Namboodiri,  Eugene V. Koonin,  David C. Klein (1)

Pineal serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, or AA-NAT) generates the large circadian rhythm in melatonin, the hormone that coordinates daily and seasonal physiology in some mammals. Complementary DNA encoding ovine AA-NAT was cloned. The abundance of AA-NAT messenger RNA (mRNA) during the day was high in the ovine pineal gland and somewhat lower in retina. AA-NAT mRNA was found unexpectedly in the pituitary gland and in some brain regions. The night-to-day ratio of ovine pineal AA-NAT mRNA is less than 2. In contrast, the ratio exceeds 150 in rats. AA-NAT represents a family within a large superfamily of acetyltransferases.


S. L. Coon, P. H. Roseboom, R. Baler, J. L. Weller, D. C. Klein, Section on Neuroendocrinology, Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
M. A. A. Namboodiri, Department of Biology, Georgetown University, Washington, D. C. 20057, USA.
E. V. Koonin, National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA.
(1) To whom correspondence should be addressed.


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p300/CBP-associated Factor Histone Acetyltransferase Processing of a Peptide Substrate. KINETIC ANALYSIS OF THE CATALYTIC MECHANISM.
O. D. Lau, A. D. Courtney, A. Vassilev, L. A. Marzilli, R. J. Cotter, Y. Nakatani, and P. A. Cole (2000)
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cAMP Regulation of Arylalkylamine N-Acetyltransferase (AANAT, EC 2.3.1.87). A NEW CELL LINE (1E7) PROVIDES EVIDENCE OF INTRACELLULAR AANAT ACTIVATION.
S. L. Coon, J. L. Weller, H.-W. Korf, M. A. A. Namboodiri, M. Rollag, and D. C. Klein (2001)
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Induction of photosensitivity in neonatal rat pineal gland.
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PNAS 97, 11540-11544
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