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Science 8 December 1995:
Vol. 270. no. 5242, pp. 1671 - 1674
DOI: 10.1126/science.270.5242.1671
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Reports

Separation of Origin Recognition Complex Functions by Cross-Species Complementation

Ann E. Ehrenhofer-Murray,  Manfred Gossen,  Daniel T. S. Pak,  Michael R. Botchan,  Jasper Rine (1)

Transcriptional silencing at the HMRa locus of Saccharomyces cerevisiae requires the function of the origin recognition complex (ORC), the replication initiator of yeast. Expression of a Drosophila melanogaster Orc2 complementary DNA in the yeast orc2-1 strain, which is defective for replication and silencing, complemented the silencing defect but not the replication defect; this result indicated that the replication and silencing functions of ORC were separable. The orc2-1 mutation mapped to the region of greatest homology between the Drosophila and yeast proteins. The silent state mediated by DmOrc2 was epigenetic; it was propagated during mitotic divisions in a relatively stable way, whereas the nonsilent state was metastable. In contrast, the silent state was erased during meiosis.



A. E. Ehrenhofer-Murray and J. Rine, Division of Genetics, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
M. Gossen, D. T. S. Pak, M. R. Botchan, Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, 401 Barker Hall, University of California, Berkeley, CA 94720, USA.
(1) To whom correspondence should be addressed. E-mail: jasper{at}mendel.berkeley.edu


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