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Science 17 November 1995:
Vol. 270. no. 5239, pp. 1213 - 1215
DOI: 10.1126/science.270.5239.1213
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Reports

Requirement of Saccharomyces cerevisiae Ras for Completion of Mitosis

Takashi Morishita,  Hiroshi Mitsuzawa,  Masato Nakafuku,  Shun Nakamura,  Seisuke Hattori (1),  Yasuhiro Anraku

In the yeast Saccharomyces cerevisiae, Ras regulates adenylate cyclase, which is essential for progression through the G(1) phase of the cell cycle. However, even when the adenosine 3`,5`-monophosphate (cAMP) pathway was bypassed, the double disruption of RAS1 and RAS2 resulted in defects in growth at both low and high temperatures. Furthermore, the simultaneous disruption of RAS1, RAS2, and the RAS-related gene RSR1 was lethal at any temperature. The triple-disrupted cells were arrested late in the mitotic (M) phase, which was accompanied by an accumulation of cells with divided chromosomes and sustained histone H1 kinase activity. The lethality of the triple disruption was suppressed by the multicopies of CDC5, CDC15, DBF2, SPO12, and TEM1, all of which function in the completion of the M phase. Mammalian ras also suppressed the lethality, which suggests that a similar signaling pathway exists in higher eukaryotes. These results demonstrate that S. cerevisiae Ras functions in the completion of the M phase in a manner independent of the Ras-cAMP pathway.


T. Morishita, Division of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, Japan, and Department of Plant Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan.
H. Mitsuzawa, Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan.
M. Nakafuku, S. Nakamura, S. Hattori, Division of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, Japan.
Y. Anraku, Department of Plant Sciences, Graduate School of Science, University of Tokyo, Tokyo 113, Japan.
(1) To whom correspondence should be addressed.


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A Role for the Noncatalytic N Terminus in the Function of Cdc25, a Saccharomyces cerevisiae Ras-Guanine Nucleotide Exchange Factor.
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Characterization of Raf-1 Activation in Mitosis.
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Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae.
M. D. Mendenhall and A. E. Hodge (1998)
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Sequential Assembly of Myosin II, an IQGAP-like Protein, and Filamentous Actin to a Ring Structure Involved in Budding Yeast Cytokinesis.
J. Lippincott and R. Li (1998)
J. Cell Biol. 140, 355-366
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MOB1, an Essential Yeast Gene Required for Completion of Mitosis and Maintenance of Ploidy.
F. C. Luca and M. Winey (1998)
Mol. Biol. Cell 9, 29-46
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Ras2 and Ras1 Protein Phosphorylation in Saccharomyces cerevisiae.
J. L. Whistler and J. Rine (1997)
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Human Bcl-2 Reverses Survival Defects in Yeast Lacking Superoxide Dismutase and Delays Death of Wild-Type Yeast.
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J. Cell Biol. 137, 1581-1588
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The PPS1 Gene of Saccharomyces cerevisiae Codes for a Dual Specificity Protein Phosphatase with a Role in the DNA Synthesis Phase of the Cell Cycle.
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