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Science 10 November 1995:
Vol. 270. no. 5238, pp. 994 - 997
DOI: 10.1126/science.270.5238.994
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Reports

Transition in Specification of Embryonic Metazoan DNA Replication Origins

Olivier Hyrien (1) (2),  Chrystelle Maric (2),  Marcel Méchali (1)

In early Xenopus embryos, in which ribosomal RNA genes (rDNA) are not transcribed, rDNA replication initiates and terminates at 9- to 12-kilobase pair intervals, with no detectable dependence on specific DNA sequences. Resumption of ribosomal RNA (rRNA) synthesis at late blastula and early gastrula is accompanied by a specific repression of replication initiation within transcription units; the frequency of initiation within intergenic spacers remains as high as in early blastula. These results demonstrate that for rRNA genes, circumscribed zones of replication initiation emerge in intergenic DNA during the time in metazoan development when the chromatin is remodeled to allow gene transcription.


Molecular Embryology, Institut Jacques Monod, 75 251 Paris Cedex 05, France.
(1) To whom correspondence should be addressed.
(2) Present address: Génétique Moléculaire, Département de Biologie, Ecole Normale Supérieure, 46 rue d'Ulm, 75230 Paris Cedex 05, France.


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J. Walter and J. W. Newport (1997)
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Functional compartmentalization of the nucleus.
J Strouboulis and A. Wolffe (1996)
J. Cell Sci. 109, 1991-2000
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Aphidicolin Triggers a Block to Replication Origin Firing in Xenopus Egg Extracts.
K. Marheineke and O. Hyrien (2001)
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