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Science 10 November 1995:
Vol. 270. no. 5238, pp. 935 - 941
DOI: 10.1126/science.270.5238.935
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Articles

Protein Design: A Hierarchic Approach

James W. Bryson,  Stephen F. Betz,  Helen S. Lu,  Daniel J. Suich,  Hongxing X. Zhou,  Karyn T. O'Neil,  William F. DeGrado (1)

The de novo design of peptides and proteins has recently emerged as an approach for investigating protein structure and function. Designed, helical peptides provide model systems for dissecting and quantifying the multiple interactions that stabilize secondary structure formation. De novo design is also useful for exploring the features that specify the stoichiometry and stability of alpha-helical coiled coils and for defining the requirements for folding into structures that resemble native, functional proteins. The design process often occurs in a series of discrete steps. Such steps reflect the hierarchy of forces required for stabilizing tertiary structures, beginning with hydrophobic forces and adding more specific interactions as required to achieve a unique, functional protein.


The authors are at DuPont Merck Pharmaceutical Company, P.O. Box 80328, Wilmington, DE 19880, USA.
(1) To whom correspondence should be addressed. E-mail: degradwf{at}lldmpc.dnet.dupont.com


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