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Science 3 November 1995:
Vol. 270. no. 5237, pp. 789 - 791
DOI: 10.1126/science.270.5237.789
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Reports

Aberrant Subcellular Localization of BRCA1 in Breast Cancer

Yumay Chen,  Chi-Fen Chen,  Daniel J. Riley,  D. Craig Allred,  Phang-Lang Chen,  Daniel Von Hoff,  C. Kent Osborne,  Wen-Hwa Lee (1)

The BRCA1 gene product was identified as a 220-kilodalton nuclear phosphoprotein in normal cells, including breast ductal epithelial cells, and in 18 of 20 tumor cell lines derived from tissues other than breast and ovary. In 16 of 17 breast and ovarian cancer lines and 17 of 17 samples of cells obtained from malignant effusions, however, BRCA1 localized mainly in cytoplasm. Absence of BRCA1 or aberrant subcellular location was also observed to a variable extent in histological sections of many breast cancer biopsies. These findings suggest that BRCA1 abnormalities may be involved in the pathogenesis of many breast cancers, sporadic as well as familial.


Y. Chen, C.-F. Chen, D. J. Riley, P.-L. Chen, W.-H. Lee, Center for Molecular Medicine/Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78245, USA.
D. C. Allred, Department of Pathology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78284, USA.
D. Von Hoff and C. K. Osborne, Division of Medical Oncology, Department of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX 78284, USA.
(1) To whom correspondence should be addressed.


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