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Science 20 October 1995:
Vol. 270. no. 5235, pp. 467 - 470
DOI: 10.1126/science.270.5235.467
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Reports

Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray

Mark Schena (1),  Dari Shalon (1),  Ronald W. Davis (2),  Patrick O. Brown (3)

A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential expression measurements of 45 Arabidopsis genes were made by means of simultaneous, two-color fluorescence hybridization.


M. Schena and R. W. Davis, Department of Biochemistry, Beckman Center, Stanford University Medical Center, Stanford, CA 94305, USA.
D. Shalon and P. O. Brown, Department of Biochemistry and Howard Hughes Medical Institute, Beckman Center, Stanford University Medical Center, Stanford, CA 94305, USA.
(1) These authors contributed equally to this work.
(2) Present address: Synteni, Palo Alto, CA 94303, USA.
(3) To whom correspondence should be addressed. E-mail: pbrown{at}cmgm.stanford.edu


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