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Science 6 October 1995:
Vol. 270. no. 5233, pp. 86 - 90
DOI: 10.1126/science.270.5233.86
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Reports

Myt1: A Membrane-Associated Inhibitory Kinase That Phosphorylates Cdc2 on Both Threonine-14 and Tyrosine-15

Paul R. Mueller,  Thomas R. Coleman,  Akiko Kumagai,  William G. Dunphy (1)

Cdc2 is the cyclin-dependent kinase that controls entry of cells into mitosis. Phospho-rylation of Cdc2 on threonine-14 and tyrosine-15 inhibits the activity of the enzyme and prevents premature initiation of mitosis. Although Wee1 has been identified as the kinase that phosphorylates tyrosine-15 in various organisms, the threonine-14-specific kinase has not been isolated. A complementary DNA was cloned from Xenopus that encodes Myt1, a member of the Wee1 family that was discovered to phosphorylate Cdc2 efficiently on both threonine-14 and tyrosine-15. Myt1 is a membrane-associated protein that contains a putative transmembrane segment. Immunodepletion studies suggested that Myt1 is the predominant threonine-14-specific kinase in Xenopus egg extracts. Myt1 activity is highly regulated during the cell cycle, suggesting that this relative of Wee1 plays a role in mitotic control.


Division of Biology 216-76, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA.
(1) To whom correspondence should be addressed.


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   Abstract »    Full Text »    PDF »
Xe-p9, a Xenopus Suc1/Cks protein, is essential for the Cdc2-dependent phosphorylation of the anaphase- promoting complex at mitosis.
D. Patra and W. G. Dunphy (1998)
Genes & Dev. 12, 2549-2559
   Abstract »    Full Text »
Control of Cyclin B1 localization through regulated binding of the nuclear export factor CRM1.
J. Yang, E. S.G. Bardes, J. D. Moore, J. Brennan, M. A. Powers, and S. Kornbluth (1998)
Genes & Dev. 12, 2131-2143
   Abstract »    Full Text »
Activated Polo-Like Kinase Plx1 Is Required at Multiple Points during Mitosis in Xenopus laevis.
Y.-W. Qian, E. Erikson, C. Li, and J. L. Maller (1998)
Mol. Cell. Biol. 18, 4262-4271
   Abstract »    Full Text »
The essential mitotic peptidyl-prolyl isomerase Pin1 binds and regulates mitosis-specific phosphoproteins.
M. Shen, P. T. Stukenberg, M. W. Kirschner, and K. P. Lu (1998)
Genes & Dev. 12, 706-720
   Abstract »    Full Text »
14-3-3 Proteins Act as Negative Regulators of the Mitotic Inducer Cdc25 in Xenopus Egg Extracts.
A. Kumagai, P. S. Yakowec, and W. G. Dunphy (1998)
Mol. Biol. Cell 9, 345-354
   Abstract »    Full Text »
The Drosophila POLO kinase localises to multiple compartments of the mitotic apparatus and is required for the phosphorylation of MPM2 reactive epitopes.
E Logarinho and C. Sunkel (1998)
J. Cell Sci. 111, 2897-2909
   Abstract »    PDF »
The cdc25B phosphatase is essential for the G2/M phase transition in human cells.
C Lammer, S Wagerer, R Saffrich, D Mertens, W Ansorge, and I Hoffmann (1998)
J. Cell Sci. 111, 2445-2453
   Abstract »    PDF »
The Polo-like kinase Plx1 is a component of the MPF amplification loop at the G2/M-phase transition of the cell cycle in Xenopus eggs.
A Abrieu, T Brassac, S Galas, D Fisher, J. Labbe, and M Doree (1998)
J. Cell Sci. 111, 1751-1757
   Abstract »    PDF »
Analysis of the early embryonic cell cycles of Xenopus; regulation of cell cycle length by Xe-wee1 and Mos.
M. Murakami and G. Vande Woude (1998)
Development 125, 237-248
   Abstract »    PDF »
Cell Cycle-regulated Expression, Phosphorylation, and Degradation of p55Cdc. A MAMMALIAN HOMOLOG OF CDC20/Fizzy/slp1.
J. Weinstein (1997)
J. Biol. Chem. 272, 28501-28511
   Abstract »    Full Text »    PDF »
Induction of a G2-Phase Arrest in Xenopus Egg Extracts by Activation of p42 Mitogen-activated Protein Kinase.
S. A. Walter, T. M. Guadagno, and J. E. Ferrell Jr. (1997)
Mol. Biol. Cell 8, 2157-2169
   Abstract »    Full Text »
A Dual-Specificity Phosphatase Cdc25B Is an Unstable Protein and Triggers p34cdc2/Cyclin B Activation in Hamster BHK21 Cells Arrested with Hydroxyurea.
H. Nishijima, H. Nishitani, T. Seki, and T. Nishimoto (1997)
J. Cell Biol. 138, 1105-1116
   Abstract »    Full Text »    PDF »
Human Myt1 Is a Cell Cycle-regulated Kinase That Inhibits Cdc2 but Not Cdk2 Activity.
R. N. Booher, P. S. Holman, and A. Fattaey (1997)
J. Biol. Chem. 272, 22300-22306
   Abstract »    Full Text »    PDF »


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