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Science 6 October 1995:
Vol. 270. no. 5233, pp. 105 - 108
DOI: 10.1126/science.270.5233.105
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Reports

Dependence of Peptide Binding by MHC Class I Molecules on Their Interaction with TAP

Andres G. Grandea III,  Matthew J. Androlewicz,  Raghbir S. Athwal,  Daniel E. Geraghty,  Thomas Spies (1)

Major histocompatibility complex (MHC) class I molecules bind peptides that are delivered from the cytosol into the endoplasmic reticulum by the MHC-encoded transporter associated with antigen processing (TAP). Peptide capture by immature heterodimers of class I heavy chains and beta(2)-microglobulin may be facilitated by their physical association with TAP. A genetic defect in a human mutant cell line causes the complete failure of diverse class I heterodimers to associate with TAP. This deficiency impairs the ability of the class I heterodimers to efficiently capture peptides and results from loss of function of an unidentified gene or genes linked to the MHC.


A. G. Grandea III, D. E. Geraghty, T. Spies, Clinical Research Division, Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, WA 98104, USA.
M. J. Androlewicz, Immunology Program, H. Lee Moffitt Cancer Center and Research Institute and Department of Biochemistry and Molecular Biology, University of South Florida College of Medicine, Tampa, FL 33612, USA.
R. S. Athwal, Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, 3420 North Broad Street, Philadelphia, PA 19104, USA.
(1) To whom correspondence should be addressed. E-mail: tspies{at}fred.fhcrc.org


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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »


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