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Science 29 September 1995:
Vol. 269. no. 5232, pp. 1883 - 1885
DOI: 10.1126/science.7569932
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Articles

Science, Vol 269, Issue 5232, 1883-1885
Copyright © 1995 by American Association for the Advancement of Science

articles

FFA-1, a protein that promotes the formation of replication centers within nuclei

H Yan and J Newport

University of California, San Diego, Department of Biology, La Jolla, CA 92093, USA.

In the nuclei of eukaryotic cells, initiation of DNA replication occurs at a discrete number of foci. One component of these foci is the DNA replication factor RP-A. Here, the process leading to the association of RP-A with foci was reconstituted with cytosolic fractions derived from Xenopus eggs. With the use of this fractionated system, a 170-kilodalton protein required for the assembly of RP-A into foci was identified and purified. The protein appears to be an integral component of the foci at which replication of DNA is initiated in eukaryotic nuclei.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identification of the Xenopus DNA2 protein as a major nuclease for the 5'->3' strand-specific processing of DNA ends.
S. Liao, T. Toczylowski, and H. Yan (2008)
Nucleic Acids Res. 36, 6091-6100
   Abstract »    Full Text »    PDF »
Mechanistic Analysis of a DNA End Processing Pathway Mediated by the Xenopus Werner Syndrome Protein.
T. Toczylowski and H. Yan (2006)
J. Biol. Chem. 281, 33198-33205
   Abstract »    Full Text »    PDF »
Accumulation of FFA-1, the Xenopus Homolog of Werner Helicase, and DNA Polymerase {delta} on Chromatin in Response to Replication Fork Arrest.
N. Sasakawa, T. Fukui, and S. Waga (2006)
J. Biochem. 140, 95-103
   Abstract »    Full Text »    PDF »
RPA is an initiation factor for human chromosomal DNA replication.
D. Szuts, L. Kitching, C. Christov, A. Budd, S. Peak-Chew, and T. Krude (2003)
Nucleic Acids Res. 31, 1725-1734
   Abstract »    Full Text »    PDF »
Focus-formation of replication protein A, activation of checkpoint system and DNA repair synthesis induced by DNA double-strand breaks in Xenopus egg extract.
T. Kobayashi, S. Tada, T. Tsuyama, H. Murofushi, M. Seki, and T. Enomoto (2002)
J. Cell Sci. 115, 3159-3169
   Abstract »    Full Text »    PDF »
Evidence for a Replication Function of FFA-1, the Xenopus Orthologue of Werner Syndrome Protein.
C.-Y. Chen, J. Graham, and H. Yan (2001)
J. Cell Biol. 152, 985-996
   Abstract »    Full Text »    PDF »
Disruption of Nuclear Lamin Organization Blocks the Elongation Phase of DNA Replication.
R. D. Moir, T. P. Spann, H. Herrmann, and R. D. Goldman (2000)
J. Cell Biol. 149, 1179-1192
   Abstract »    Full Text »    PDF »
The yeast Sgs1p helicase acts upstream of Rad53p in the DNA replication checkpoint and colocalizes with Rad53p in S-phase-specific foci.
C. Frei and S. M. Gasser (2000)
Genes & Dev. 14, 81-96
   Abstract »    Full Text »
Mcm2, but Not RPA, Is a Component of the Mammalian Early G1-Phase Prereplication Complex.
D. S. Dimitrova, I. T. Todorov, T. Melendy, and D. M. Gilbert (1999)
J. Cell Biol. 146, 709-722
   Abstract »    Full Text »    PDF »
Functional and Physical Interaction between WRN Helicase and Human Replication Protein A.
R. M. Brosh Jr., D. K. Orren, J. O. Nehlin, P. H. Ravn, M. K. Kenny, A. Machwe, and V. A. Bohr (1999)
J. Biol. Chem. 274, 18341-18350
   Abstract »    Full Text »    PDF »
Nucleoskeleton and initiation of DNA replication in metazoan cells.
J. Ortega and M. DePamphilis (1999)
J. Cell Sci. 111, 3663-3673
   Abstract »    PDF »
Chromatin Association of Replication Protein A.
K. Treuner, C. Eckerich, and R. Knippers (1998)
J. Biol. Chem. 273, 31744-31750
   Abstract »    Full Text »    PDF »
Histone H1 Reduces the Frequency of Initiation in Xenopus Egg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin.
Z. H. Lu, D. B. Sittman, P. Romanowski, and G. H. Leno (1998)
Mol. Biol. Cell 9, 1163-1176
   Abstract »    Full Text »
Disruption of Nuclear Lamin Organization Alters the Distribution of Replication Factors and Inhibits DNA Synthesis.
T. P. Spann, R. D. Moir, A. E. Goldman, R. Stick, and R. D. Goldman (1997)
J. Cell Biol. 136, 1201-1212
   Abstract »    Full Text »    PDF »


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