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Science 29 September 1995:
Vol. 269. no. 5232, pp. 1869 - 1872
DOI: 10.1126/science.7569927
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Articles

Science, Vol 269, Issue 5232, 1869-1872
Copyright © 1995 by American Association for the Advancement of Science

articles

A specialized pathway affecting virulence glycoconjugates of Leishmania

A Descoteaux, Y Luo, SJ Turco, and SM Beverley

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.

For virulence and transmission, the protozoan parasite Leishmania must assemble a complex glycolipid on the cell surface, the lipophosphoglycan (LPG). Functional complementation identified the gene LPG2, which encodes an integral Golgi membrane protein implicated in intracellular compartmentalization of LPG biosynthesis. Ipg2- mutants lack only characteristic disaccharide-phosphate repeats, normally present on both LPG and other surface or secreted molecules considered critical for infectivity. In contrast, a related yeast gene, VAN2/VRG4, is essential and required for general Golgi function. These results suggest that LPG2 participates in a specialized virulence pathway, which may offer an attractive target for chemotherapy.


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