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Science 22 September 1995:
Vol. 269. no. 5231, pp. 1723 - 1726
DOI: 10.1126/science.7569901
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Articles

Science, Vol 269, Issue 5231, 1723-1726
Copyright © 1995 by American Association for the Advancement of Science

articles

Imaging elementary events of calcium release in skeletal muscle cells

A Tsugorka, E Rios, and LA Blatter

Department of Molecular Biophysics and Physiology, Rush University, Chicago, IL 60612, USA.

In skeletal muscle cells, calcium release to trigger contraction occurs at triads, specialized junctions where sarcoplasmic reticulum channels are opened by voltage sensors in the transverse tubule. Scanning confocal microscopy was used in cells under voltage clamp to measure the concentration of intracellular calcium, [Ca2+]i, at individual triads and [Ca2+]i gradients that were proportional to calcium release. In cells stimulated with small depolarizations, the [Ca2+]i gradients broke down into elementary events, corresponding to single-channel currents of about 0.1 picoampere. Because these events were one-tenth to one-fifth the size of calcium sparks (elementary release events of cardiac muscle), skeletal muscle control mechanisms appear to be fundamentally different.


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M. T Kirber, E. F Etter, K. A Bellve, L. M Lifshitz, R. A Tuft, F. S Fay, J. V Walsh Jr, and K. E Fogarty (2001)
J. Physiol. 531, 315-327
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Origin Sites of Calcium Release and Calcium Oscillations in Frog Sympathetic Neurons.
S. I. McDonough, Z. Cseresnyes, and M. F. Schneider (2000)
J. Neurosci. 20, 9059-9070
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Fast imaging in two dimensions resolves extensive sources of Ca2+ sparks in frog skeletal muscle.
G. Brum, A. Gonzalez, J. Rengifo, N. Shirokova, and E. Rios (2000)
J. Physiol. 528, 419-433
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Differential regulation of Ca2+ sparks and Ca2+ waves by UTP in rat cerebral artery smooth muscle cells.
J. H. Jaggar and M. T. Nelson (2000)
Am J Physiol Cell Physiol 279, C1528-C1539
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Two mechanisms for termination of individual Ca2+ sparks in skeletal muscle.
A. Lacampagne, M. G. Klein, C. W. Ward, and M. F. Schneider (2000)
PNAS 97, 7823-7828
   Abstract »    Full Text »    PDF »
Expression of ryanodine receptor RyR3 produces Ca2+ sparks in dyspedic myotubes.
C. W Ward, M. F Schneider, D. Castillo, F. Protasi, Y. Wang, S R Wayne Chen, and P. D Allen (2000)
J. Physiol. 525, 91-103
   Abstract »    Full Text »    PDF »
Measurement and Interpretation of Cytoplasmic [Ca2+] Signals From Calcium-Indicator Dyes.
S. M. Baylor and S. Hollingworth (2000)
Physiology 15, 19-26
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Calcium sparks in smooth muscle.
J. H. Jaggar, V. A. Porter, W. J. Lederer, and M. T. Nelson (2000)
Am J Physiol Cell Physiol 278, C235-C256
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Neuronal calcium sparks and intracellular calcium "noise".
N. Melamed-Book, S. G. Kachalsky, I. Kaiserman, and R. Rahamimoff (1999)
PNAS 96, 15217-15221
   Abstract »    Full Text »    PDF »
Spatially segregated control of Ca2+ release in developing skeletal muscle of mice.
N. Shirokova, R. Shirokov, D. Rossi, A. Gonzalez, W. G Kirsch, J. Garcia, V. Sorrentino, and E. Rios (1999)
J. Physiol. 521, 483-495
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Spatial and temporal aspects of calcium sparks in porcine tracheal smooth muscle cells.
C. M. Pabelick, Y. S. Prakash, M. S. Kannan, and G. C. Sieck (1999)
Am J Physiol Lung Cell Mol Physiol 277, L1018-L1025
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Ca channels in cardiac myocytes: structure and function in Ca influx and intracellular Ca release.
D. M Bers and E. Perez-Reyes (1999)
Cardiovasc Res 42, 339-360
   Abstract »    Full Text »    PDF »
A repetitive mode of activation of discrete Ca2+ release events (Ca2+ sparks) in frog skeletal muscle fibres.
M. G Klein, A. Lacampagne, and M. F Schneider (1999)
J. Physiol. 515, 391-411
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Ca2+ sparks activate K+ and Cl- channels, resulting in spontaneous transient currents in guinea-pig tracheal myocytes.
R. ZhuGe, S. M Sims, R. A Tuft, K. E Fogarty, and J. V Walsh Jr (1998)
J. Physiol. 513, 711-718
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Bay K 8644 Increases Resting Ca2+ Spark Frequency in Ferret Ventricular Myocytes Independent of Ca Influx : Contrast With Caffeine and Ryanodine Effects.
H. Satoh, H. Katoh, P. Velez, M. Fill, and D. M. Bers (1998)
Circ. Res. 83, 1192-1204
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Ontogeny of Local Sarcoplasmic Reticulum Ca2+ Signals in Cerebral Arteries : Ca2+ Sparks as Elementary Physiological Events.
M. Gollasch, G. C. Wellman, H. J. Knot, J. H. Jaggar, D. H. Damon, A. D. Bonev, and M. T. Nelson (1998)
Circ. Res. 83, 1104-1114
   Abstract »    Full Text »    PDF »
Calcium Release and Calcium-Activated Chloride Channels in Airway Smooth Muscle Cells.
M. I. KOTLIKOFF and Y.-X. WANG (1998)
Am. J. Respir. Crit. Care Med. 158, S109-S114
   Abstract »    Full Text »    PDF »
Local calcium release in mammalian skeletal muscle.
N. Shirokova, J. Garcia, and E. Rios (1998)
J. Physiol. 512, 377-384
   Abstract »    Full Text »    PDF »
Sorcin Associates with the Pore-forming Subunit of Voltage-dependent L-type Ca2+ Channels.
M. B. Meyers, T. S. Puri, A. J. Chien, T. Gao, P.-H. Hsu, M. M. Hosey, and G. I. Fishman (1998)
J. Biol. Chem. 273, 18930-18935
   Abstract »    Full Text »    PDF »
Voltage change-induced gating transitions of the rabbit skeletal muscle Ca2+ release channel.
A Zahradnikova and L G Meszaros (1998)
J. Physiol. 509, 29-38
   Abstract »    Full Text »    PDF »
A continuum of InsP3-mediated elementary Ca2+ signalling events in Xenopus oocytes.
X.-P. Sun, N. Callamaras, J. S Marchant, and I. Parker (1998)
J. Physiol. 509, 67-80
   Abstract »    Full Text »    PDF »
Variability in spontaneous subcellular calcium release in guinea-pig ileum smooth muscle cells.
D V Gordienko, T B Bolton, and M B Cannell (1998)
J. Physiol. 507, 707-720
   Abstract »    Full Text »    PDF »
Ca2+ Flux Through Promiscuous Cardiac Na+ Channels: Slip-Mode Conductance.
L. F. Santana, A. M. Gómez, and W. J. Lederer (1998)
Science 279, 1027-1033
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Effect of a 14-day hindlimb suspension on cytosolic Ca2+ concentration in rat portal vein myocytes.
J.-L. Morel, F.-X. Boittin, G. Halet, S. Arnaudeau, C. Mironneau, and J. Mironneau (1997)
Am J Physiol Heart Circ Physiol 273, H2867-H2875
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Elementary events of agonist-induced Ca2+ release in vascular endothelial cells.
J. Huser and L. A. Blatter (1997)
Am J Physiol Cell Physiol 273, C1775-C1782
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Voltage dependence of the pattern and frequency of discrete Ca2+ release events after brief repriming in frog skeletal muscle.
M. G. Klein, A. Lacampagne, and M. F. Schneider (1997)
PNAS 94, 11061-11066
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Adenovirus E1A Inhibits Cardiac Myocyte-specific Gene Expression through Its Amino Terminus.
N. H. Bishopric, G.-Q. Zeng, B. Sato, and K. A. Webster (1997)
J. Biol. Chem. 272, 20584-20594
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Sarcoplasmic reticulum Ca2+ release flux underlying Ca2+ sparks in cardiac muscle.
L. A. Blatter, J. Huser, and E. Rios (1997)
PNAS 94, 4176-4181
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Effects of FK-506 on Contraction and Ca2+ Transients in Rat Cardiac Myocytes.
E. McCall, L. Li, H. Satoh, T. R. Shannon, L. A. Blatter, and D. M. Bers (1996)
Circ. Res. 79, 1110-1121
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Selectively Suppressed Ca2+-induced Ca2+ Release Activity of alpha -Ryanodine Receptor (alpha -RyR) in Frog Skeletal Muscle Sarcoplasmic Reticulum. POTENTIAL DISTINCT MODES IN Ca2+ RELEASE BETWEEN alpha - AND beta -RyR.
T. Murayama and Y. Ogawa (2001)
J. Biol. Chem. 276, 2953-2960
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Contribution of Ryanodine Receptor Subtype 3 to Ca2+ Responses in Ca2+-overloaded Cultured Rat Portal Vein Myocytes.
J. Mironneau, F. Coussin, L. H. Jeyakumar, S. Fleischer, C. Mironneau, and N. Macrez (2001)
J. Biol. Chem. 276, 11257-11264
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Involvement of multiple intracellular release channels in calcium sparks of skeletal muscle.
A. Gonzalez, W. G. Kirsch, N. Shirokova, G. Pizarro, G. Brum, I. N. Pessah, M. D. Stern, H. Cheng, and E. Rios (2000)
PNAS 97, 4380-4385
   Abstract »    Full Text »    PDF »


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