Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 22 September 1995:
Vol. 269. no. 5231, pp. 1718 - 1721
DOI: 10.1126/science.7569899
Prev | Table of Contents | Next

Articles

Science, Vol 269, Issue 5231, 1718-1721
Copyright © 1995 by American Association for the Advancement of Science

articles

Activation of a G protein complex by aggregation of beta-1,4-galactosyltransferase on the surface of sperm

X Gong, DH Dubois, DJ Miller, and BD Shur

Department of Biochemistry and Molecular Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.

Fertilization is initiated by the species-specific binding of sperm to the extracellular coat of the egg. One sperm receptor for the mouse egg is beta-1,4-galactosyltransferase (GalTase), which binds O-linked oligosaccharides on the egg coat glycoprotein ZP3. ZP3 binding induces acrosomal exocytosis through the activation of a pertussis toxin-sensitive heterotrimeric guanine nucleotide-binding protein (G protein). The cytoplasmic domain of sperm surface GalTase bound to and activated a heterotrimeric G protein complex that contained the Gi alpha subunit. Aggregation of GalTase by multivalent ligands elicited G protein activation. Sperm from transgenic mice that overexpressed GalTase had higher rates of G protein activation than did wild-type sperm, which rendered transgenic sperm hypersensitive to their ZP3 ligand. Thus, the cytoplasmic domain of cell surface GalTase appears to enable it to function as a signal-transducing receptor for extracellular oligosaccharide ligands.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Expression of sialyltransferase activity on intact human neutrophils.
S. Rifat, T. J. Kang, D. Mann, L. Zhang, A. C. Puche, N. M. Stamatos, S. E. Goldblum, R. Brossmer, and A. S. Cross (2008)
J. Leukoc. Biol. 84, 1075-1081
   Abstract »    Full Text »    PDF »
Reduced fertility of mouse epididymal sperm lacking Prss21/Tesp5 is rescued by sperm exposure to uterine microenvironment.
M. Yamashita, A. Honda, A. Ogura, S.-i. Kashiwabara, K. Fukami, and T. Baba (2008)
Genes Cells 13, 1001-1013
   Abstract »    Full Text »    PDF »
Exploiting common targets in human fertilization and HIV infection: development of novel contraceptive microbicides.
G. F. Doncel (2006)
Hum. Reprod. Update 12, 103-117
   Abstract »    Full Text »    PDF »
Elevated {beta}1,4-Galactosyltransferase I in Highly Metastatic Human Lung Cancer Cells: IDENTIFICATION OF E1AF AS IMPORTANT TRANSCRIPTION ACTIVATOR.
X. Zhu, J. Jiang, H. Shen, H. Wang, H. Zong, Z. Li, Y. Yang, Z. Niu, W. Liu, X. Chen, et al. (2005)
J. Biol. Chem. 280, 12503-12516
   Abstract »    Full Text »    PDF »
Single Transmembrane Spanning Heterotrimeric G Protein-Coupled Receptors and Their Signaling Cascades.
T. B. Patel (2004)
Pharmacol. Rev. 56, 371-385
   Abstract »    Full Text »    PDF »
The Contribution of D-Mannose, L-Fucose, N-Acetylglucosamine, and Selectin Residues on the Binding of Glycodelin Isoforms to Human Spermatozoa.
P.C.N. Chiu, H.Y. Tsang, R. Koistinen, H. Koistinen, M. Seppala, K.F. Lee, and W.S.B. Yeung (2004)
Biol Reprod 70, 1710-1719
   Abstract »    Full Text »    PDF »
Sperm from {beta}1,4-galactosyltransferase I-null mice exhibit precocious capacitation.
C. Rodeheffer and B. D. Shur (2004)
Development 131, 491-501
   Abstract »    Full Text »    PDF »
Characterization of a novel ZP3-independent sperm-binding ligand that facilitates sperm adhesion to the egg coat.
C. Rodeheffer and B. D. Shur (2004)
Development 131, 503-512
   Abstract »    Full Text »    PDF »
Mutational analysis of the cytoplasmic domain of {beta}1,4-galactosyltransferase I: influence of phosphorylation on cell surface expression.
H. J. Hathaway, S. C. Evans, D. H. Dubois, C. I. Foote, B. H. Elder, and B. D. Shur (2003)
J. Cell Sci. 116, 4319-4330
   Abstract »    Full Text »    PDF »
Cell Adhesion and Fertilization: Steps in Oocyte Transport, Sperm-Zona Pellucida Interactions, and Sperm-Egg Fusion.
P. Talbot, B. D. Shur, and D. G. Myles (2003)
Biol Reprod 68, 1-9
   Abstract »    Full Text »    PDF »
Functional interaction between the SSeCKS scaffolding protein and the cytoplasmic domain of {beta}1,4-galactosyltransferase.
M. J. Wassler, C. I. Foote, I. H. Gelman, and B. D. Shur (2002)
J. Cell Sci. 114, 2291-2300
   Abstract »    Full Text »    PDF »
Neurotoxic Mechanisms by Alzheimer's Disease-Linked N141I Mutant Presenilin 2.
Y. Hashimoto, T. Niikura, Y. Ito, Y. Kita, K. Terashita, and I. Nishimoto (2002)
J. Pharmacol. Exp. Ther. 300, 736-745
   Abstract »    Full Text »    PDF »
Molecular Basis of Mammalian Gamete Binding.
D. J. Miller, X. Shi, and H. Burkin (2002)
Recent Prog. Horm. Res. 57, 37-73
   Abstract »    Full Text »    PDF »
Distinct Membrane Fractions from Mouse Sperm Bind Different Zona Pellucida Glycoproteins.
C. D. Thaler and R. A. Cardullo (2002)
Biol Reprod 66, 65-69
   Abstract »    Full Text »
Cell surface beta-1,4-galactosyltransferase-I activates G protein-dependent exocytotic signaling.
X Shi, S Amindari, K Paruchuru, D Skalla, H Burkin, B. Shur, and D. Miller (2001)
Development 128, 645-654
   Abstract »    PDF »
Expression and characterization of the human YWK-II gene, encoding a sperm membrane protein related to the Alzheimer {beta}A4-amyloid precursor protein.
P. Huang, S. Miao, H. Fan, Q. Sheng, Y. Yan, L. Wang, and S.S. Koide (2000)
Mol. Hum. Reprod. 6, 1069-1078
   Abstract »    Full Text »    PDF »
Expression of Carbohydrate Antigens in the Goat Uterus During Early Pregnancy and on Steroid-Treated Polarized Uterine Epithelial Cells In Vitro.
J.K. Powell, S.R. Glasser, S. Woldesenbet, R.C. Burghardt, and G.R. Newton (2000)
Biol Reprod 62, 277-284
   Abstract »    Full Text »
Multiple Domains Interacting with Gs in the Porcine Calcitonin Receptor.
P. Orcel, H. Tajima, Y. Murayama, T. Fujita, S. M. Krane, E. Ogata, S. R. Goldring, and I. Nishimoto (2000)
Mol. Endocrinol. 14, 170-182
   Abstract »    Full Text »
Clustering of cell surface (beta)1,4-galactosyltransferase I induces transient tyrosine phosphorylation of focal adhesion kinase and loss of stress fibers.
M. Wassler and B. Shur (2000)
J. Cell Sci. 113, 237-245
   Abstract »    PDF »
Ion Channels in Sperm Physiology.
A. Darszon, P. Labarca, T. Nishigaki, and F. Espinosa (1999)
Physiol Rev 79, 481-510
   Abstract »    Full Text »    PDF »
Maintenance of Motility in Mouse Sperm Permeabilized with Streptolysin O.
L. R. Johnson, S. B. Moss, and G. L. Gerton (1999)
Biol Reprod 60, 683-690
   Abstract »    Full Text »
Characterization of Domains in Mice of Calnexin-t, a Putative Molecular Chaperone Required in Sperm Fertility, with Use of Glutathione S-Transferase-Fusion Proteins.
S. Ohsako, L. Janulis, Y. Hayashi, and D. Bunick (1998)
Biol Reprod 59, 1214-1223
   Abstract »    Full Text »
Signal Transduction Mediated by the Truncated trkB Receptor Isoforms, trkB.T1 and trkB.T2.
G. T. Baxter, M. J. Radeke, R. C. Kuo, V. Makrides, B. Hinkle, R. Hoang, A. Medina-Selby, D. Coit, P. Valenzuela, and S. C. Feinstein (1997)
J. Neurosci. 17, 2683-2690
   Abstract »    Full Text »    PDF »
Insulin-induced Activation of NADPH-dependent H2O2 Generation in Human Adipocyte Plasma Membranes Is Mediated by Galpha i2.
H. I. Krieger-Brauer, P. K. Medda, and H. Kather (1997)
J. Biol. Chem. 272, 10135-10143
   Abstract »    Full Text »    PDF »
Sperm from beta 1,4-galactosyltransferase-null mice are refractory to ZP3-induced acrosome reactions and penetrate the zona pellucida poorly.
Q Lu and B. Shur (1997)
Development 124, 4121-4131
   Abstract »    PDF »
Mammary gland morphogenesis is inhibited in transgenic mice that overexpress cell surface beta1,4-galactosyltransferase.
H. Hathaway and B. Shur (1996)
Development 122, 2859-2872
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)