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Science 15 September 1995:
Vol. 269. no. 5230, pp. 1572 - 1575
DOI: 10.1126/science.7667635
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Articles

Science, Vol 269, Issue 5230, 1572-1575
Copyright © 1995 by American Association for the Advancement of Science

articles

Association of the yeast pheromone response G protein beta gamma subunits with the MAP kinase scaffold Ste5p

MS Whiteway, C Wu, T Leeuw, K Clark, A Fourest-Lieuvin, DY Thomas, and E Leberer

Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec.

The mating response pathway of the yeast Saccharomyces cerevisiae includes a heterotrimeric guanine nucleotide-binding protein (G protein) that activates a mitogen-activated protein MAP kinase cascade by an unknown mechanism. An amino-terminal fragment of the MAP kinase scaffold protein Ste5p that interfered with pheromone-induced cell cycle arrest was identified. A haploid-specific interaction between the amino terminus of Ste5p and the G protein beta subunit Ste4p was also detected in a two-hybrid assay, and the product of a signaling-defective allele of STE4 was defective in this interaction. In cells with a constitutively activated pheromone response pathway, epitope-tagged Ste4p was coimmunoprecipitated with Ste5p. Thus, association of the G protein and the MAP kinase cassette via the scaffolding protein Ste5p may transmit the G protein signal.


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