Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 15 September 1995:
Vol. 269. no. 5230, pp. 1567 - 1569
DOI: 10.1126/science.7545311
Prev | Table of Contents | Next

Articles

Science, Vol 269, Issue 5230, 1567-1569
Copyright © 1995 by American Association for the Advancement of Science

articles

Requirement for Src family protein tyrosine kinases in G2 for fibroblast cell division

S Roche, S Fumagalli, and SA Courtneidge

Institut National de la Sante et de la Recherche Medicale (INSERM) faculte de Pharmacie, Montpellier, France.

The protein tyrosine kinase c-Src is transiently activated at the transition from the G2 phase to mitosis in the cell cycle of mammalian fibroblasts. Fyn and Yes, the other members of the Src family present in fibroblasts, were also found to be activated at mitosis. In cells microinjected with a neutralizing antibody specific for Src, Fyn, and Yes (anti-cst.1) during G2, cell division was inhibited by 75 percent. The block occurred before nuclear envelope breakdown. Antibodies specific for phosphatidylinositol-3 kinase alpha and phospholipase C-gamma 1 had no effect. Microinjection of the Src homology 2 (SH2) domain of Fyn was also inhibitory. Functional redundancy between members of the Src family was observed; a Src-specific antibody had no effect in NIH 3T3 cells but inhibited cell division in fibroblasts in which the only functional Src family kinase was Src itself. Thus, Src family kinases and proteins associating with their SH2 domains are required for entry into mitosis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Inhibition of Src Family Kinases with Dasatinib Blocks Migration and Invasion of Human Melanoma Cells.
R. Buettner, T. Mesa, A. Vultur, F. Lee, and R. Jove (2008)
Mol. Cancer Res. 6, 1766-1774
   Abstract »    Full Text »    PDF »
Potential Molecular Mechanism for c-Src Kinase-mediated Regulation of Intestinal Cell Migration.
S. Mathew, S. P. George, Y. Wang, M. R. Siddiqui, K. Srinivasan, L. Tan, and S. Khurana (2008)
J. Biol. Chem. 283, 22709-22722
   Abstract »    Full Text »    PDF »
The membrane targeting and spatial activation of Src, Yes and Fyn is influenced by palmitoylation and distinct RhoB/RhoD endosome requirements.
E. Sandilands, V. G. Brunton, and M. C. Frame (2007)
J. Cell Sci. 120, 2555-2564
   Abstract »    Full Text »    PDF »
Src Signaling Regulates Completion of Abscission in Cytokinesis through ERK/MAPK Activation at the Midbody.
K. Kasahara, Y. Nakayama, Y. Nakazato, K. Ikeda, T. Kuga, and N. Yamaguchi (2007)
J. Biol. Chem. 282, 5327-5339
   Abstract »    Full Text »    PDF »
Identification of a Filamin Docking Site on PTP-PEST.
M. P. Playford, P. D. Lyons, S. K. Sastry, and M. D. Schaller (2006)
J. Biol. Chem. 281, 34104-34112
   Abstract »    Full Text »    PDF »
K-ras Asp12 mutant neither interacts with Raf, nor signals through Erk and is less tumorigenic than K-ras Val12.
M. V. Cespedes, F. J. Sancho, S. Guerrero, M. Parreno, I. Casanova, M. A. Pavon, E. Marcuello, M. Trias, M. Cascante, G. Capella, et al. (2006)
Carcinogenesis 27, 2190-2200
   Abstract »    Full Text »    PDF »
Role of Src homology 2 domain-mediated PTK signaling in mouse zygotic development..
L. Meng, J. Luo, C. Li, and W. H Kinsey (2006)
Reproduction 132, 413-421
   Abstract »    Full Text »    PDF »
The Src Family Kinase c-Yes Is Required for Maturation of West Nile Virus Particles.
A. J. Hirsch, G. R. Medigeshi, H. L. Meyers, V. DeFilippis, K. Fruh, T. Briese, W. I. Lipkin, and J. A. Nelson (2005)
J. Virol. 79, 11943-11951
   Abstract »    Full Text »    PDF »
Cardioprotection with palm tocotrienol: antioxidant activity of tocotrienol is linked with its ability to stabilize proteasomes.
S. Das, S. R. Powell, P. Wang, A. Divald, K. Nesaretnam, A. Tosaki, G. A. Cordis, N. Maulik, and D. K. Das (2005)
Am J Physiol Heart Circ Physiol 289, H361-H367
   Abstract »    Full Text »    PDF »
SH2 domain-mediated activation of an SRC family kinase is not required to initiate Ca2+ release at fertilization in mouse eggs.
L. M Mehlmann and L. A Jaffe (2005)
Reproduction 129, 557-564
   Abstract »    Full Text »    PDF »
Distinct roles for multiple Src family kinases at fertilization.
F. J. O'Neill, J. Gillett, and K. R. Foltz (2004)
J. Cell Sci. 117, 6227-6238
   Abstract »    Full Text »    PDF »
Fyn kinase-tubulin interaction during meiosis of rat eggs.
A Talmor-Cohen, R Tomashov-Matar, W B Tsai, W H Kinsey, and R Shalgi (2004)
Reproduction 128, 387-393
   Abstract »    Full Text »    PDF »
Inhibition of JNK2 Disrupts Anaphase and Produces Aneuploidy in Mammalian Cells.
R. A. MacCorkle and T.-H. Tan (2004)
J. Biol. Chem. 279, 40112-40121
   Abstract »    Full Text »    PDF »
C-Src Tyrosine Kinase Activity Is Associated with Tumor Colonization in Bone and Lung in an Animal Model of Human Breast Cancer Metastasis.
A. Myoui, R. Nishimura, P. J. Williams, T. Hiraga, D. Tamura, T. Michigami, G. R. Mundy, and T. Yoneda (2003)
Cancer Res. 63, 5028-5033
   Abstract »    Full Text »    PDF »
Activation of Src Kinase in Platelet-Derived Growth Factor-B-Dependent Tubular Regeneration after Acute Ischemic Renal Injury.
M. Takikita-Suzuki, M. Haneda, M. Sasahara, M. K. Owada, T. Nakagawa, M. Isono, S. Takikita, D. Koya, K. Ogasawara, and R. Kikkawa (2003)
Am. J. Pathol. 163, 277-286
   Abstract »    Full Text »    PDF »
The SH4-Unique-SH3-SH2 domains dictate specificity in signaling that differentiate c-Yes from c-Src.
J. M. Summy, Y. Qian, B.-H. Jiang, A. Guappone-Koay, A. Gatesman, X. Shi, and D. C. Flynn (2003)
J. Cell Sci. 116, 2585-2598
   Abstract »    Full Text »    PDF »
c-Src Regulation of Fibroblast Growth Factor-induced Proliferation in Murine Embryonic Fibroblasts.
D. M. Kilkenny, J. V. Rocheleau, J. Price, M. B. Reich, and G. G. Miller (2003)
J. Biol. Chem. 278, 17448-17454
   Abstract »    Full Text »    PDF »
Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes.
M. Baumgartner, P. Angelisova, N. Setterblad, N. Mooney, D. Werling, V. Horejsi, and G. Langsley (2003)
Blood 101, 1874-1881
   Abstract »    Full Text »    PDF »
Microtubule involvement in NIH 3T3 Golgi and MTOC polarity establishment.
J. Magdalena, T. H. Millard, and L. M. Machesky (2003)
J. Cell Sci. 116, 743-756
   Abstract »    Full Text »    PDF »
pp60c-Src Kinase Mediates Growth Effects of the Full-Length Precursor Progastrin1-80 Peptide on Rat Intestinal Epithelial Cells, in Vitro.
D. Brown, U. Yallampalli, A. Owlia, and P. Singh (2003)
Endocrinology 144, 201-211
   Abstract »    Full Text »    PDF »
Galpha and Gbeta gamma Require Distinct Src-dependent Pathways to Activate Rap1 and Ras.
J. M. Schmitt and P. J. S. Stork (2002)
J. Biol. Chem. 277, 43024-43032
   Abstract »    Full Text »    PDF »
Distinct Cell Cycle Timing Requirements for Extracellular Signal-Regulated Kinase and Phosphoinositide 3-Kinase Signaling Pathways in Somatic Cell Mitosis.
E. C. Roberts, P. S. Shapiro, T. S. Nahreini, G. Pages, J. Pouyssegur, and N. G. Ahn (2002)
Mol. Cell. Biol. 22, 7226-7241
   Abstract »    Full Text »    PDF »
Tyrosine-Phosphorylated Extracellular Signal-Regulated Kinase Associates with the Golgi Complex during G2/M Phase of the Cell Cycle: Evidence for Regulation of Golgi Structure.
H. Cha and P. Shapiro (2001)
J. Cell Biol. 153, 1355-1368
   Abstract »    Full Text »    PDF »
Overexpression of the Csk homologous kinase (Chk tyrosine kinase) induces multinucleation: a possible role for chromosome-associated Chk in chromosome dynamics.
N Yamaguchi, Y Nakayama, T Urakami, S Suzuki, T Nakamura, T Suda, and N Oku (2001)
J. Cell Sci. 114, 1631-1641
   Abstract »    PDF »
Structure, Function, and Control of Phosphoinositide-Specific Phospholipase C.
M. J. Rebecchi and S. N. Pentyala (2000)
Physiol Rev 80, 1291-1335
   Abstract »    Full Text »    PDF »
Src Family Kinase Activity Is Required for Kit-mediated Mitogen-activated Protein (MAP) Kinase Activation, However Loss of Functional Retinoblastoma Protein Makes MAP Kinase Activation Unnecessary for Growth of Small Cell Lung Cancer Cells.
C. Bondzi, J. Litz, P. Dent, and G. W. Krystal (2000)
Cell Growth Differ. 11, 305-314
   Abstract »    Full Text »
Slap Negatively Regulates Src Mitogenic Function but Does Not Revert Src-Induced Cell Morphology Changes.
G. Manes, P. Bello, and S. Roche (2000)
Mol. Cell. Biol. 20, 3396-3406
   Abstract »    Full Text »
Overexpression of C-terminal Src Kinase Blocks 14,15-Epoxyeicosatrienoic Acid-induced Tyrosine Phosphorylation and Mitogenesis.
J.-K. Chen, J. Capdevila, and R. C. Harris (2000)
J. Biol. Chem. 275, 13789-13792
   Abstract »    Full Text »    PDF »
Transcription of the Human c-Src Promoter Is Dependent on Sp1, a Novel Pyrimidine Binding Factor SPy, and Can Be Inhibited by Triplex-forming Oligonucleotides.
S. Ritchie, F. M. Boyd, J. Wong, and K. Bonham (2000)
J. Biol. Chem. 275, 847-854
   Abstract »    Full Text »    PDF »
Sperm extract injection into ascidian eggs signals Ca(2+) release by the same pathway as fertilization.
L. Runft and L. Jaffe (2000)
Development 127, 3227-3236
   Abstract »    PDF »
MAPK Mediates RAS-induced Chromosome Instability.
H. I. Saavedra, K. Fukasawa, C. W. Conn, and P. J. Stambrook (1999)
J. Biol. Chem. 274, 38083-38090
   Abstract »    Full Text »    PDF »
Inhibition of Src Kinases by a Selective Tyrosine Kinase Inhibitor Causes Mitotic Arrest.
M. M. Moasser, M. Srethapakdi, K. S. Sachar, A. J. Kraker, and N. Rosen (1999)
Cancer Res. 59, 6145-6152
   Abstract »    Full Text »    PDF »
Ubiquitin-mediated degradation of active Src tyrosine kinase.
K. F. Harris, I. Shoji, E. M. Cooper, S. Kumar, H. Oda, and P. M. Howley (1999)
PNAS 96, 13738-13743
   Abstract »    Full Text »    PDF »
Platelet-derived Growth Factor Receptor-induced Feed-forward Inhibition of Excitatory Transmission between Hippocampal Pyramidal Neurons.
S. Lei, W.-Y. Lu, Z.-G. Xiong, B. A. Orser, C. F. Valenzuela, and J. F. MacDonald (1999)
J. Biol. Chem. 274, 30617-30623
   Abstract »    Full Text »    PDF »
Requirement of a Src Family Kinase for Initiating Calcium Release at Fertilization in Starfish Eggs.
A. F. Giusti, D. J. Carroll, Y. A. Abassi, M. Terasaki, K. R. Foltz, and L. A. Jaffe (1999)
J. Biol. Chem. 274, 29318-29322
   Abstract »    Full Text »    PDF »
Src, a molecular switch governing gain control of synaptic transmission mediated by N-methyl-D-aspartate receptors.
X.-M. Yu and M. W. Salter (1999)
PNAS 96, 7697-7704
   Abstract »    Full Text »    PDF »
Characterization of Sam68-like mammalian proteins SLM-1 and SLM-2: SLM-1 is a Src substrate during mitosis.
M. Di Fruscio, T. Chen, and S. Richard (1999)
PNAS 96, 2710-2715
   Abstract »    Full Text »    PDF »
Nuclear Localization of Mitogen-activated Protein Kinase Kinase 1 (MKK1) Is Promoted by Serum Stimulation and G2-M Progression. REQUIREMENT FOR PHOSPHORYLATION AT THE ACTIVATION LIP AND SIGNALING DOWNSTREAM OF MKK.
N. S. Tolwinski, P. S. Shapiro, S. Goueli, and N. G. Ahn (1999)
J. Biol. Chem. 274, 6168-6174
   Abstract »    Full Text »    PDF »
Characterization of Raf-1 Activation in Mitosis.
A. D. Laird, D. K. Morrison, and D. Shalloway (1999)
J. Biol. Chem. 274, 4430-4439
   Abstract »    Full Text »    PDF »
Complementation of Defective Colony-Stimulating Factor 1 Receptor Signaling and Mitogenesis by Raf and v-Src.
N. Aziz, H. Cherwinski, and M. McMahon (1999)
Mol. Cell. Biol. 19, 1101-1115
   Abstract »    Full Text »    PDF »
Src-Related Protein Tyrosine Kinases in Hematopoiesis.
S. J. Corey and S. M. Anderson (1999)
Blood 93, 1-14
   Full Text »    PDF »
Cell-cycle arrest and apoptosis hypersusceptibility as a consequence of Lck deficiency in nontransformed T lymphocytes.
B. K. al-Ramadi, H. Zhang, and A. L. M. Bothwell (1998)
PNAS 95, 12498-12503
   Abstract »    Full Text »    PDF »
Active MAP Kinase in Mitosis: Localization at Kinetochores and Association with the Motor Protein CENP-E.
M. Zecevic, A. D. Catling, S. T. Eblen, L. Renzi, J. C. Hittle, T. J. Yen, G. J. Gorbsky, and M. J. Weber (1998)
J. Cell Biol. 142, 1547-1558
   Abstract »    Full Text »    PDF »
Activation of the MKK/ERK Pathway during Somatic Cell Mitosis: Direct Interactions of Active ERK with Kinetochores and Regulation of the Mitotic 3F3/2 Phosphoantigen.
P. S. Shapiro, E. Vaisberg, A. J. Hunt, N. S. Tolwinski, A. M. Whalen, J. R. McIntosh, and N. G. Ahn (1998)
J. Cell Biol. 142, 1533-1545
   Abstract »    Full Text »    PDF »
Mechanisms of Ligand-Induced Aryl Hydrocarbon Receptor-Mediated Biochemical and Toxic Responses.
C. L. Wilson and S. Safe (1998)
Toxicol Pathol 26, 657-671
   Abstract »    PDF »
Interaction of p59fyn Kinase with the Dynein Light Chain, Tctex-1, and Colocalization During Cytokinesis.
K. S. Campbell, S. Cooper, M. Dessing, S. Yates, and A. Buder (1998)
J. Immunol. 161, 1728-1737
   Abstract »    Full Text »    PDF »
Src Family Tyrosine Kinase Regulates Intracellular pH in Cardiomyocytes.
M. Puceat, S. Roche, and G. Vassort (1998)
J. Cell Biol. 141, 1637-1646
   Abstract »    Full Text »    PDF »
Expression of the pRb-Binding Regions of E1A Enables Efficient Transformation of Primary Epithelial Cells by v-src.
R. S. Fischer and M. P. Quinlan (1998)
J. Virol. 72, 2815-2824
   Abstract »    Full Text »    PDF »
Activation of Src Family Members Is Not Required for the Platelet-Derived Growth Factor beta  Receptor To Initiate Mitogenesis.
K. A. DeMali and A. Kazlauskas (1998)
Mol. Cell. Biol. 18, 2014-2022
   Abstract »    Full Text »
A Role for Src in Signal Relay by the Platelet-derived Growth Factor alpha  Receptor.
J. A. Gelderloos, S. Rosenkranz, C. Bazenet, and A. Kazlauskas (1998)
J. Biol. Chem. 273, 5908-5915
   Abstract »    Full Text »    PDF »
Src64 is required for ovarian ring canal morphogenesis during Drosophila oogenesis.
G. Dodson, D. Guarnieri, and M. Simon (1998)
Development 125, 2883-2892
   Abstract »    PDF »
The Platelet-derived Growth Factor beta  Receptor Triggers Multiple Cytoplasmic Signaling Cascades That Arrive at the Nucleus as Distinguishable Inputs.
J.-P. Montmayeur, M. Valius, J. Vandenheede, and A. Kazlauskas (1997)
J. Biol. Chem. 272, 32670-32678
   Abstract »    Full Text »    PDF »
Tyrosine Phosphorylation Modulates Current Amplitude and Kinetics of a Neuronal Voltage-Gated Potassium Channel.
D. A. Fadool, T. C. Holmes, K. Berman, D. Dagan, and I. B. Levitan (1997)
J Neurophysiol 78, 1563-1573
   Abstract »    Full Text »    PDF »
Mitochondrial Proliferation and Paradoxical Membrane Depolarization during Terminal Differentiation and Apoptosis in a Human Colon Carcinoma Cell Line.
M. Mancini, B. O. Anderson, E. Caldwell, M. Sedghinasab, P. B. Paty, and D. M. Hockenbery (1997)
J. Cell Biol. 138, 449-469
   Abstract »    Full Text »    PDF »
A Role for Sam68 in Cell Cycle Progression Antagonized by a Spliced Variant within the KH Domain.
I. Barlat, F. Maurier, M. Duchesne, E. Guitard, B. Tocque, and F. Schweighoffer (1997)
J. Biol. Chem. 272, 3129-3132
   Abstract »    Full Text »    PDF »
Upregulation of Cortactin Expression During the Maturation of Megakaryocytes.
X. Zhan, C. C. Haudenschild, Y. Ni, E. Smith, and C. Huang (1997)
Blood 89, 457-464
   Abstract »    Full Text »    PDF »
Molecular Interaction between Limb Deformity Proteins (Formins) and Src Family Kinases.
P. Uetz, S. Fumagalli, D. James, and R. Zeller (1996)
J. Biol. Chem. 271, 33525-33530
   Abstract »    Full Text »    PDF »
Requirement for c-Src Catalytic Activity and the SH3 Domain in Platelet-derived Growth Factor BB and Epidermal Growth Factor Mitogenic Signaling.
M. A. Broome and T. Hunter (1996)
J. Biol. Chem. 271, 16798-16806
   Abstract »    Full Text »    PDF »
Identification of a Carboxyl-terminal Diaphanous-related Formin Homology Protein Autoregulatory Domain.
A. S. Alberts (2001)
J. Biol. Chem. 276, 2824-2830
   Abstract »    Full Text »    PDF »
Functional Analysis of Csk and CHK Kinases in Breast Cancer Cells.
C. Bougeret, S. Jiang, I. Keydar, and H. Avraham (2001)
J. Biol. Chem. 276, 33711-33720
   Abstract »    Full Text »    PDF »
`Srcasm: a Novel SrcActivating and Signaling Molecule.
J. T. Seykora, L. Mei, G. P. Dotto, and P. L. Stein (2002)
J. Biol. Chem. 277, 2812-2822
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)