Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 1 September 1995:
Vol. 269. no. 5228, pp. 1273 - 1278
DOI: 10.1126/science.7544493
Prev | Table of Contents | Next

Articles

Science, Vol 269, Issue 5228, 1273-1278
Copyright © 1995 by American Association for the Advancement of Science

articles

Conformation and function of the N-linked glycan in the adhesion domain of human CD2

DF Wyss, JS Choi, J Li, MH Knoppers, KJ Willis, AR Arulanandam, A Smolyar, EL Reinherz, and G Wagner

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.

The adhesion domain of human CD2 bears a single N-linked carbohydrate. The solution structure of a fragment of CD2 containing the covalently bound high-mannose N-glycan [-(N-acetylglucosamine)2-(mannose)5-8] was solved by nuclear magnetic resonance. The stem and two of three branches of the carbohydrate structure are well defined and the mobility of proximal glycan residues is restricted. Mutagenesis of all residues in the vicinity of the glycan suggests that the glycan is not a component of the CD2-CD58 interface; rather, the carbohydrate stabilizes the protein fold by counterbalancing an unfavorable clustering of five positive charges centered about lysine-61 of CD2.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Proximal Glycans Outside of the Epitopes Regulate the Presentation of HIV-1 Envelope gp120 Helper Epitopes.
H. Li, C.-F. Xu, S. Blais, Q. Wan, H.-T. Zhang, S. J. Landry, and C. E. Hioe (2009)
J. Immunol. 182, 6369-6378
   Abstract »    Full Text »    PDF »
The core trisaccharide of an N-linked glycoprotein intrinsically accelerates folding and enhances stability.
S. R. Hanson, E. K. Culyba, T.-L. Hsu, C.-H. Wong, J. W. Kelly, and E. T. Powers (2009)
PNAS 106, 3131-3136
   Abstract »    Full Text »    PDF »
Effect of glycosylation on protein folding: A close look at thermodynamic stabilization.
D. Shental-Bechor and Y. Levy (2008)
PNAS 105, 8256-8261
   Abstract »    Full Text »    PDF »
Identification of N-Glycosylated Proteins from the Central Nervous System of Drosophila Melanogaster.
K. Koles, J.-M. Lim, K. Aoki, M. Porterfield, M. Tiemeyer, L. Wells, and V. Panin (2007)
Glycobiology 17, 1388-1403
   Abstract »    Full Text »    PDF »
The Contribution of Conformational Adjustments and Long-range Electrostatic Forces to the CD2/CD58 Interaction.
A. Kearney, A. Avramovic, M. A. A. Castro, A. M. Carmo, S. J. Davis, and P. A. van der Merwe (2007)
J. Biol. Chem. 282, 13160-13166
   Abstract »    Full Text »    PDF »
Observation of a unique pattern of bifurcated hydrogen bonds in the crystal structures of the N-glycoprotein linkage region models.
D. Loganathan and U. Aich (2006)
Glycobiology 16, 343-348
   Abstract »    Full Text »    PDF »
Core Fucosylation Regulates Epidermal Growth Factor Receptor-mediated Intracellular Signaling.
X. Wang, J. Gu, H. Ihara, E. Miyoshi, K. Honke, and N. Taniguchi (2006)
J. Biol. Chem. 281, 2572-2577
   Abstract »    Full Text »    PDF »
Ribosome display of mammalian receptor domains.
B. Schimmele, N. Grafe, and A. Pluckthun (2005)
Protein Eng. Des. Sel. 18, 285-294
   Abstract »    Full Text »    PDF »
Contribution of N-Linked Glycans to the Conformation and Function of Intercellular Adhesion Molecules (ICAMs).
D. Jimenez, P. Roda-Navarro, T. A. Springer, and J. M. Casasnovas (2005)
J. Biol. Chem. 280, 5854-5861
   Abstract »    Full Text »    PDF »
Solution structure of the CD3{epsilon}{delta} ectodomain and comparison with CD3{epsilon}{gamma} as a basis for modeling T cell receptor topology and signaling.
Z.-Y. J. Sun, S. T. Kim, I. C. Kim, A. Fahmy, E. L. Reinherz, and G. Wagner (2004)
PNAS 101, 16867-16872
   Abstract »    Full Text »    PDF »
The Interactions of Allium sativum Leaf Agglutinin with a Chaperonin Group of Unique Receptor Protein Isolated from a Bacterial Endosymbiont of the Mustard Aphid.
S. Banerjee, D. Hess, P. Majumder, D. Roy, and S. Das (2004)
J. Biol. Chem. 279, 23782-23789
   Abstract »    Full Text »    PDF »
Effect of N-linked glycosylation on the aspartic proteinase porcine pepsin expressed from Pichia pastoris.
M. A. Yoshimasu, T. Tanaka, J.-K. Ahn, and R. Y. Yada (2004)
Glycobiology 14, 417-429
   Abstract »    Full Text »    PDF »
Statistical analysis of the protein environment of N-glycosylation sites: implications for occupancy, structure, and folding.
A.-J. Petrescu, A.-L. Milac, S. M. Petrescu, R. A. Dwek, and M. R. Wormald (2004)
Glycobiology 14, 103-114
   Abstract »    Full Text »    PDF »
New Antiproliferative Immunosuppressive Agents.
T. M. Sievers (2003)
Journal of Pharmacy Practice 16, 401-413
   Abstract »    PDF »
HLA-F Surface Expression on B Cell and Monocyte Cell Lines Is Partially Independent from Tapasin and Completely Independent from TAP.
N. Lee and D. E. Geraghty (2003)
J. Immunol. 171, 5264-5271
   Abstract »    Full Text »    PDF »
The contribution of N-glycans and their processing in the endoplasmic reticulum to glycoprotein biosynthesis.
E. S. Trombetta (2003)
Glycobiology 13, 77R-91R
   Abstract »    Full Text »    PDF »
Stabilizing the open conformation of the integrin headpiece with a glycan wedge increases affinity for ligand.
B.-H. Luo, T. A. Springer, and J. Takagi (2003)
PNAS 100, 2403-2408
   Abstract »    Full Text »    PDF »
Post-translational Modification of Bone Morphogenetic Protein-1 Is Required for Secretion and Stability of the Protein.
L. Garrigue-Antar, N. Hartigan, and K. E. Kadler (2002)
J. Biol. Chem. 277, 43327-43334
   Abstract »    Full Text »    PDF »
The Broadly Neutralizing Anti-Human Immunodeficiency Virus Type 1 Antibody 2G12 Recognizes a Cluster of {alpha}1->2 Mannose Residues on the Outer Face of gp120.
C. N. Scanlan, R. Pantophlet, M. R. Wormald, E. Ollmann Saphire, R. Stanfield, I. A. Wilson, H. Katinger, R. A. Dwek, P. M. Rudd, and D. R. Burton (2002)
J. Virol. 76, 7306-7321
   Abstract »    Full Text »    PDF »
Metal binding affinity and structural properties of an isolated EF-loop in a scaffold protein.
Y. Ye, H.-W. Lee, W. Yang, S. J. Shealy, A. L. Wilkins, Z.-r. Liu, I. Torshin, R. Harrison, R. Wohlhueter, and J. J. Yang (2001)
Protein Eng. Des. Sel. 14, 1001-1013
   Abstract »    Full Text »    PDF »
The Functional Binding Site for the C-Type Lectin-Like Natural Killer Cell Receptor Ly49a Spans Three Domains of Its Major Histocompatibility Complex Class I Ligand.
N. Matsumoto, M. Mitsuki, K. Tajima, W. M. Yokoyama, and K. Yamamoto (2001)
J. Exp. Med. 193, 147-158
   Abstract »    Full Text »    PDF »
Homologue Scanning Mutagenesis Reveals Cd66 Receptor Residues Required for Neisserial Opa Protein Binding.
M. P. Bos, D. Hogan, and R. J. Belland (1999)
J. Exp. Med. 190, 331-340
   Abstract »    Full Text »    PDF »
Probing cell-surface architecture through synthesis: An NMR-determined structural motif for tumor-associated mucins.
D. H. Live, L. J. Williams, S. D. Kuduk, J. B. Schwarz, P. W. Glunz, X.-T. Chen, D. Sames, R. A. Kumar, and S. J. Danishefsky (1999)
PNAS 96, 3489-3493
   Abstract »    Full Text »    PDF »
Lysine-based Structure Responsible for Selective Mannose Phosphorylation of Cathepsin D and Cathepsin L Defines a Common Structural Motif for Lysosomal Enzyme Targeting.
J. W. Cuozzo, K. Tao, M. Cygler, J. S. Mort, and G. G. Sahagian (1998)
J. Biol. Chem. 273, 21067-21076
   Abstract »    Full Text »    PDF »
Glycosylation Provides Both Stimulatory and Inhibitory Effects on Cell Surface and Soluble CD44 Binding to Hyaluronan.
T. P. Skelton, C. Zeng, A. Nocks, and I. Stamenkovic (1998)
J. Cell Biol. 140, 431-446
   Abstract »    Full Text »    PDF »
Molecular Characterization and Functional Analysis of Murine Interleukin 4 Receptor Allotypes.
T. Schulte, R. Kurrle, M. Rollinghoff, and A. Gessner (1997)
J. Exp. Med. 186, 1419-1429
   Abstract »    Full Text »    PDF »
The Recognition of the Nonclassical Major Histocompatibility Complex (MHC) Class I Molecule, T10, by the {gamma}{delta} T Cell, G8.
M. P. Crowley, Z. Reich, N. Mavaddat, J. D. Altman, and Y.-h. Chien (1997)
J. Exp. Med. 185, 1223-1230
   Abstract »    Full Text »    PDF »
Apical sorting of hepatitis B surface antigen (HBsAg) is independent of N-glycosylation and glycosylphosphatidylinositol-anchored protein segregation.
M. P. Marzolo, P. Bull, and A. Gonzalez (1997)
PNAS 94, 1834-1839
   Abstract »    Full Text »    PDF »
Conformational influences of glycosylation of a peptide: A possible model for the effect of glycosylation on the rate of protein folding.
D. H. Live, R. A. Kumar, X. Beebe, and S. J. Danishefsky (1996)
PNAS 93, 12759-12761
   Abstract »    Full Text »    PDF »
Response: CD2: An Exception to the Immunologlobulin Superfamily Concept?.
E. L. Reinherz, J. Li, A. Smoylar, D. F. Wyss, M. H. Knoppers, K. J. Willis, A. R. N. Arulanandam, J. S. Choi, and G. Wagner (1996)
Science 273, 1242
   PDF »
A Single N-linked Glycosylation Site Is Implicated in the Regulation of Ligand Recognition by the I-type Lectins CD22 and CD33.
D. Sgroi, A. Nocks, and I. Stamenkovic (1996)
J. Biol. Chem. 271, 18803-18809
   Abstract »    Full Text »    PDF »
Aberrant Glycosylation of E-cadherin Enhances Cell-Cell Binding to Suppress Metastasis.
M. Yoshimura, Y. Ihara, Y. Matsuzawa, and N. Taniguchi (1996)
J. Biol. Chem. 271, 13811-13815
   Abstract »    Full Text »    PDF »
Glycobiology: more functions for oligosaccharides.
R. Dwek (1995)
Science 269, 1234-1235
   PDF »
Roles of Individual N-Glycans for ATP Potency and Expression of the Rat P2X1 Receptor.
J. Rettinger, A. Aschrafi, and G. Schmalzing (2000)
J. Biol. Chem. 275, 33542-33547
   Abstract »    Full Text »    PDF »
The Addition of Bisecting N-Acetylglucosamine Residues to E-cadherin Down-regulates the Tyrosine Phosphorylation of beta -Catenin.
T. Kitada, E. Miyoshi, K. Noda, S. Higashiyama, H. Ihara, N. Matsuura, N. Hayashi, S. Kawata, Y. Matsuzawa, and N. Taniguchi (2001)
J. Biol. Chem. 276, 475-480
   Abstract »    Full Text »    PDF »
Overexpression of N-Acetylglucosaminyltransferase III Enhances the Epidermal Growth Factor-induced Phosphorylation of ERK in HeLaS3 Cells by Up-regulation of the Internalization Rate of the Receptors.
Y. Sato, M. Takahashi, Y. Shibukawa, S. K. Jain, R. Hamaoka, J.-i. Miyagawa, Y. Yaginuma, K. Honke, M. Ishikawa, and N. Taniguchi (2001)
J. Biol. Chem. 276, 11956-11962
   Abstract »    Full Text »    PDF »
A space-time structure determination of human CD2 reveals the CD58-binding mode.
A. Kitao and G. Wagner (2000)
PNAS 97, 2064-2068
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)