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Science 4 August 1995:
Vol. 269. no. 5224, pp. 699 - 702
DOI: 10.1126/science.7624801
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Articles

Science, Vol 269, Issue 5224, 699-702
Copyright © 1995 by American Association for the Advancement of Science

articles

Excision of deoxyribose phosphate residues by DNA polymerase beta during DNA repair

Y Matsumoto and K Kim

Department of Radiation Oncology, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.

Eukaryotic DNA polymerase beta (pol beta) can catalyze DNA synthesis during base excision DNA repair. It is shown here that pol beta also catalyzes release of 5'-terminal deoxyribose phosphate (dRP) residues from incised apurinic-apyrimidinic sites, which are common intermediate products in base excision repair. The catalytic domain for this activity resides within an amino-terminal 8-kilodalton fragment of pol beta, which comprises a distinct structural domain of the enzyme. Magnesium is required for the release of dRP from double-stranded DNA but not from a single-stranded oligonucleotide. Analysis of the released products indicates that the excision reaction occurs by beta-elimination rather than hydrolysis.


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T. A. Ranalli, S. Tom, and R. A. Bambara (2002)
J. Biol. Chem. 277, 41715-41724
   Abstract »    Full Text »    PDF »
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T. T. Saxowsky, Y. Matsumoto, and P. T. Englund (2002)
J. Biol. Chem. 277, 37201-37206
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M. Maitra, A. Gudzelak Jr., S.-X. Li, Y. Matsumoto, K. A. Eckert, J. Jager, and J. B. Sweasy (2002)
J. Biol. Chem. 277, 35550-35560
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Direct Interaction between Mammalian DNA Polymerase beta and Proliferating Cell Nuclear Antigen.
P. S. Kedar, S.-J. Kim, A. Robertson, E. Hou, R. Prasad, J. K. Horton, and S. H. Wilson (2002)
J. Biol. Chem. 277, 31115-31123
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H. Budworth, I. I. Dianova, V. N. Podust, and G. L. Dianov (2002)
J. Biol. Chem. 277, 21300-21305
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Dynamics of Gapped DNA Recognition by Human Polymerase beta.
M. J. Jezewska, R. Galletto, and W. Bujalowski (2002)
J. Biol. Chem. 277, 20316-20327
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Mutations associated with base excision repair deficiency and methylation-induced genotoxic stress.
R. W. Sobol, D. E. Watson, J. Nakamura, F. M. Yakes, E. Hou, J. K. Horton, J. Ladapo, B. Van Houten, J. A. Swenberg, K. R. Tindall, et al. (2002)
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G. I. Belova, R. Prasad, I. V. Nazimov, S. H. Wilson, and A. I. Slesarev (2002)
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Y. Mizushina, S. Kamisuki, N. Kasai, N. Shimazaki, M. Takemura, H. Asahara, S. Linn, S. Yoshida, A. Matsukage, O. Koiwai, et al. (2002)
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Base Excision Repair Intermediates as Topoisomerase II Poisons.
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S. Taladriz, T. Hanke, M. J. Ramiro, M. Garcia-Diaz, M. Garcia de Lacoba, L. Blanco, and V. Larraga (2001)
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M. M. Hsieh, V. Hegde, M. R. Kelley, and W. A. Deutsch (2001)
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K. Bebenek, A. Tissier, E. G. Frank, J. P. McDonald, R. Prasad, S. H. Wilson, R. Woodgate, and T. A. Kunkel (2001)
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K. G. Pinz and D. F. Bogenhagen (2000)
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L. J. Deterding, R. Prasad, G. P. Mullen, S. H. Wilson, and K. B. Tomer (2000)
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5'-Nicked Apurinic/Apyrimidinic Sites Are Resistant to beta -Elimination by beta -Polymerase and Are Persistent in Human Cultured Cells after Oxidative Stress.
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J. Biol. Chem. 275, 5323-5328
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FEN1 Stimulation of DNA Polymerase beta Mediates an Excision Step in Mammalian Long Patch Base Excision Repair.
R. Prasad, G. L. Dianov, V. A. Bohr, and S. H. Wilson (2000)
J. Biol. Chem. 275, 4460-4466
   Abstract »    Full Text »    PDF »
Mapping Oxidative DNA Damage and Mechanisms of Repair.
S. A. AKMAN, T. R. O'CONNOR, and H. RODRIGUEZ (2000)
Ann. N.Y. Acad. Sci. 899, 88-102
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J. L. Kosa and J. B. Sweasy (1999)
J. Biol. Chem. 274, 35866-35872
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Quality Control by DNA Repair.
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Science 286, 1897-1905
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C. A. Clairmont, L. Narayanan, K.-W. Sun, P. M. Glazer, and J. B. Sweasy (1999)
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Endogenous Apurinic/Apyrimidinic Sites in Genomic DNA of Mammalian Tissues.
J. Nakamura and J. A. Swenberg (1999)
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E. Efrati, G. Tocco, R. Eritja, S. H. Wilson, and M. F. Goodman (1999)
J. Biol. Chem. 274, 15920-15926
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Role of DNA Polymerase beta  in the Excision Step of Long Patch Mammalian Base Excision Repair.
G. L. Dianov, R. Prasad, S. H. Wilson, and V. A. Bohr (1999)
J. Biol. Chem. 274, 13741-13743
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C. L. Afonso, E. R. Tulman, Z. Lu, E. Oma, G. F. Kutish, and D. L. Rock (1999)
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Repair Pathways for Processing of 8-Oxoguanine in DNA by Mammalian Cell Extracts.
G. Dianov, C. Bischoff, J. Piotrowski, and V. A. Bohr (1998)
J. Biol. Chem. 273, 33811-33816
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Dynamics of the Interaction of Human Apurinic Endonuclease (Ape1) with Its Substrate and Product.
Y. Masuda, R. A. O. Bennett, and B. Demple (1998)
J. Biol. Chem. 273, 30352-30359
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Excision of C-4'-oxidized Deoxyribose Lesions from Double-stranded DNA by Human Apurinic/Apyrimidinic Endonuclease (Ape1 Protein) and DNA Polymerase beta.
Y.-j. Xu, E. Y. Kim, and B. Demple (1998)
J. Biol. Chem. 273, 28837-28844
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Replication Protein A Stimulates Long Patch DNA Base Excision Repair.
M. S. DeMott, S. Zigman, and R. A. Bambara (1998)
J. Biol. Chem. 273, 27492-27498
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Identification of 5'-deoxyribose phosphate lyase activity in human DNA polymerase gamma  and its role in mitochondrial base excision repair in vitro.
M. J. Longley, R. Prasad, D. K. Srivastava, S. H. Wilson, and W. C. Copeland (1998)
PNAS 95, 12244-12248
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Fidelity and Mutational Specificity of Uracil-initiated Base Excision DNA Repair Synthesis in Human Glioblastoma Cell Extracts.
R. J. S