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Science 28 July 1995: Vol. 269. no. 5223, pp. 496 - 512 DOI: 10.1126/science.7542800
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Articles
Science, Vol 269, Issue 5223, 496-512
Copyright © 1995 by American Association for the Advancement of Science
Whole-genome random sequencing and assembly of Haemophilus influenzae Rd
RD Fleischmann,
MD Adams,
O White,
RA Clayton,
EF Kirkness,
AR Kerlavage,
CJ Bult,
JF Tomb,
BA Dougherty,
JM Merrick,
and
al. et
Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830,137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.
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- Pyrroloquinoline Quinone-Dependent Dehydrogenases from Ketogulonicigenium vulgare Catalyze the Direct Conversion of L-Sorbosone to L-Ascorbic Acid.
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- Genome Plasticity and ori-ter Rebalancing in Salmonella typhi.
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- Putative ABC Transporter Responsible for Acetic Acid Resistance in Acetobacter aceti.
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- Exploration and Grading of Possible Genes from 183 Bacterial Strains by a Common Protocol to Identification of New Genes: Gene Trek in Prokaryote Space (GTPS).
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