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Science 28 July 1995:
Vol. 269. no. 5223, pp. 496 - 512
DOI: 10.1126/science.7542800

Articles

Science, Vol 269, Issue 5223, 496-512
Copyright © 1995 by American Association for the Advancement of Science


articles

Whole-genome random sequencing and assembly of Haemophilus influenzae Rd

RD Fleischmann, MD Adams, O White, RA Clayton, EF Kirkness, AR Kerlavage, CJ Bult, JF Tomb, BA Dougherty, JM Merrick, and al. et

Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830,137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.


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Haemophilus influenzae phasevarions have evolved from type III DNA restriction systems into epigenetic regulators of gene expression.
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Nucleic Acids Res.
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S. Nakagawa, Y. Takaki, S. Shimamura, A.-L. Reysenbach, K. Takai, and K. Horikoshi (2007)
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P. C. Juliao, C. F. Marrs, J. Xie, and J. R. Gilsdorf (2007)
J. Bacteriol. 189, 4994-5001
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VapC-1 of Nontypeable Haemophilus influenzae Is a Ribonuclease.
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J. Bacteriol. 189, 5041-5048
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Serial Analysis of rRNA Genes and the Unexpected Dominance of Rare Members of Microbial Communities.
M. N. Ashby, J. Rine, E. F. Mongodin, K. E. Nelson, and D. Dimster-Denk (2007)
Appl. Envir. Microbiol. 73, 4532-4542
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Ampicillin-Resistant Non-{beta}-Lactamase-Producing Haemophilus influenzae in Spain: Recent Emergence of Clonal Isolates with Increased Resistance to Cefotaxime and Cefixime.
S. Garcia-Cobos, J. Campos, E. Lazaro, F. Roman, E. Cercenado, C. Garcia-Rey, M. Perez-Vazquez, J. Oteo, and F. de Abajo (2007)
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What is a gene, post-ENCODE? History and updated definition.
M. B. Gerstein, C. Bruce, J. S. Rozowsky, D. Zheng, J. Du, J. O. Korbel, O. Emanuelsson, Z. D. Zhang, S. Weissman, and M. Snyder (2007)
Genome Res. 17, 669-681
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Diversity of antibiotic resistance integrative and conjugative elements among haemophili.
I. D. Dimopoulou, S. I. Kartali, R. M. Harding, T. E. A. Peto, and D. W. Crook (2007)
J. Med. Microbiol. 56, 838-846
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Genome of Methylobacillus flagellatus, Molecular Basis for Obligate Methylotrophy, and Polyphyletic Origin of Methylotrophy.
L. Chistoserdova, A. Lapidus, C. Han, L. Goodwin, L. Saunders, T. Brettin, R. Tapia, P. Gilna, S. Lucas, P. M. Richardson, et al. (2007)
J. Bacteriol. 189, 4020-4027
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Bacterial Transcytosis across Conjunctival M Cells.
C. K. Petris, M. Golomb, and T. E. Phillips (2007)
Invest. Ophthalmol. Vis. Sci. 48, 2172-2177
   Abstract »    Full Text »    PDF »
Advanced sequencing technologies and their wider impact in microbiology.
N. Hall (2007)
J. Exp. Biol. 210, 1518-1525
   Abstract »    Full Text »    PDF »
Mosaic Structure of p1658/97, a 125-Kilobase Plasmid Harboring an Active Amplicon with the Extended-Spectrum {beta}-Lactamase Gene blaSHV-5.
M. Zienkiewicz, I. Kern-Zdanowicz, M. Golebiewski, J. Zylinska, P. Mieczkowski, M. Gniadkowski, J. Bardowski, and P. Ceglowski (2007)
Antimicrob. Agents Chemother. 51, 1164-1171
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Precise Excision and Self-Integration of a Composite Transposon as a Model for Spontaneous Large-Scale Chromosome Inversion/Deletion of the Staphylococcus haemolyticus Clinical Strain JCSC1435.
S. Watanabe, T. Ito, Y. Morimoto, F. Takeuchi, and K. Hiramatsu (2007)
J. Bacteriol. 189, 2921-2925
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Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir.
L. Feng, W. Wang, J. Cheng, Y. Ren, G. Zhao, C. Gao, Y. Tang, X. Liu, W. Han, X. Peng, et al. (2007)
PNAS 104, 5602-5607
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DNA Sequencing: Strategies for Soil Microbiology.
M. D. Zwolinski (2007)
Soil Sci. Soc. Am. J. 71, 592-600
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Complete Genome Sequence of Haemophilus somnus (Histophilus somni) Strain 129Pt and Comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd.
J. F. Challacombe, A. J. Duncan, T. S. Brettin, D. Bruce, O. Chertkov, J. C. Detter, C. S. Han, M. Misra, P. Richardson, R. Tapia, et al. (2007)
J. Bacteriol. 189, 1890-1898
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Reduced Susceptibility of Haemophilus influenzae to the Peptide Deformylase Inhibitor LBM415 Can Result from Target Protein Overexpression Due to Amplified Chromosomal def Gene Copy Number.
C. R. Dean, S. Narayan, J. Richards, D. M. Daigle, S. Esterow, J. A. Leeds, H. Kamp, X. Puyang, B. Wiedmann, D. Mueller, et al. (2007)
Antimicrob. Agents Chemother. 51, 1004-1010
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Assembling millions of short DNA sequences using SSAKE.
R. L. Warren, G. G. Sutton, S. J. M. Jones, and R. A. Holt (2007)
Bioinformatics 23, 500-501
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Novel Type IV Secretion System Involved in Propagation of Genomic Islands.
M. Juhas, D. W. Crook, I. D. Dimopoulou, G. Lunter, R. M. Harding, D. J. P. Ferguson, and D. W. Hood (2007)
J. Bacteriol. 189, 761-771
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An Inward-Facing Conformation of a Putative Metal-Chelate-Type ABC Transporter.
H. W. Pinkett, A. T. Lee, P. Lum, K. P. Locher, and D. C. Rees (2007)
Science 315, 373-377
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Identification of the Functional Initiation Codons of a Phase-Variable Gene of Haemophilus influenzae, lic2A, with the Potential for Differential Expression.
K. Dixon, C. D. Bayliss, K. Makepeace, E. R. Moxon, and D. W. Hood (2007)
J. Bacteriol. 189, 511-521
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The haem-haemopexin utilization gene cluster (hxuCBA) as a virulence factor of Haemophilus influenzae.
D. J. Morton, T. W. Seale, L. L. Madore, T. M. VanWagoner, P. W. Whitby, and T. L. Stull (2007)
Microbiology 153, 215-224
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Identification of Genes Involved in the Expression of Atypical Lipooligosaccharide Structures from a Second Class of Haemophilus ducreyi.
D. M. B. Post, R. S. Munson Jr., B. Baker, H. Zhong, J. A. Bozue, and B. W. Gibson (2007)
Infect. Immun. 75, 113-121
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Identification of New Genetic Regions More Prevalent in Nontypeable Haemophilus influenzae Otitis Media Strains than in Throat Strains.
J. Xie, P. C. Juliao, J. R. Gilsdorf, D. Ghosh, M. Patel, and C. F. Marrs (2006)
J. Clin. Microbiol. 44, 4316-4325
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A Conserved Tetranucleotide Repeat Is Necessary for Wild-Type Expression of the Moraxella catarrhalis UspA2 Protein.
A. S. Attia and E. J. Hansen (2006)
J. Bacteriol. 188, 7840-7852
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Role of lgtC in Resistance of Nontypeable Haemophilus influenzae Strain R2866 to Human Serum.
A. L. Erwin, S. Allen, D. K. Ho, P. J. Bonthius, J. Jarisch, K. L. Nelson, D. L. Tsao, W. C. T. Unrath, M. E. Watson Jr., B. W. Gibson, et al. (2006)
Infect. Immun. 74, 6226-6235
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Presence of Copper- and Zinc-Containing Superoxide Dismutase in Commensal Haemophilus haemolyticus Isolates Can Be Used as a Marker To Discriminate Them from Nontypeable H. influenzae Isolates.
W. W. M. Fung, C. A. O'Dwyer, S. Sinha, A. L. Brauer, T. F. Murphy, J. S. Kroll, and P. R. Langford (2006)
J. Clin. Microbiol. 44, 4222-4226
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Specific Amino Acids of the Glycosyltransferase LpsA Direct the Addition of Glucose or Galactose to the Terminal Inner Core Heptose of Haemophilus influenzae Lipopolysaccharide via Alternative Linkages.
M. E. Deadman, S. L. Lundstrom, E. K. H. Schweda, E. R. Moxon, and D. W. Hood (2006)
J. Biol. Chem. 281, 29455-29467
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Engineered vaginal lactobacillus strain for mucosal delivery of the human immunodeficiency virus inhibitor cyanovirin-N..
X. Liu, L. A. Lagenaur, D. A. Simpson, K. P. Essenmacher, C. L. Frazier-Parker, Y. Liu, D. Tsai, S. S. Rao, D. H. Hamer, T. P. Parks, et al. (2006)
Antimicrob. Agents Chemother. 50, 3250-3259
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M. M. Fernaays, A. J. Lesse, X. Cai, and T. F. Murphy (2006)
Infect. Immun. 74, 5860-5870
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NAD+ utilization in pasteurellaceae: simplification of a complex pathway..
G. Gerlach and J. Reidl (2006)
J. Bacteriol. 188, 6719-6727
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Complete Genome Sequence of the Marine, Chemolithoautotrophic, Ammonia-Oxidizing Bacterium Nitrosococcus oceani ATCC 19707.
M. G. Klotz, D. J. Arp, P. S. G. Chain, A. F. El-Sheikh, L. J. Hauser, N. G. Hommes, F. W. Larimer, S. A. Malfatti, J. M. Norton, A. T. Poret-Peterson, et al. (2006)
Appl. Envir. Microbiol. 72, 6299-6315
   Abstract »    Full Text »    PDF »
Prediction of whole-genome DNA-DNA similarity, determination of G+C content and phylogenetic analysis within the family Pasteurellaceae by multilocus sequence analysis (MLSA)..
P. Kuhnert and B. M. Korczak (2006)
Microbiology 152, 2537-2548
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Molecular Evolution of {beta}-Lactam-Resistant Haemophilus influenzae: 9-Year Surveillance of Penicillin-Binding Protein 3 Mutations in Isolates from Japan..
Y. Sanbongi, T. Suzuki, Y. Osaki, N. Senju, T. Ida, and K. Ubukata (2006)
Antimicrob. Agents Chemother. 50, 2487-2492
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Differential Genome Contents of Nontypeable Haemophilus influenzae Strains from Adults with Chronic Obstructive Pulmonary Disease..
M. M. Fernaays, A. J. Lesse, S. Sethi, X. Cai, and T. F. Murphy (2006)
Infect. Immun. 74, 3366-3374
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Heterogeneity in Tandem Octanucleotides within Haemophilus influenzae Lipopolysaccharide Biosynthetic Gene losA Affects Serum Resistance..
A. L. Erwin, P. J. Bonthuis, J. L. Geelhood, K. L. Nelson, K. W. McCrea, J. R. Gilsdorf, and A. L. Smith (2006)
Infect. Immun. 74, 3408-3414
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The Haemophilus influenzae Type b hcsA and hcsB Gene Products Facilitate Transport of Capsular Polysaccharide across the Outer Membrane and Are Essential for Virulence.
S. Sukupolvi-Petty, S. Grass, and J. W. StGeme III (2006)
J. Bacteriol. 188, 3870-3877
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Comparative Genomics of NAD Biosynthesis in Cyanobacteria..
S. Y. Gerdes, O. V. Kurnasov, K. Shatalin, B. Polanuyer, R. Sloutsky, V. Vonstein, R. Overbeek, and A. L. Osterman (2006)
J. Bacteriol. 188, 3012-3023
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Microbial Phylogeny and Evolution: Concepts and Controversies.
J. O. Andersson (2006)
Syst Biol 55, 359-361
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Complete Genome Sequence of the Dehalorespiring Bacterium Desulfitobacterium hafniense Y51 and Comparison with Dehalococcoides ethenogenes 195.
H. Nonaka, G. Keresztes, Y. Shinoda, Y. Ikenaga, M. Abe, K. Naito, K. Inatomi, K. Furukawa, M. Inui, and H. Yukawa (2006)
J. Bacteriol. 188, 2262-2274
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Role of the tryptophan residue in the vicinity of the catalytic center of exonuclease III family AP endonucleases: AP site recognition mechanism.
K. Kaneda, J. Sekiguchi, and T. Shida (2006)
Nucleic Acids Res. 34, 1552-1563
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Genome Sequence of the Chemolithoautotrophic Nitrite-Oxidizing Bacterium Nitrobacter winogradskyi Nb-255..
S. R. Starkenburg, P. S. G. Chain, L. A. Sayavedra-Soto, L. Hauser, M. L. Land, F. W. Larimer, S. A. Malfatti, M. G. Klotz, P. J. Bottomley, D. J. Arp, et al. (2006)
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T. Miyazaki, T. Sugisawa, and T. Hoshino (2006)
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Genome Plasticity and ori-ter Rebalancing in Salmonella typhi.
G.-R. Liu, W.-Q. Liu, R. N. Johnston, K. E. Sanderson, S.-X. Li, and S.-L. Liu (2006)
Mol. Biol. Evol. 23, 365-371
   Abstract »    Full Text »    PDF »
Putative ABC Transporter Responsible for Acetic Acid Resistance in Acetobacter aceti.
S. Nakano, M. Fukaya, and S. Horinouchi (2006)
Appl. Envir. Microbiol. 72, 497-505
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Exploration and Grading of Possible Genes from 183 Bacterial Strains by a Common Protocol to Identification of New Genes: Gene Trek in Prokaryote Space (GTPS).
T. Kosuge, T. Abe, T. Okido, N. Tanaka, M. Hirahata, Y. Maruyama, J. Mashima, A. Tomiki, M. Kurokawa, R. Himeno, et al. (2006)
DNA Res 13, 245-254
   Abstract »    Full Text »    PDF »
Megx.net--database resources for marine ecological genomics.
T. Lombardot, R. Kottmann, H. Pfeffer, M. Richter, H. Teeling, C. Quast, and F. O. Glockner (2006)
Nucleic Acids Res. 34, D390-D393
   Abstract »    Full Text »    PDF »
Identification of oligonucleotide sequences that direct the movement of the Escherichia coli FtsK translocase.
O. Levy, J. L. Ptacin, P. J. Pease, J. Gore, M. B. Eisen, C. Bustamante, and N. R. Cozzarelli (2005)
PNAS 102, 17618-17623
   Abstract »    Full Text »    PDF »
Current trends in publicly available genetic databases.
M. G. Tyshenko and W. Leiss (2005)
Health Informatics Journal 11, 295-308
   Abstract »    PDF »
Insights on biology and evolution from microbial genome sequencing.
C. M. Fraser-Liggett (2005)
Genome Res. 15, 1603-1610
   Abstract »    Full Text »    PDF »



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