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Science 21 July 1995:
Vol. 269. no. 5222, pp. 396 - 400
DOI: 10.1126/science.7618104
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Articles

Science, Vol 269, Issue 5222, 396-400
Copyright © 1995 by American Association for the Advancement of Science

articles

Telomerase in yeast

M Cohn and EH Blackburn

Department of Microbiology and Immunology, University of California, San Francisco 94143-0414, USA.

The ribonucleoprotein enzyme telomerase synthesizes telomeric DNA by copying an internal RNA template sequence. The telomerase activities of the yeasts Saccharomyces castellii and Saccharomyces cerevisiae--with regular and irregular telomeric sequences, respectively--have now been identified and characterized. The S. cerevisiae activity required the telomerase RNA gene TLC1 but not the EST1 gene, both of which are required for normal telomere maintenance in vivo. This activity exhibited low processivity and produced no regularly repeated products. An inherently high stalling frequency of the S. cerevisiae telomerase may account for its in vitro properties and for the irregular telomeric sequences of this yeast.


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Functional Analysis of Conserved Residues in the Putative "Finger" Domain of Telomerase Reverse Transcriptase.
D. Bosoy and N. F. Lue (2001)
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Molecular Basis for Telomere Repeat Divergence in Budding Yeast.
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New Function of CDC13 in Positive Telomere Length Regulation.
B. Meier, L. Driller, S. Jaklin, and H. M. Feldmann (2001)
Mol. Cell. Biol. 21, 4233-4245
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Yeast telomerase appears to frequently copy the entire template in vivo.
A. Ray and K. W. Runge (2001)
Nucleic Acids Res. 29, 2382-2394
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Characterization of the Interaction between the Nuclease and Reverse Transcriptase Activity of the Yeast Telomerase Complex.
H. Niu, J. Xia, and N. F. Lue (2000)
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Identification of Functionally Important Domains in the N-Terminal Region of Telomerase Reverse Transcriptase.
J. Xia, Y. Peng, I. S. Mian, and N. F. Lue (2000)
Mol. Cell. Biol. 20, 5196-5207
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Saccharomyces cerevisiae RAP1 binds to telomeric sequences with spatial flexibility.
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Heterogeneous Nuclear Ribonucleoprotein A1 and UP1 Protect Mammalian Telomeric Repeats and Modulate Telomere Replication in Vitro.
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Telomerase RNA Template Mutations Reveal Sequence-Specific Requirements for the Activation and Repression of Telomerase Action at Telomeres.
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Positive and negative regulation of telomerase access to the telomere.
S. Evans and V Lundblad (2000)
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Functional Reconstitution of Human Telomerase Expressed in Saccharomyces cerevisiae.
F. Bachand and C. Autexier (1999)
J. Biol. Chem. 274, 38027-38031
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Essential functions of amino-terminal domains in the yeast telomerase catalytic subunit revealed by selection for viable mutants.
K. L. Friedman and T. R. Cech (1999)
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Telomeres and telomerase.
M. A. Blasco, S. M. Gasser, and J. Lingner (1999)
Genes & Dev. 13, 2353-2359
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Developmentally Regulated Telomerase Activity Is Correlated with Chromosomal Healing during Chromatin Diminution in Ascaris suum.
L. Magnenat, H. Tobler, and F. Muller (1999)
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   Abstract »    Full Text »    PDF »
Expression of Mutated Paramecium Telomerase RNAs In Vivo Leads to Templating Errors That Resemble Those Made by Retroviral Reverse Transcriptase.
A. J. Ye, W. J. Haynes, and D. P. Romero (1999)
Mol. Cell. Biol. 19, 2887-2894
   Abstract »    Full Text »    PDF »
Telomerase in kinetoplastid parasitic protozoa.
M. I. N. Cano, J. M. Dungan, N. Agabian, and E. H. Blackburn (1999)
PNAS 96, 3616-3621
   Abstract »    Full Text »    PDF »
Y'-Help1, a DNA Helicase Encoded by the Yeast Subtelomeric Y' Element, Is Induced in Survivors Defective for Telomerase.
M. Yamada, N. Hayatsu, A. Matsuura, and F. Ishikawa (1998)
J. Biol. Chem. 273, 33360-33366
   Abstract »    Full Text »    PDF »
Specific telomerase RNA residues distant from the template are essential for telomerase function.
J. Roy, T. B. Fulton, and E. H. Blackburn (1998)
Genes & Dev. 12, 3286-3300
   Abstract »    Full Text »
Identification of Kluyveromyces lactis Telomerase: Discontinuous Synthesis along the 30-Nucleotide-Long Templating Domain.
T. B. Fulton and E. H. Blackburn (1998)
Mol. Cell. Biol. 18, 4961-4970
   Abstract »    Full Text »
The reverse transcriptase component of the Tetrahymena telomerase ribonucleoprotein complex.
K. Collins and L. Gandhi (1998)
PNAS 95, 8485-8490
   Abstract »    Full Text »    PDF »
The telomerase reverse transcriptase: components and regulation.
C. I. Nugent and V. Lundblad (1998)
Genes & Dev. 12, 1073-1085
   Full Text »
The C Terminus of the Major Yeast Telomere Binding Protein Rap1p Enhances Telomere Formation.
A. Ray and K. W. Runge (1998)
Mol. Cell. Biol. 18, 1284-1295
   Abstract »    Full Text »
Interaction of recombinant Tetrahymena telomerase proteins p80 and p95 with telomerase RNA and telomeric DNA substrates.
L. Gandhi and K. Collins (1998)
Genes & Dev. 12, 721-733
   Abstract »    Full Text »
Functionally interacting telomerase RNAs in the yeast telomerase complex.
J. Prescott and E. H. Blackburn (1997)
Genes & Dev. 11, 2790-2800
   Abstract »    Full Text »    PDF »
Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity.
J. Lingner, T. R. Cech, T. R. Hughes, and V. Lundblad (1997)
PNAS 94, 11190-11195
   Abstract »    Full Text »    PDF »
The catalytic subunit of yeast telomerase.
C. M. Counter, M. Meyerson, E. N. Eaton, and R. A. Weinberg (1997)
PNAS 94, 9202-9207
   Abstract »    Full Text »    PDF »
A novel Rap1p-interacting factor, Rif2p, cooperates with Rif1p to regulate telomere length in Saccharomyces cerevisiae..
D Wotton and D Shore (1997)
Genes & Dev. 11, 748-760
   Abstract »    PDF »
Telomerase RNA mutations in Saccharomyces cerevisiae alter telomerase action and reveal nonprocessivity in vivo and in vitro..
J Prescott and E H Blackburn (1997)
Genes & Dev. 11, 528-540
   Abstract »    PDF »
Est1 has the properties of a single-stranded telomere end-binding protein..
V Virta-Pearlman, D K Morris, and V Lundblad (1996)
Genes & Dev. 10, 3094-3104
   Abstract »    PDF »
Characterization and developmental patterns of telomerase expression in plants.
M. S. Fitzgerald, T. D. McKnight, and D. E. Shippen (1996)
PNAS 93, 14422-14427
   Abstract »    Full Text »    PDF »
Cell cycle-regulated generation of single-stranded G-rich DNA in the absence of telomerase.
I. Dionne and R. J. Wellinger (1996)
PNAS 93, 13902-13907
   Abstract »    Full Text »    PDF »
Cap-prevented recombination between terminal telomeric repeat arrays (telomere CPR) maintains telomeres in Kluyveromyces lactis lacking telomerase..
M J McEachern and E H Blackburn (1996)
Genes & Dev. 10, 1822-1834
   Abstract »    PDF »
A novel mechanism for telomere size control in Saccharomyces cerevisiae..
B Li and A J Lustig (1996)
Genes & Dev. 10, 1310-1326
   Abstract »    PDF »
Telomeres: Beginning to Understand the End.
V. A. Zakian (1995)
Science 270, 1601-1607
   Abstract »    PDF »
Altering specific telomerase RNA template residues affects active site function..
D Gilley, M S Lee, and E H Blackburn (1995)
Genes & Dev. 9, 2214-2226
   Abstract »    PDF »
Boundary elements of the Tetrahymena telomerase RNA template and alignment domains..
C Autexier and C W Greider (1995)
Genes & Dev. 9, 2227-2239
   Abstract »    PDF »


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