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Science 14 July 1995:
Vol. 269. no. 5221, pp. 230 - 234
DOI: 10.1126/science.7618084

Articles

Science, Vol 269, Issue 5221, 230-234
Copyright © 1995 by American Association for the Advancement of Science


articles

Targeted disruption of mouse EGF receptor: effect of genetic background on mutant phenotype

DW Threadgill, AA Dlugosz, LA Hansen, T Tennenbaum, U Lichti, D Yee, C LaMantia, T Mourton, K Herrup, RC Harris, and al. et

Department of Genetics, Case Western Reserve University, Cleveland, OH 44106-4955, USA.

Gene targeting was used to create a null allele at the epidermal growth factor receptor locus (Egfr). The phenotype was dependent on genetic background. EGFR deficiency on a CF-1 background resulted in peri-implantation death due to degeneration of the inner cell mass. On a 129/Sv background, homozygous mutants died at mid-gestation due to placental defects; on a CD-1 background, the mutants lived for up to 3 weeks and showed abnormalities in skin, kidney, brain, liver, and gastrointestinal tract. The multiple abnormalities associated with EGFR deficiency indicate that the receptor is involved in a wide range of cellular activities.


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Cloning of porcine signal transducer and activator of transcription 3 cDNA and its expression in reproductive tissues..
L. Wen, J. Craig, P. W Dyce, and J. Li (2006)
Reproduction 132, 511-518
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Epidermal Growth Factor Receptor Is a Critical Mediator of Ultraviolet B Irradiation-Induced Signal Transduction in Immortalized Human Keratinocyte HaCaT Cells.
Y. Xu, J. J. Voorhees, and G. J. Fisher (2006)
Am. J. Pathol. 169, 823-830
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Selective Modulation of Hedgehog/GLI Target Gene Expression by Epidermal Growth Factor Signaling in Human Keratinocytes..
M. Kasper, H. Schnidar, G. W. Neill, M. Hanneder, S. Klingler, L. Blaas, C. Schmid, C. Hauser-Kronberger, G. Regl, M. P. Philpott, et al. (2006)
Mol. Cell. Biol. 26, 6283-6298
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Strategies to enhance epidermal growth factor inhibition: targeting the mevalonate pathway..
J. Dimitroulakos, I. A. Lorimer, and G. Goss (2006)
Clin. Cancer Res. 12, 4426s-4431s
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Prediction of heterogeneity in intelligence and adult prognosis by genetic polymorphisms in the dopamine system among children with attention-deficit/hyperactivity disorder: evidence from 2 birth cohorts..
J. Mill, A. Caspi, B. S. Williams, I. Craig, A. Taylor, M. Polo-Tomas, C. W. Berridge, R. Poulton, and T. E. Moffitt (2006)
Arch Gen Psychiatry 63, 462-469
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Angiotensin II Type 1 Receptor-EGF Receptor Cross-Talk Regulates Ureteric Bud Branching Morphogenesis.
I. V. Yosypiv, M. Schroeder, and S. S. El-Dahr (2006)
J. Am. Soc. Nephrol. 17, 1005-1014
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Ultraviolet irradiation induces keratinocyte proliferation and epidermal hyperplasia through the activation of the epidermal growth factor receptor.
T. B. El-Abaseri, S. Putta, and L. A. Hansen (2006)
Carcinogenesis 27, 225-231
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AP-2{alpha}: a regulator of EGF receptor signaling and proliferation in skin epidermis.
X. Wang, D. Bolotin, D. H. Chu, L. Polak, T. Williams, and E. Fuchs (2006)
J. Cell Biol. 172, 409-421
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Blockade of EGFR and ErbB2 by the Novel Dual EGFR and ErbB2 Tyrosine Kinase Inhibitor GW572016 Sensitizes Human Colon Carcinoma GEO Cells to Apoptosis.
Y. Zhou, S. Li, Y. P. Hu, J. Wang, J. Hauser, A. N. Conway, M. A. Vinci, L. Humphrey, E. Zborowska, J. K.V. Willson, et al. (2006)
Cancer Res. 66, 404-411
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Patch-clamp study reveals that the importance of connexin43-mediated gap junctional communication for ovarian folliculogenesis is strain specific in the mouse.
D. Tong, J. E. I. Gittens, G. M. Kidder, and D. Bai (2006)
Am J Physiol Cell Physiol 290, C290-C297
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HB-EGF promotes epithelial cell migration in eyelid development.
N. Mine, R. Iwamoto, and E. Mekada (2005)
Development 132, 4317-4326
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PKD2 Functions as an Epidermal Growth Factor-Activated Plasma Membrane Channel.
R. Ma, W.-P. Li, D. Rundle, J. Kong, H. I. Akbarali, and L. Tsiokas (2005)
Mol. Cell. Biol. 25, 8285-8298
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Clinical signs, pathophysiology and management of skin toxicity during therapy with epidermal growth factor receptor inhibitors.
S. Segaert and E. Van Cutsem (2005)
Ann. Onc. 16, 1425-1433
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Mammary ductal morphogenesis requires paracrine activation of stromal EGFR via ADAM17-dependent shedding of epithelial amphiregulin.
M. D. Sternlicht, S. W. Sunnarborg, H. Kouros-Mehr, Y. Yu, D. C. Lee, and Z. Werb (2005)
Development 132, 3923-3933
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Strain-Dependent Influences on the Hypothalamo-Pituitary-Adrenal Axis Profoundly Affect the 7B2 and PC2 Null Phenotypes.
J. R. Peinado, V. Laurent, S.-N. Lee, B. W. Peng, J. E. Pintar, D. F. Steiner, and I. Lindberg (2005)
Endocrinology 146, 3438-3444
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ROCK-I and ROCK-II cooperatively regulate closure of eyelid and ventral body wall in mouse embryo.
D. Thumkeo, Y. Shimizu, S. Sakamoto, S. Yamada, and S. Narumiya (2005)
Genes Cells 10, 825-834
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A dual role of FGF10 in proliferation and coordinated migration of epithelial leading edge cells during mouse eyelid development.
H. Tao, M. Shimizu, R. Kusumoto, K. Ono, S. Noji, and H. Ohuchi (2005)
Development 132, 3217-3230
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A Structural Model for the Membrane-bound Form of the Juxtamembrane Domain of the Epidermal Growth Factor Receptor.
K. Choowongkomon, C. R. Carlin, and F. D. Sonnichsen (2005)
J. Biol. Chem. 280, 24043-24052
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p38 Kinase-mediated Transactivation of the Epidermal Growth Factor Receptor Is Required for Dedifferentiation of Renal Epithelial Cells after Oxidant Injury.
S. Zhuang, Y. Yan, J. Han, and R. G. Schnellmann (2005)
J. Biol. Chem. 280, 21036-21042
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Development of Structures and Transport Functions in the Mouse Placenta.
E. D. Watson and J. C. Cross (2005)
Physiology 20, 180-193
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Stress-induced variation in evolution: from behavioural plasticity to genetic assimilation.
A. V Badyaev (2005)
Proc R Soc B 272, 877-886
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Chemoprevention of UV Light-Induced Skin Tumorigenesis by Inhibition of the Epidermal Growth Factor Receptor.
T. B. El-Abaseri, J. Fuhrman, C. Trempus, I. Shendrik, R. W. Tennant, and L. A. Hansen (2005)
Cancer Res. 65, 3958-3965
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Dynamic Assembly of the Urokinase-type Plasminogen Activator Signaling Receptor Complex Determines the Mitogenic Activity of Urokinase-type Plasminogen Activator.
M. Jo, K. S. Thomas, N. Marozkina, T. J. Amin, C. M. Silva, S. J. Parsons, and S. L. Gonias (2005)
J. Biol. Chem. 280, 17449-17457
   Abstract »    Full Text »    PDF »
Involvement of a Rac Activator, P-Rex1, in Neurotrophin-Derived Signaling and Neuronal Migration.
M. Yoshizawa, T. Kawauchi, M. Sone, Y. V. Nishimura, M. Terao, K. Chihama, Y.-i. Nabeshima, and M. Hoshino (2005)
J. Neurosci. 25, 4406-4419
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Inhibition of the epidermal growth factor receptor increases expression of genes that stimulate inflammation, apoptosis, and cell attachment.
C. D. Woodworth, E. Michael, D. Marker, S. Allen, L. Smith, and M. Nees (2005)
Mol. Cancer Ther. 4, 650-658
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ROCK-I regulates closure of the eyelids and ventral body wall by inducing assembly of actomyosin bundles.
Y. Shimizu, D. Thumkeo, J. Keel, T. Ishizaki, H. Oshima, M. Oshima, Y. Noda, F. Matsumura, M. M. Taketo, and S. Narumiya (2005)
J. Cell Biol. 168, 941-953
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Modeling metastasis in vivo.
C. Khanna and K. Hunter (2005)
Carcinogenesis 26, 513-523
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Combined Targeting of the Estrogen Receptor and the Epidermal Growth Factor Receptor in Non-Small Cell Lung Cancer Shows Enhanced Antiproliferative Effects.
L. P. Stabile, J. S. Lyker, C. T. Gubish, W. Zhang, J. R. Grandis, and J. M. Siegfried (2005)
Cancer Res. 65, 1459-1470
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Gefitinib ("Iressa", ZD1839), an Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor, Reverses Breast Cancer Resistance Protein/ABCG2-Mediated Drug Resistance.
Y. Nakamura, M. Oka, H. Soda, K. Shiozawa, M. Yoshikawa, A. Itoh, Y. Ikegami, J. Tsurutani, K. Nakatomi, T. Kitazaki, et al. (2005)
Cancer Res. 65, 1541-1546
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Myelinogenesis and Axonal Recognition by Oligodendrocytes in Brain Are Uncoupled in Olig1-Null Mice.
M. Xin, T. Yue, Z. Ma, F.-f. Wu, A. Gow, and Q. R. Lu (2005)
J. Neurosci. 25, 1354-1365
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Amphiregulin Is a Novel Growth Factor Involved in Normal Bone Development and in the Cellular Response to Parathyroid Hormone Stimulation.
L. Qin, J. Tamasi, L. Raggatt, X. Li, J. H. M. Feyen, D. C. Lee, E. DiCicco-Bloom, and N. C. Partridge (2005)
J. Biol. Chem. 280, 3974-3981
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ADAM10 Mediates Ectodomain Shedding of the Betacellulin Precursor Activated by p-Aminophenylmercuric Acetate and Extracellular Calcium Influx.
M. P. Sanderson, S. N. Erickson, P. J. Gough, K. J. Garton, P. T. Wille, E. W. Raines, A. J. Dunbar, and P. J. Dempsey (2005)
J. Biol. Chem. 280, 1826-1837
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Epidermal Growth Factor Receptor-deficient Mice Have Delayed Primary Endochondral Ossification Because of Defective Osteoclast Recruitment.
K. Wang, H. Yamamoto, J. R. Chin, Z. Werb, and T. H. Vu (2004)
J. Biol. Chem. 279, 53848-53856
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Multiple urinary tract malformations with likely recessive inheritance in a large Somalian kindred.
A. Pasch, J. Hoefele, H. Grimminger, H.-W. Hacker, and F. Hildebrandt (2004)
Nephrol. Dial. Transplant. 19, 3172-3175
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Embryo-Uterine Interactions via the Neuregulin Family of Growth Factors During Implantation in the Mouse.
N. Brown, K. Deb, B. C. Paria, S. K. Das, and J. Reese (2004)
Biol Reprod 71, 2003-2011
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Requirement of the forkhead gene Foxe1, a target of sonic hedgehog signaling, in hair follicle morphogenesis.
A. Brancaccio, A. Minichiello, M. Grachtchouk, D. Antonini, H. Sheng, R. Parlato, N. Dathan, A. A. Dlugosz, and C. Missero (2004)
Hum. Mol. Genet. 13, 2595-2606
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Epidermal Growth Factor-stimulated Intestinal Epithelial Cell Migration Requires Src Family Kinase-dependent p38 MAPK Signaling.
M. R. Frey, A. Golovin, and D. B. Polk (2004)
J. Biol. Chem. 279, 44513-44521
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Epiregulin Is Not Essential for Development of Intestinal Tumors but Is Required for Protection from Intestinal Damage.
D. Lee, R. S. Pearsall, S. Das, S. K. Dey, V. L. Godfrey, and D. W. Threadgill (2004)
Mol. Cell. Biol. 24, 8907-8916
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Targeted Disruption of the Protein Kinase SGK3/CISK Impairs Postnatal Hair Follicle Development.
J. A. McCormick, Y. Feng, K. Dawson, M. J. Behne, B. Yu, J. Wang, A. W. Wyatt, G. Henke, F. Grahammer, T. M. Mauro, et al. (2004)
Mol. Biol. Cell 15, 4278-4288
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Mechanisms and Limits of Induced Postnatal Lung Growth.
(2004)
Am. J. Respir. Crit. Care Med. 170, 319-343
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Phenotypic Variation Resulting From a Deficiency of Epidermal Growth Factor Receptor in Mice Is Caused by Extensive Genetic Heterogeneity That Can Be Genetically and Molecularly Partitioned.
K. E. Strunk, V. Amann, and D. W. Threadgill (2004)
Genetics 167, 1821-1832
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Development of the Novel Biologically Targeted Anticancer Agent Gefitinib: Determining the Optimum Dose for Clinical Efficacy.
M. Wolf, H. Swaisland, and S. Averbuch (2004)
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Selective Roles for Tumor Necrosis Factor {alpha}-converting Enzyme/ADAM17 in the Shedding of the Epidermal Growth Factor Receptor Ligand Family: THE JUXTAMEMBRANE STALK DETERMINES CLEAVAGE EFFICIENCY.
C. L. Hinkle, S. W. Sunnarborg, D. Loiselle, C. E. Parker, M. Stevenson, W. E. Russell, and D. C. Lee (2004)
J. Biol. Chem. 279, 24179-24188
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Molecular Cues to Implantation.
S. K. Dey, H. Lim, S. K. Das, J. Reese, B. C. Paria, T. Daikoku, and H. Wang (2004)
Endocr. Rev. 25, 341-373
   Abstract »    Full Text »    PDF »
Activating Mutations in the Epidermal Growth Factor Receptor Underlying Responsiveness of Non-Small-Cell Lung Cancer to Gefitinib.
T. J. Lynch, D. W. Bell, R. Sordella, S. Gurubhagavatula, R. A. Okimoto, B. W. Brannigan, P. L. Harris, S. M. Haserlat, J. G. Supko, F. G. Haluska, et al. (2004)
N. Engl. J. Med. 350, 2129-2139
   Abstract »    Full Text »    PDF »
Electroporation of Murine Embryonic Stem Cells: A Step-by-Step Guide.
D. M. Tompers and P. A. Labosky (2004)
Stem Cells 22, 243-249
   Abstract »    Full Text »    PDF »
Branching morphogenesis and kidney disease.
M. M. Shah, R. V. Sampogna, H. Sakurai, K. T. Bush, and S. K. Nigam (2004)
Development 131, 1449-1462
   Abstract »    Full Text »    PDF »
Rescue of Female Infertility from the Loss of Cyclooxygenase-2 by Compensatory Up-regulation of Cyclooxygenase-1 Is a Function of Genetic Makeup.
H. Wang, W.-g. Ma, L. Tejada, H. Zhang, J. D. Morrow, S. K. Das, and S. K. Dey (2004)
J. Biol. Chem. 279, 10649-10658
   Abstract »    Full Text »    PDF »
Centromere protein b-null mice display decreasing reproductive performance through successive generations of breeding due to diminishing endometrial glands.
K J Fowler, L H Wong, B K Griffiths, M C Sibson, S Reed, and K H A Choo (2004)
Reproduction 127, 367-377
   Abstract »    Full Text »    PDF »
Distinct roles for ADAM10 and ADAM17 in ectodomain shedding of six EGFR ligands.
U. Sahin, G. Weskamp, K. Kelly, H.-M. Zhou, S. Higashiyama, J. Peschon, D. Hartmann, P. Saftig, and C. P. Blobel (2004)
J. Cell Biol. 164, 769-779
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Identification of a Second egfr Gene in Xiphophorus Uncovers an Expansion of the Epidermal Growth Factor Receptor Family in Fish.
A. Gomez, J.-N. Volff, U. Hornung, M. Schartl, and C. Wellbrock (2004)
Mol. Biol. Evol. 21, 266-275
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Epidermal Growth Factor Receptor Activation Differentially Regulates Claudin Expression and Enhances Transepithelial Resistance in Madin-Darby Canine Kidney Cells.
A. B. Singh and R. C. Harris (2004)
J. Biol. Chem. 279, 3543-3552
   Abstract »    Full Text »    PDF »
Velvet, a Dominant Egfr Mutation That Causes Wavy Hair and Defective Eyelid Development in Mice.
X. Du, K. Tabeta, K. Hoebe, H. Liu, N. Mann, S. Mudd, K. Crozat, S. Sovath, X. Gong, and B. Beutler (2004)
Genetics 166, 331-340
   Abstract »    Full Text »    PDF »
Moving forward with chemical mutagenesis in the mouse.
T. P. O'Brien and W. N. Frankel (2004)
J. Physiol. 554, 13-21
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Application of chromosomal substitution techniques in gene-function discovery.
A. W. Cowley Jr, R. J. Roman, and H. J. Jacob (2004)
J. Physiol. 554, 46-55
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Renal expression and activity of the germinal center kinase SK2.
A. V. Cybulsky, T. Takano, J. Papillon, A. Khadir, K. Bijian, C.-C. Chien, C. E. Alpers, and H. Rabb (2004)
Am J Physiol Renal Physiol 286, F16-F25
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Importance of Functional EGF Receptors in Recovery from Acute Nephrotoxic Injury.
Z. Wang, J.-K. Chen, S.-w. Wang, G. Moeckel, and R. C. Harris (2003)
J. Am. Soc. Nephrol. 14, 3147-3154
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Small-Molecule Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors.
M. L. Janmaat and G. Giaccone (2003)
Oncologist 8, 576-586
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Cbl-3-Deficient Mice Exhibit Normal Epithelial Development.
E. K. Griffiths, O. Sanchez, P. Mill, C. Krawczyk, C. V. Hojilla, E. Rubin, M. M. Nau, R. Khokha, S. Lipkowitz, C.-c. Hui, et al. (2003)
Mol. Cell. Biol. 23, 7708-7718
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Compromised Reproductive Function in Adult Female Mice Selectively Expressing Mutant ErbB-1 Tyrosine Kinase Receptors in Astroglia.
B. Li, Z. Yang, J. Hou, A. McCracken, M. A. Jennings, and M. Y. J. Ma (2003)
Mol. Endocrinol. 17, 2365-2376
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Adrenomedullin Is Decreased in Preeclampsia Because of Failed Response to Epidermal Growth Factor and Impaired Syncytialization.
H. Li, J. Dakour, S. Kaufman, L. J. Guilbert, B. Winkler-Lowen, and D. W. Morrish (2003)
Hypertension 42, 895-900
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