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Science 23 June 1995:
Vol. 268. no. 5218, pp. 1745 - 1748
DOI: 10.1126/science.7792599
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Articles

Science, Vol 268, Issue 5218, 1745-1748
Copyright © 1995 by American Association for the Advancement of Science

articles

ILR1, an amidohydrolase that releases active indole-3-acetic acid from conjugates

B Bartel and GR Fink

Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142, USA.

In plants, the growth regulator indole-3-acetic acid (IAA) is found both free and conjugated to a variety of amino acids, peptides, and carbohydrates. IAA conjugated to leucine has effects in Arabidopsis thaliana similar to those of free IAA. The ilr1 mutant is insensitive to exogenous IAA-Leu and was used to positionally clone the Arabidopsis ILR1 gene. ILR1 encodes a 48-kilodalton protein that cleaves IAA-amino acid conjugates in vitro and is homologous to bacterial amidohydrolase enzymes. DNA sequences similar to that of ILR1 are found in other plant species.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identification and Characterization of Arabidopsis Indole-3-Butyric Acid Response Mutants Defective in Novel Peroxisomal Enzymes.
B. K. Zolman, N. Martinez, A. Millius, A. R. Adham, and B. Bartel (2008)
Genetics 180, 237-251
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Inactive Methyl Indole-3-Acetic Acid Ester Can Be Hydrolyzed and Activated by Several Esterases Belonging to the AtMES Esterase Family of Arabidopsis.
Y. Yang, R. Xu, C.-j. Ma, A. C. Vlot, D. F. Klessig, and E. Pichersky (2008)
Plant Physiology 147, 1034-1045
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Dual Regulation Role of GH3.5 in Salicylic Acid and Auxin Signaling during Arabidopsis-Pseudomonas syringae Interaction.
Z. Zhang, Q. Li, Z. Li, P. E. Staswick, M. Wang, Y. Zhu, and Z. He (2007)
Plant Physiology 145, 450-464
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MYC2 Differentially Modulates Diverse Jasmonate-Dependent Functions in Arabidopsis.
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PLANT CELL 19, 2225-2245
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Mutation of E1-CONJUGATING ENZYME-RELATED1 Decreases RELATED TO UBIQUITIN Conjugation and Alters Auxin Response and Development.
A. W. Woodward, S. E. Ratzel, E. E. Woodward, Y. Shamoo, and B. Bartel (2007)
Plant Physiology 144, 976-987
   Abstract »    Full Text »    PDF »
Resistance to Botrytis cinerea Induced in Arabidopsis by Elicitors Is Independent of Salicylic Acid, Ethylene, or Jasmonate Signaling But Requires PHYTOALEXIN DEFICIENT3.
S. Ferrari, R. Galletti, C. Denoux, G. De Lorenzo, F. M. Ausubel, and J. Dewdney (2007)
Plant Physiology 144, 367-379
   Abstract »    Full Text »    PDF »
An Arabidopsis Basic Helix-Loop-Helix Leucine Zipper Protein Modulates Metal Homeostasis and Auxin Conjugate Responsiveness.
R. A. Rampey, A. W. Woodward, B. N. Hobbs, M. P. Tierney, B. Lahner, D. E. Salt, and B. Bartel (2006)
Genetics 174, 1841-1857
   Abstract »    Full Text »    PDF »
Enzymatic activity of Campylobacter jejuni hippurate hydrolase.
M. Steele, M. Marcone, C. Gyles, V.L. Chan, and J. Odumeru (2006)
Protein Eng. Des. Sel. 19, 17-25
   Abstract »    Full Text »    PDF »
An Indole-3-Acetic Acid Carboxyl Methyltransferase Regulates Arabidopsis Leaf Development.
G. Qin, H. Gu, Y. Zhao, Z. Ma, G. Shi, Y. Yang, E. Pichersky, H. Chen, M. Liu, Z. Chen, et al. (2005)
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Auxin: Regulation, Action, and Interaction.
A. W. WOODWARD and B. BARTEL (2005)
Ann. Bot. 95, 707-735
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Characterization of an Arabidopsis Enzyme Family That Conjugates Amino Acids to Indole-3-Acetic Acid.
P. E. Staswick, B. Serban, M. Rowe, I. Tiryaki, M. T. Maldonado, M. C. Maldonado, and W. Suza (2005)
PLANT CELL 17, 616-627
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His-404 and His-405 are Essential for Enzyme Catalytic Activities of a Bacterial Indole-3-Acetyl-L-Aspartic Acid Hydrolase.
J.-C. Chou, W. H. Welch, and J. D. Cohen (2004)
Plant Cell Physiol. 45, 1335-1341
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A Novel Auxin Conjugate Hydrolase from Wheat with Substrate Specificity for Longer Side-Chain Auxin Amide Conjugates.
J. J. Campanella, A. F. Olajide, V. Magnus, and J. Ludwig-Muller (2004)
Plant Physiology 135, 2230-2240
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A Family of Auxin-Conjugate Hydrolases That Contributes to Free Indole-3-Acetic Acid Levels during Arabidopsis Germination.
R. A. Rampey, S. LeClere, M. Kowalczyk, K. Ljung, G. Sandberg, and B. Bartel (2004)
Plant Physiology 135, 978-988
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IAR4, a Gene Required for Auxin Conjugate Sensitivity in Arabidopsis, Encodes a Pyruvate Dehydrogenase E1{alpha} Homolog.
S. LeClere, R. A. Rampey, and B. Bartel (2004)
Plant Physiology 135, 989-999
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A Specific Bacterial Aminoacylase Cleaves Odorant Precursors Secreted in the Human Axilla.
A. Natsch, H. Gfeller, P. Gygax, J. Schmid, and G. Acuna (2003)
J. Biol. Chem. 278, 5718-5727
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Characterization of a Family of IAA-Amino Acid Conjugate Hydrolases from Arabidopsis.
S. LeClere, R. Tellez, R. A. Rampey, S. P. T. Matsuda, and B. Bartel (2002)
J. Biol. Chem. 277, 20446-20452
   Abstract »    Full Text »    PDF »
Quantitative Analysis of Indole-3-Acetic Acid Metabolites in Arabidopsis.
M. Kowalczyk and G. Sandberg (2001)
Plant Physiology 127, 1845-1853
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BIG: a calossin-like protein required for polar auxin transport in Arabidopsis.
P. Gil, E. Dewey, J. Friml, Y. Zhao, K. C. Snowden, J. Putterill, K. Palme, M. Estelle, and J. Chory (2001)
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Characterization of an Aminoacylase from the Hyperthermophilic Archaeon Pyrococcus furiosus.
S. V. Story, A. M. Grunden, and M. W. W. Adams (2001)
J. Bacteriol. 183, 4259-4268
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Carrier-Mediated Uptake and Phloem Systemy of a 350-Dalton Chlorinated Xenobiotic with an {alpha}-Amino Acid Function.
C. Delétage-Grandon, J.-F. Chollet, M. Faucher, F. Rocher, E. Komor, and J.-L. Bonnemain (2001)
Plant Physiology 125, 1620-1632
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A Gain-of-Function Mutation in IAA28 Suppresses Lateral Root Development.
L. E. Rogg, J. Lasswell, and B. Bartel (2001)
PLANT CELL 13, 465-480
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KOJAK encodes a cellulose synthase-like protein required for root hair cell morphogenesis in Arabidopsis.
B. Favery, E. Ryan, J. Foreman, P. Linstead, K. Boudonck, M. Steer, P. Shaw, and L. Dolan (2001)
Genes & Dev. 15, 79-89
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Cloning and Characterization of IAR1, a Gene Required for Auxin Conjugate Sensitivity in Arabidopsis.
J. Lasswell, L. E. Rogg, D. C. Nelson, C. Rongey, and B. Bartel (2000)
PLANT CELL 12, 2395-2408
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Characterization of Auxin Conjugates in Arabidopsis. Low Steady-State Levels of Indole-3-Acetyl-Aspartate, Indole-3-Acetyl-Glutamate, and Indole-3-Acetyl-Glucose.
Y. Y. Tam, E. Epstein, and J. Normanly (2000)
Plant Physiology 123, 589-596
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Auxin metabolism in mosses and liverworts.
A. Ester Sztein, J. D. Cohen, I. G. de la Fuente, and T. J. Cooke (1999)
Am. J. Botany 86, 1544-1555
   Abstract »    Full Text »    PDF »
IAR3 Encodes an Auxin Conjugate Hydrolase from Arabidopsis.
R. T. Davies, D. H. Goetz, J. Lasswell, M. N. Anderson, and B. Bartel (1999)
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Metabolism of Indole-3-Acetic Acid in Arabidopsis.
A. Östin, M. Kowalyczk, R. P. Bhalerao, and G. Sandberg (1998)
Plant Physiology 118, 285-296
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