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Science 16 June 1995:
Vol. 268. no. 5217, pp. 1616 - 1619
DOI: 10.1126/science.7777859
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Articles

Science, Vol 268, Issue 5217, 1616-1619
Copyright © 1995 by American Association for the Advancement of Science

articles

Association of increased spontaneous mutation rates with high levels of transcription in yeast

A Datta and S Jinks-Robertson

Department of Biology, Emory University, Atlanta, GA 30322, USA.

Complex processes such as transcription, replication, repair, and recombination require changes in chromatin structure and the interactions of numerous trans-acting factors with DNA sequences, raising the possibility that these processes may be interrelated. Here the effect of transcription on the rate of spontaneous mutation in the yeast Saccharomyces cerevisiae was examined. With the use of a lys2 frameshift allele under the control of a highly inducible promoter, the rate of spontaneous reversion was shown to increase when the mutant gene was highly transcribed. Thus, transcriptionally active DNA and enhanced spontaneous mutation rates are associated in yeast.


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