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Science 19 May 1995:
Vol. 268. no. 5213, pp. 1056 - 1060
DOI: 10.1126/science.7538698
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Articles

Science, Vol 268, Issue 5213, 1056-1060
Copyright © 1995 by American Association for the Advancement of Science

articles

Ribosomal RNA precursor processing by a eukaryotic U3 small nucleolar RNA-like molecule in an archaeon

S Potter, P Durovic, and PP Dennis

Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada.

An RNA-containing endonuclease that catalyzes the excision and maturation of the 16S ribosomal RNA (rRNA) from the rRNA primary transcript (pre-rRNA) in the hyperthermophilic archaeon Sulfolobus acidocaldarius has been characterized. The ribonucleoprotein was inactivated by micrococcal nuclease treatment and inactivation was reversed by reconstitution with bulk RNA. A 159-nucleotide RNA with sequence and structural similarity to U3 small nucleolar RNAs of eukaryotes copurified with the endonuclease activity. Oligonucleotide-targeted ribonuclease H inactivation of the U3-like RNA component also abolished processing activity. A motif within the U3 homolog is complementary to the region around the three cleavage sites in the pre-RNA substrate. Thus, U3-mediated processing of pre-rRNA is not specific to eukaryotes; its origin predates the divergence of archaea and eukaryotes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Ribosome Biogenesis: Role of Small Nucleolar RNA in Maturation of Eukaryotic rRNA.
S.A. GERBI, A.V. BOROVJAGIN, M. EZROKHI, and T.S. LANGE (2001)
Cold Spring Harb Symp Quant Biol 66, 575-590
   Abstract »    PDF »
Substrate Requirements for a Novel Archaeal Endonuclease That Cleaves Within the 5' External Transcribed Spacer of Sulfolobus acidocaldarius Precursor rRNA.
A. G. Russell, H. Ebhardt, and P. P. Dennis (1999)
Genetics 152, 1373-1385
   Abstract »    Full Text »
Archaebacteria Then ... Archaes Now (Are There Really No Archaeal Pathogens?).
J. N. Reeve (1999)
J. Bacteriol. 181, 3613-3617
   Full Text »


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