Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 19 May 1995:
Vol. 268. no. 5213, pp. 1049 - 1053
DOI: 10.1126/science.7754385
Prev | Table of Contents | Next

Articles

Science, Vol 268, Issue 5213, 1049-1053
Copyright © 1995 by American Association for the Advancement of Science

articles

Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences

K Weis, IW Mattaj, and AI Lamond

European Molecular Biology Laboratory, Heidelberg, Germany.

Import of proteins into the nucleus is a two-step process, involving nuclear localization sequence (NLS)-dependent docking of the substrate at the nuclear envelope followed by translocation through the nuclear pore. A recombinant human protein, hSRP1 alpha, bound in vitro specifically and directly to substrates containing either a simple or bipartite NLS motif. hSRP1 alpha promoted docking of import substrates to the nuclear envelope and together with recombinant human Ran reconstituted complete nuclear protein import. Thus, hSRP1 alpha has the properties of a cytosolic receptor for both simple and bipartite NLS motifs.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identification of a selective nuclear import signal in adenosine deaminases acting on RNA.
S. Maas and W. M. Gommans (2009)
Nucleic Acids Res. 37, 5822-5829
   Abstract »    Full Text »    PDF »
Role of Importin Alpha8, a New Member of the Importin Alpha Family of Nuclear Transport Proteins, in Early Embryonic Development in Cattle.
J. Tejomurtula, K.-B. Lee, S. K. Tripurani, G. W. Smith, and J. Yao (2009)
Biol Reprod 81, 333-342
   Abstract »    Full Text »    PDF »
Possible Role for Cellular Karyopherins in Regulating Polyomavirus and Papillomavirus Capsid Assembly.
G. Bird, M. O'Donnell, J. Moroianu, and R. L. Garcea (2008)
J. Virol. 82, 9848-9857
   Abstract »    Full Text »    PDF »
Molecular Basis for the Recognition of Snurportin 1 by Importin {beta}.
G. Mitrousis, A. S. Olia, N. Walker-Kopp, and G. Cingolani (2008)
J. Biol. Chem. 283, 7877-7884
   Abstract »    Full Text »    PDF »
Ebola Virus VP24 Proteins Inhibit the Interaction of NPI-1 Subfamily Karyopherin {alpha} Proteins with Activated STAT1.
St. P. Reid, C. Valmas, O. Martinez, F. M. Sanchez, and C. F. Basler (2007)
J. Virol. 81, 13469-13477
   Abstract »    Full Text »    PDF »
Role of the Sonchus Yellow Net Virus N Protein in Formation of Nuclear Viroplasms.
M. Deng, J. N. Bragg, S. Ruzin, D. Schichnes, D. King, M. M. Goodin, and A. O. Jackson (2007)
J. Virol. 81, 5362-5374
   Abstract »    Full Text »    PDF »
Nuclear Import of Bovine Papillomavirus Type 1 E1 Protein Is Mediated by Multiple Alpha Importins and Is Negatively Regulated by Phosphorylation near a Nuclear Localization Signal.
X.-L. Bian, G. Rosas-Acosta, Y.-C. Wu, and V. G. Wilson (2007)
J. Virol. 81, 2899-2908
   Abstract »    Full Text »    PDF »
Nuclear Localization Signal and Protein Context both Mediate Importin {alpha} Specificity of Nuclear Import Substrates.
B. Friedrich, C. Quensel, T. Sommer, E. Hartmann, and M. Kohler (2006)
Mol. Cell. Biol. 26, 8697-8709
   Abstract »    Full Text »    PDF »
Characterization of hCINAP, a Novel Coilin-interacting Protein Encoded by a Transcript from the Transcription Factor TAFIID32 Locus.
N. Santama, S. C. Ogg, A. Malekkou, S. E. Zographos, K. Weis, and A. I. Lamond (2005)
J. Biol. Chem. 280, 36429-36441
   Abstract »    Full Text »    PDF »
Dissection of a Novel Nuclear Localization Signal in Open Reading Frame 29 of Varicella-Zoster Virus.
C. L. Stallings and S. Silverstein (2005)
J. Virol. 79, 13070-13081
   Abstract »    Full Text »    PDF »
Cross-Talk between Snurportin1 Subdomains.
J. K. Ospina, G. B. Gonsalvez, J. Bednenko, E. Darzynkiewicz, L. Gerace, and A. G. Matera (2005)
Mol. Biol. Cell 16, 4660-4671
   Abstract »    Full Text »    PDF »
Importin-{alpha} Promotes Passage through the Nuclear Pore Complex of Human Immunodeficiency Virus Type 1 Vpr.
M. Kamata, Y. Nitahara-Kasahara, Y. Miyamoto, Y. Yoneda, and Y. Aida (2005)
J. Virol. 79, 3557-3564
   Abstract »    Full Text »    PDF »
Molecular Determinants for Subcellular Localization of Hepatitis C Virus Core Protein.
R. Suzuki, S. Sakamoto, T. Tsutsumi, A. Rikimaru, K. Tanaka, T. Shimoike, K. Moriishi, T. Iwasaki, K. Mizumoto, Y. Matsuura, et al. (2005)
J. Virol. 79, 1271-1281
   Abstract »    Full Text »    PDF »
The Positively Charged Termini of L2 Minor Capsid Protein Required for Bovine Papillomavirus Infection Function Separately in Nuclear Import and DNA Binding.
A. Fay, W. H. Yutzy IV, R. B. S. Roden, and J. Moroianu (2004)
J. Virol. 78, 13447-13454
   Abstract »    Full Text »    PDF »
In Vivo Analysis of Importin {alpha} Proteins Reveals Cellular Proliferation Inhibition and Substrate Specificity.
C. Quensel, B. Friedrich, T. Sommer, E. Hartmann, and M. Kohler (2004)
Mol. Cell. Biol. 24, 10246-10255
   Abstract »    Full Text »    PDF »
The L2 Minor Capsid Protein of Human Papillomavirus Type 16 Interacts with a Network of Nuclear Import Receptors.
M. S. Darshan, J. Lucchi, E. Harding, and J. Moroianu (2004)
J. Virol. 78, 12179-12188
   Abstract »    Full Text »    PDF »
Inhibition of Nuclear Import by the Proapoptotic Protein CC3.
F. W. King and E. Shtivelman (2004)
Mol. Cell. Biol. 24, 7091-7101
   Abstract »    Full Text »    PDF »
Protein Interactions Involved in Nuclear Import of the Agrobacterium VirE2 Protein in Vivo and in Vitro.
V. Citovsky, A. Kapelnikov, S. Oliel, N. Zakai, M. R. Rojas, R. L. Gilbertson, T. Tzfira, and A. Loyter (2004)
J. Biol. Chem. 279, 29528-29533
   Abstract »    Full Text »    PDF »
Impaired V(D)J Recombination and Lymphocyte Development in Core RAG1-expressing Mice.
D. D. Dudley, J. Sekiguchi, C. Zhu, M. J. Sadofsky, S. Whitlow, J. DeVido, R. J. Monroe, C. H. Bassing, and F. W. Alt (2003)
J. Exp. Med. 198, 1439-1450
   Abstract »    Full Text »    PDF »
Regulation of p120-catenin nucleocytoplasmic shuttling activity.
A. Roczniak-Ferguson and A. B. Reynolds (2003)
J. Cell Sci. 116, 4201-4212
   Abstract »    Full Text »    PDF »
A Nonconventional Nuclear Localization Signal within the UL84 Protein of Human Cytomegalovirus Mediates Nuclear Import via the Importin {alpha}/{beta} Pathway.
P. Lischka, G. Sorg, M. Kann, M. Winkler, and T. Stamminger (2003)
J. Virol. 77, 3734-3748
   Abstract »    Full Text »    PDF »
Nuclear Entry of High-Risk Human Papillomavirus Type 16 E6 Oncoprotein Occurs via Several Pathways.
L. G. Le Roux and J. Moroianu (2003)
J. Virol. 77, 2330-2337
   Abstract »    Full Text »    PDF »
Functional Proteomic Analysis of Human Nucleolus.
A. Scherl, Y. Coute, C. Deon, A. Calle, K. Kindbeiter, J.-C. Sanchez, A. Greco, D. Hochstrasser, and J.-J. Diaz (2002)
Mol. Biol. Cell 13, 4100-4109
   Abstract »    Full Text »    PDF »
Nuclear Import of Upf3p Is Mediated by Importin-{alpha}/-{beta} and Export to the Cytoplasm Is Required for a Functional Nonsense-Mediated mRNA Decay Pathway in Yeast.
R. L. Shirley, A. S. Ford, M. R. Richards, M. Albertini, and M. R. Culbertson (2002)
Genetics 161, 1465-1482
   Abstract »    Full Text »    PDF »
Nuclear Import Strategies of High Risk HPV16 L1 Major Capsid Protein.
L. M. Nelson, R. C. Rose, and J. Moroianu (2002)
J. Biol. Chem. 277, 23958-23964
   Abstract »    Full Text »    PDF »
Structure and function correlation in histone H2A peptide-mediated gene transfer.
D. Balicki, C. D. Putnam, P. V. Scaria, and E. Beutler (2002)
PNAS 99, 7467-7471
   Abstract »    Full Text »    PDF »
Characterization of an Unusual Importin alpha Binding Motif in the Borna Disease Virus p10 Protein That Directs Nuclear Import.
T. Wolff, G. Unterstab, G. Heins, J. A. Richt, and M. Kann (2002)
J. Biol. Chem. 277, 12151-12157
   Abstract »    Full Text »    PDF »
Characterization of the Nuclear Export Signal of Polypyrimidine Tract-binding Protein.
B. Li and T. S. B. Yen (2002)
J. Biol. Chem. 277, 10306-10314
   Abstract »    Full Text »    PDF »
Ribosomal Protein L12 Uses a Distinct Nuclear Import Pathway Mediated by Importin 11.
S. M. Plafker and I. G. Macara (2002)
Mol. Cell. Biol. 22, 1266-1275
   Abstract »    Full Text »    PDF »
The importin-{beta} binding domain of snurportin1 is responsible for the Ran- and energy-independent nuclear import of spliceosomal U snRNPs in vitro.
J. Huber, A. Dickmanns, and R. Luhrmann (2002)
J. Cell Biol. 156, 467-479
   Abstract »    Full Text »    PDF »
A developmentally regulated ARF-like 5 protein (ARL5), localized to nuclei and nucleoli, interacts with heterochromatin protein 1.
C.-Y. Lin, C.-C. Li, P.-H. Huang, and F.-J. S. Lee (2002)
J. Cell Sci. 115, 4433-4445
   Abstract »    Full Text »    PDF »
Transport into and out of the Nucleus.
I. G. Macara (2001)
Microbiol. Mol. Biol. Rev. 65, 570-594
   Abstract »    Full Text »    PDF »
MKP-7, a Novel Mitogen-activated Protein Kinase Phosphatase, Functions as a Shuttle Protein.
K. Masuda, H. Shima, M. Watanabe, and K. Kikuchi (2001)
J. Biol. Chem. 276, 39002-39011
   Abstract »    Full Text »    PDF »
Nuclear localization of the tyrosine kinase Itk and interaction of its SH3 domain with karyopherin {alpha} (Rch1{alpha}).
J. J. Perez-Villar, K. O'Day, D. H. Hewgill, S. G. Nadler, and S. B. Kanner (2001)
Int. Immunol. 13, 1265-1274
   Abstract »    Full Text »    PDF »
Mechanisms of Nuclear Import and Export That Control the Subcellular Localization of Class II Transactivator.
D. E. Cressman, W. J. O'Connor, S. F. Greer, X.-S. Zhu, and J. P.-Y. Ting (2001)
J. Immunol. 167, 3626-3634
   Abstract »    Full Text »    PDF »
Nup2p Dynamically Associates with the Distal Regions of the Yeast Nuclear Pore Complex.
D. J. Dilworth, A. Suprapto, J. C. Padovan, B. T. Chait, R. W. Wozniak, M. P. Rout, and J. D. Aitchison (2001)
J. Cell Biol. 153, 1465-1478
   Abstract »    Full Text »    PDF »
Cap methyltransferase selective binding and methylation of GpppG-RNA are stimulated by importin-{alpha}.
Y. Wen and A. J. Shatkin (2000)
Genes & Dev. 14, 2944-2949
   Abstract »    Full Text »
Nup2p, a Yeast Nucleoporin, Functions in Bidirectional Transport of Importin alpha.
J. Solsbacher, P. Maurer, F. Vogel, and G. Schlenstedt (2000)
Mol. Cell. Biol. 20, 8468-8479
   Abstract »    Full Text »
The potential terminase subunit of human cytomegalovirus, pUL56, is translocated into the nucleus by its own nuclear localization signal and interacts with importin {alpha}.
K. Giesen, K. Radsak, and E. Bogner (2000)
J. Gen. Virol. 81, 2231-2244
   Abstract »    Full Text »
The C-Terminal Proline-Rich Tail of Human Immunodeficiency Virus Type 2 Vpx Is Necessary for Nuclear Localization of the Viral Preintegration Complex in Nondividing Cells.
H. A. Pancio, N. Vander Heyden, and L. Ratner (2000)
J. Virol. 74, 6162-6167
   Abstract »    Full Text »
Relationship between Contact Inhibition and Intranuclear S100c of Normal Human Fibroblasts.
M. Sakaguchi, M. Miyazaki, Y. Inoue, T. Tsuji, H. Kouchi, T. Tanaka, H. Yamada, and M. Namba (2000)
J. Cell Biol. 149, 1193-1206
   Abstract »    Full Text »    PDF »
Gatekeepers of the Nucleus.
S. R. Wente (2000)
Science 288, 1374-1377
   Abstract »    Full Text »
Nuclear Import of the Ran Exchange Factor, Rcc1, Is Mediated by at Least Two Distinct Mechanisms.
M. E. Nemergut and I. G. Macara (2000)
J. Cell Biol. 149, 835-850
   Abstract »    Full Text »    PDF »
The Nuclear Import of RCC1 Requires a Specific Nuclear Localization Sequence Receptor, Karyopherin alpha 3/Qip.
B. Talcott and M. S. Moore (2000)
J. Biol. Chem. 275, 10099-10104
   Abstract »    Full Text »    PDF »
A Human Importin-beta Family Protein, Transportin-SR2, Interacts with the Phosphorylated RS Domain of SR Proteins.
M.-C. Lai, R.-I. Lin, S.-Y. Huang, C.-W. Tsai, and W.-Y. Tarn (2000)
J. Biol. Chem. 275, 7950-7957
   Abstract »    Full Text »    PDF »
An Atp-Dependent, Ran-Independent Mechanism for Nuclear Import of the U1a and U2b'' Spliceosome Proteins.
M. Hetzer and I. W. Mattaj (2000)
J. Cell Biol. 148, 293-304
   Abstract »    Full Text »    PDF »
RanBP3 Contains an Unusual Nuclear Localization Signal That Is Imported Preferentially by Importin-alpha 3.
K. Welch, J. Franke, M. Kohler, and I. G. Macara (1999)
Mol. Cell. Biol. 19, 8400-8411
   Abstract »    Full Text »    PDF »
A Bipartite Nuclear Localization Signal Is Required for p53 Nuclear Import Regulated by a Carboxyl-terminal Domain.
S.-H. Liang and M. F. Clarke (1999)
J. Biol. Chem. 274, 32699-32703
   Abstract »    Full Text »    PDF »
Evidence for Distinct Substrate Specificities of Importin alpha Family Members in Nuclear Protein Import.
M. Kohler, C. Speck, M. Christiansen, F. R. Bischoff, S. Prehn, H. Haller, D. Gorlich, and E. Hartmann (1999)
Mol. Cell. Biol. 19, 7782-7791
   Abstract »    Full Text »    PDF »
Kap104p-mediated Nuclear Import. NUCLEAR LOCALIZATION SIGNALS IN mRNA-BINDING PROTEINS AND THE ROLE OF RAN AND RNA.
D. C. Y. Lee and J. D. Aitchison (1999)
J. Biol. Chem. 274, 29031-29037
   Abstract »    Full Text »    PDF »
Nuclear Import of Plasmid DNA in Digitonin-permeabilized Cells Requires Both Cytoplasmic Factors and Specific DNA Sequences.
G. L. Wilson, B. S. Dean, G. Wang, and D. A. Dean (1999)
J. Biol. Chem. 274, 22025-22032
   Abstract »    Full Text »    PDF »
Nuclear Import of Sterol Regulatory Element-binding Protein-2, a Basic Helix-Loop-Helix-Leucine Zipper (bHLH-Zip)-containing Transcription Factor, Occurs through the Direct Interaction of Importin beta with HLH-Zip.
E. Nagoshi, N. Imamoto, R. Sato, and Y. Yoneda (1999)
Mol. Biol. Cell 10, 2221-2233
   Abstract »    Full Text »
A Test of the Cytosolic Apolipoprotein E Hypothesis Fails to Detect the Escape of Apolipoprotein E from the Endocytic Pathway into the Cytosol and Shows that Direct Expression of Apolipoprotein E in the Cytosol is Cytotoxic.
R. B. DeMattos, F. E. Thorngate, and D. L. Williams (1999)
J. Neurosci. 19, 2464-2473
   Abstract »    Full Text »    PDF »
beta -Catenin Can Be Transported into the Nucleus in a Ran-unassisted Manner.
F. Yokoya, N. Imamoto, T. Tachibana, and Y. Yoneda (1999)
Mol. Biol. Cell 10, 1119-1131
   Abstract »    Full Text »
Characterization of Ikappa Balpha Nuclear Import Pathway.
P. Turpin, R. T. Hay, and C. Dargemont (1999)
J. Biol. Chem. 274, 6804-6812
   Abstract »    Full Text »    PDF »
The Nucleoporin Nup153 Plays a Critical Role in Multiple Types of Nuclear Export.
K. S. Ullman, S. Shah, M. A. Powers, and D. J. Forbes (1999)
Mol. Biol. Cell 10, 649-664
   Abstract »    Full Text »
Discrimination between NL1- and NL2-Mediated Nuclear Localization of the Glucocorticoid Receptor.
J. G. A. Savory, B. Hsu, I. R. Laquian, W. Giffin, T. Reich, R. J. G. Hache, and Y. A. Lefebvre (1999)
Mol. Cell. Biol. 19, 1025-1037
   Abstract »    Full Text »    PDF »
Interactions between a Nuclear Transporter and a Subset of Nuclear Pore Complex Proteins Depend on Ran GTPase.
M. Seedorf, M. Damelin, J. Kahana, T. Taura, and P. A. Silver (1999)
Mol. Cell. Biol. 19, 1547-1557
   Abstract »    Full Text »    PDF »
Ran-dependent Signal-mediated Nuclear Import Does Not Require GTP Hydrolysis by Ran.
E. D. Schwoebel, B. Talcott, I. Cushman, and M. S. Moore (1998)
J. Biol. Chem. 273, 35170-35175
   Abstract »    Full Text »    PDF »
Regulation of NF-{kappa}B, AP-1, NFAT, and STAT1 Nuclear Import in T Lymphocytes by Noninvasive Delivery of Peptide Carrying the Nuclear Localization Sequence of NF-{kappa}B p50.
T. R. Torgerson, A. D. Colosia, J. P. Donahue, Y.-Z. Lin, and J. Hawiger (1998)
J. Immunol. 161, 6084-6092
   Abstract »    Full Text »    PDF »
Characterization of HIV-1 Vpr Nuclear Import: Analysis of Signals and Pathways.
Y. Jenkins, M. McEntee, K. Weis, and W. C. Greene (1998)
J. Cell Biol. 143, 875-885
   Abstract »    Full Text »    PDF »
Nuclear RNA export.
F. Stutz and M. Rosbash (1998)
Genes & Dev. 12, 3303-3319
   Full Text »
Determination of the Functional Domain Organization of the Importin {alpha} Nuclear Import Factor.
A. Herold, R. Truant, H. Wiegand, and B. R. Cullen (1998)
J. Cell Biol. 143, 309-318
   Abstract »    Full Text »    PDF »
Ran and Nuclear Transport.
M. S. Moore (1998)
J. Biol. Chem. 273, 22857-22860
   Full Text »    PDF »
Phosphorylation regulates association of the transcription factor Pho4 with its import receptor Pse1/Kap121.
A. Kaffman, N. M. Rank, and E. K. O'Shea (1998)
Genes & Dev. 12, 2673-2683
   Abstract »    Full Text »
Differential Importin-alpha Recognition and Nuclear Transport by Nuclear Localization Signals within the High-Mobility-Group DNA Binding Domains of Lymphoid Enhancer Factor 1 and T-Cell Factor 1.
M. G. Prieve, K. L. Guttridge, J. Munguia, and M. L. Waterman (1998)
Mol. Cell. Biol. 18, 4819-4832
   Abstract »    Full Text »
Functional Conservation of the Transportin Nuclear Import Pathway in Divergent Organisms.
M. C. Siomi, M. Fromont, J.-C. Rain, L. Wan, F. Wang, P. Legrain, and G. Dreyfuss (1998)
Mol. Cell. Biol. 18, 4141-4148
   Abstract »    Full Text »
CNI-H0294, a Nuclear Importation Inhibitor of the Human Immunodeficiency Virus Type 1 Genome, Abrogates Virus Replication in Infected Activated Peripheral Blood Mononuclear Cells.
O. K. Haffar, M. D. Smithgall, S. Popov, P. Ulrich, A. G. Bruce, S. G. Nadler, A. Cerami, and M. I. Bukrinsky (1998)
Antimicrob. Agents Chemother. 42, 1133-1138
   Abstract »    Full Text »
Hepatitis Delta Antigen Mediates the Nuclear Import of Hepatitis Delta Virus RNA.
H.-C. Chou, T.-Y. Hsieh, G.-T. Sheu, and M. M. C. Lai (1998)
J. Virol. 72, 3684-3690
   Abstract »    Full Text »    PDF »
Nuclear Localization of Ikappa Balpha Is Mediated by the Second Ankyrin Repeat: the Ikappa Balpha Ankyrin Repeats Define a Novel Class of cis-Acting Nuclear Import Sequences.
S. Sachdev, A. Hoffmann, and M. Hannink (1998)
Mol. Cell. Biol. 18, 2524-2534
   Abstract »    Full Text »
Identification of a Novel Cytoplasmic Protein That Specifically Binds to Nuclear Localization Signal Motifs.
S. Li, C.-Y. Ku, A. A. Farmer, Y.-S. Cong, C.-F. Chen, and W.-H. Lee (1998)
J. Biol. Chem. 273, 6183-6189
   Abstract »    Full Text »    PDF »
Overexpression of the Nucleoporin CAN/NUP214 Induces Growth Arrest, Nucleocytoplasmic Transport Defects, and Apoptosis.
J. Boer, J. Bonten-Surtel, and G. Grosveld (1998)
Mol. Cell. Biol. 18, 1236-1247
   Abstract »    Full Text »
Identification and Functional Characterization of a Novel Nuclear Localization Signal Present in the Yeast Nab2 Poly(A)+ RNA Binding Protein.
R. Truant, R. A. Fridell, R. E. Benson, H. Bogerd, and B. R. Cullen (1998)
Mol. Cell. Biol. 18, 1449-1458
   Abstract »    Full Text »
Nuclear Localization and Export Signals of the Human Aryl Hydrocarbon Receptor.
T. Ikuta, H. Eguchi, T. Tachibana, Y. Yoneda, and K. Kawajiri (1998)
J. Biol. Chem. 273, 2895-2904
   Abstract »    Full Text »    PDF »
The role of the ran GTPase in nuclear assembly and DNA replication: characterisation of the effects of Ran mutants.
M Hughes, C Zhang, J. Avis, C. Hutchison, and P. Clarke (1998)
J. Cell Sci. 111, 3017-3026
   Abstract »    PDF »
A T42A Ran Mutation: Differential Interactions with Effectors and Regulators, and Defect in Nuclear Protein Import.
G. A. Murphy, M. S. Moore, G. Drivas, P. P. de la Ossa, A. Villamarin, P. D'Eustachio, and M. G. Rush (1997)
Mol. Biol. Cell 8, 2591-2604
   Abstract »    Full Text »
Ran-unassisted Nuclear Migration of a 97-kD Component of Nuclear Pore-targeting Complex.
S. Kose, N. Imamoto, T. Tachibana, T. Shimamoto, and Y. Yoneda (1997)
J. Cell Biol. 139, 841-849
   Abstract »    Full Text »    PDF »
Nucleocytoplasmic Recycling of the Nuclear Localization Signal Receptor {alpha} Subunit In Vivo Is Dependent on a Nuclear Export Signal, Energy, and RCC1.
I. Boche and E. Fanning (1997)
J. Cell Biol. 139, 313-325
   Abstract »    Full Text »    PDF »
Differential Modes of Nuclear Localization Signal (NLS) Recognition by Three Distinct Classes of NLS Receptors.
Y. Miyamoto, N. Imamoto, T. Sekimoto, T. Tachibana, T. Seki, S. Tada, T. Enomoto, and Y. Yoneda (1997)
J. Biol. Chem. 272, 26375-26381
   Abstract »    Full Text »    PDF »
Evidence for a Role of CRM1 in Signal-Mediated Nuclear Protein Export.
B. Ossareh-Nazari, F. Bachelerie, and C. Dargemont (1997)
Science 278, 141-144
   Abstract »    Full Text »
Nuclear localization signal binding protein from Arabidopsis mediates nuclear import of Agrobacterium VirD2 protein.
N. Ballas and V. Citovsky (1997)
PNAS 94, 10723-10728
   Abstract »    Full Text »    PDF »
Transportin-mediated Nuclear Import of Heterogeneous Nuclear RNP Proteins.
M. C. Siomi, P. S. Eder, N. Kataoka, L. Wan, Q. Liu, and G. Dreyfuss (1997)
J. Cell Biol. 138, 1181-1192
   Abstract »    Full Text »    PDF »
RanGTP-mediated nuclear export of karyopherin alpha  involves its interaction with the nucleoporin Nup153.
J. Moroianu, G. Blobel, and A. Radu (1997)
PNAS 94, 9699-9704
   Abstract »    Full Text »    PDF »
Association of the T-cell Protein Tyrosine Phosphatase with Nuclear Import Factor p97.
T. Tiganis, A. J. Flint, S. A. Adam, and N. K. Tonks (1997)
J. Biol. Chem. 272, 21548-21557
   Abstract »    Full Text »    PDF »
Disassembly of RanGTP-Karyopherin beta  Complex, an Intermediate in Nuclear Protein Import.
M. Floer, G. Blobel, and M. Rexach (1997)
J. Biol. Chem. 272, 19538-19546
   Abstract »    Full Text »    PDF »
A Novel Class of RanGTP Binding Proteins.
D. Gorlich, M. Dabrowski, F. R. Bischoff, U. Kutay, P. Bork, E. Hartmann, S. Prehn, and E. Izaurralde (1997)
J. Cell Biol. 138, 65-80
   Abstract »    Full Text »    PDF »
A Nuclear Localization Signal of Human Aryl Hydrocarbon Receptor Nuclear Translocator/Hypoxia-inducible Factor 1beta Is a Novel Bipartite Type Recognized by the Two Components of Nuclear Pore-targeting Complex.
H. Eguchi, T. Ikuta, T. Tachibana, Y. Yoneda, and K. Kawajiri (1997)
J. Biol. Chem. 272, 17640-17647
   Abstract »    Full Text »    PDF »
The Protein Kinase CK2 Site (Ser111/112) Enhances Recognition of the Simian Virus 40 Large T-antigen Nuclear Localization Sequence by Importin.
S. Hubner, C.-Y. Xiao, and D. A. Jans (1997)
J. Biol. Chem. 272, 17191-17195
   Abstract »    Full Text »    PDF »
A Nuclear Export Signal in Kap95p Is Required for Both Recycling the Import Factor and Interaction with the Nucleoporin GLFG Repeat Regions of Nup116p and Nup100p.
M. K. Iovine and S. R. Wente (1997)
J. Cell Biol. 137, 797-811
   Abstract »    Full Text »    PDF »
Karyopherin beta 2 mediates nuclear import of a mRNA binding protein.
N. Bonifaci, J. Moroianu, A. Radu, and G. Blobel (1997)
PNAS 94, 5055-5060
   Abstract »    Full Text »    PDF »
Cloning and characterization of human karyopherin beta 3.
N. R. Yaseen and G. Blobel (1997)
PNAS 94, 4451-4456
   Abstract »    Full Text »    PDF »
Different Binding Domains for Ran-GTP and Ran-GDP/RanBP1 on Nuclear Import Factor p97.
N. C. Chi, E. J.H. Adam, and S. A. Adam (1997)
J. Biol. Chem. 272, 6818-6822
   Abstract »    Full Text »    PDF »
Differential Expression and Sequence-specific Interaction of Karyopherin alpha with Nuclear Localization Sequences.
S. G. Nadler, D. Tritschler, O. K. Haffar, J. Blake, A. G. Bruce, and J. S. Cleaveland (1997)
J. Biol. Chem. 272, 4310-4315
   Abstract »    Full Text »    PDF »
Nuclear import of hnRNP A1 is mediated by a novel cellular cofactor related to karyopherin-beta.
R. Fridell, R Truant, L Thorne, R. Benson, and B. Cullen (1997)
J. Cell Sci. 110, 1325-1331
   Abstract »    PDF »
Ran-binding Protein 1(RanBP1) Forms a Ternary Complex with Ran and Karyopherin beta and Reduces Ran GTPase-activating Protein (RanGAP) Inhibition by Karyopherin beta.
K. M. Lounsbury and I. G. Macara (1997)
J. Biol. Chem. 272, 551-555
   Abstract »    Full Text »    PDF »
The Nuclear Localization Sequences of the BRCA1 Protein Interact with the Importin-alpha Subunit of the Nuclear Transport Signal Receptor.
C.-F. Chen, S. Li, Y. Chen, P.-L. Chen, Z. D. Sharp, and W.-H. Lee (1996)
J. Biol. Chem. 271, 32863-32868
   Abstract »    Full Text »    PDF »
Interferon-gamma -dependent Nuclear Import of Stat1 Is Mediated by the GTPase Activity of Ran/TC4.
T. Sekimoto, K. Nakajima, T. Tachibana, T. Hirano, and Y. Yoneda (1996)
J. Biol. Chem. 271, 31017-31020
   Abstract »    Full Text »    PDF »
Expression in yeast of binding regions of karyopherins alpha  and beta  inhibits nuclear import and cell growth.
C. Enenkel, N. Schulke, and G. Blobel (1996)
PNAS 93, 12986-12991
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)