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Science 5 May 1995: Vol. 268. no. 5211, pp. 720 - 722 DOI: 10.1126/science.7537388
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Articles
Science, Vol 268, Issue 5211, 720-722
Copyright © 1995 by American Association for the Advancement of Science
Generation of memory B cells and plasma cells in vitro
C Arpin,
J Dechanet,
C Van Kooten,
P Merville,
G Grouard,
F Briere,
J Banchereau,
and
YJ Liu
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
After germinal center B cells undergo somatic mutation and antigen selection, they become either memory B cells or plasma cells, but the signal requirements that control entry into either pathway have been unclear. When purified human germinal center cells were cultured with interleukin-2, interleukin-10, and cells expressing CD40 ligand, cells with characteristics of memory B cells were generated. Removal of CD40 ligand from the system resulted in terminal differentiation of germinal center B cells into cells with the characteristics of plasma cells. These results indicate that CD40 ligand directs the differentiation of germinal center B cells toward memory B cells rather than toward plasma cells.
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- BAFF, a Novel Ligand of the Tumor Necrosis Factor Family, Stimulates B Cell Growth.
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- Detection of Functional Platelet-Activating Factor Receptors on Human Tonsillar B Lymphocytes.
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162, 5498-5505
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- Differential Responses to CD40 Ligation Among Burkitt Lymphoma Lines That Are Uniformly Responsive to Epstein-Barr Virus Latent Membrane Protein 1.
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162, 3298-3307
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- Toward a Role of Dendritic Cells in the Germinal Center Reaction: Triggering of B Cell Proliferation and Isotype Switching.
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162, 3428-3436
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- Differential Regulation of Gene Transcription in Subpopulations of Human B Lymphocytes by Vitamin D3.
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Endocrinology
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- Light Chain Shifting: Identification of a Human Plasma Cell Line Actively Undergoing Light Chain Replacement.
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Blood
93, 198-207
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- CD27/CD70 Interaction Augments IgE Secretion by Promoting the Differentiation of Memory B Cells into Plasma Cells.
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161, 6496-6502
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- In Vitro Reconstitution of Human B-Cell Ontogeny: From CD34+ Multipotent Progenitors to Ig-Secreting Cells.
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Blood
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- OPG/FDCR-1, a TNF Receptor Family Member, Is Expressed in Lymphoid Cells and Is Up-Regulated by Ligating CD40.
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- Nerve Growth Factor Released by CD40 Ligand-Transfected L Cells: Implications for Functional and Phenotypic Studies on CD40+ Cells.
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Blood
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- CD40 Engagement Triggers Switching to IgA1 and IgA2 in Human B Cells Through Induction of Endogenous TGF-{beta}: Evidence for TGF-{beta} But Not IL-10-Dependent Direct S{micro}->S{alpha} and Sequential S{micro}->S{gamma}, S{gamma}->S{alpha} DNA Recombination.
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- Identification of Functional Human Splenic Memory B Cells by Expression of CD148 and CD27.
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- Transfer of Primitive Stem/Progenitor Bone Marrow Cells from LT{alpha}-/- Donors to Wild-Type Hosts: Implications for the Generation of Architectural Events in Lymphoid B Cell Domains.
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- c-Fos Induces Apoptosis in Germinal Center B Cells.
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- Prolonged Phenotypic, Functional, and Molecular Change in Group I Burkitt Lymphoma Cells on Short-Term Exposure to CD40 Ligand.
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Blood
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- Critical Role of IL-12 in Dendritic Cell-Induced Differentiation of Naive B Lymphocytes.
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- Antigen Receptor Engagement Turns off the V(D)J Recombination Machinery in Human Tonsil B Cells.
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- The A-Myb Transcription Factor Is a Marker of Centroblasts In Vivo.
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