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Science 5 May 1995:
Vol. 268. no. 5211, pp. 720 - 722
DOI: 10.1126/science.7537388

Articles

Science, Vol 268, Issue 5211, 720-722
Copyright © 1995 by American Association for the Advancement of Science


articles

Generation of memory B cells and plasma cells in vitro

C Arpin, J Dechanet, C Van Kooten, P Merville, G Grouard, F Briere, J Banchereau, and YJ Liu

Schering-Plough, Laboratory for Immunological Research, Dardilly, France.

After germinal center B cells undergo somatic mutation and antigen selection, they become either memory B cells or plasma cells, but the signal requirements that control entry into either pathway have been unclear. When purified human germinal center cells were cultured with interleukin-2, interleukin-10, and cells expressing CD40 ligand, cells with characteristics of memory B cells were generated. Removal of CD40 ligand from the system resulted in terminal differentiation of germinal center B cells into cells with the characteristics of plasma cells. These results indicate that CD40 ligand directs the differentiation of germinal center B cells toward memory B cells rather than toward plasma cells.


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Blood 93, 198-207
   Abstract »    Full Text »    PDF »
CD27/CD70 Interaction Augments IgE Secretion by Promoting the Differentiation of Memory B Cells into Plasma Cells.
H. Nagumo, K. Agematsu, K. Shinozaki, S. Hokibara, S. Ito, M. Takamoto, T. Nikaido, K. Yasui, Y. Uehara, A. Yachie, et al. (1998)
J. Immunol. 161, 6496-6502
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In Vitro Reconstitution of Human B-Cell Ontogeny: From CD34+ Multipotent Progenitors to Ig-Secreting Cells.
A.-C. Fluckiger, E. Sanz, M. Garcia-Lloret, T. Su, Q.-L. Hao, R. Kato, S. Quan, A. de la Hera, G. M. Crooks, O. N. Witte, et al. (1998)
Blood 92, 4509-4520
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OPG/FDCR-1, a TNF Receptor Family Member, Is Expressed in Lymphoid Cells and Is Up-Regulated by Ligating CD40.
T. J. Yun, P. M. Chaudhary, G. L. Shu, J. K. Frazer, M. K. Ewings, S. M. Schwartz, V. Pascual, L. E. Hood, and E. A. Clark3 (1998)
J. Immunol. 161, 6113-6121
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Nerve Growth Factor Released by CD40 Ligand-Transfected L Cells: Implications for Functional and Phenotypic Studies on CD40+ Cells.
A. De Milito, N. Nagy, A. Samuelsson, F. Chiodi, and E. Rajnavolgyi (1998)
Blood 92, 4482-4484
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CD40 Engagement Triggers Switching to IgA1 and IgA2 in Human B Cells Through Induction of Endogenous TGF-{beta}: Evidence for TGF-{beta} But Not IL-10-Dependent Direct S{micro}->S{alpha} and Sequential S{micro}->S{gamma}, S{gamma}->S{alpha} DNA Recombination.
H. Zan, A. Cerutti, P. Dramitinos, A. Schaffer, and P. Casali (1998)
J. Immunol. 161, 5217-5225
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Identification of Functional Human Splenic Memory B Cells by Expression of CD148 and CD27.
S. G. Tangye, Y.-J. Liu, G. Aversa, J. H. Phillips, and J. E. de Vries (1998)
J. Exp. Med. 188, 1691-1703
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Transfer of Primitive Stem/Progenitor Bone Marrow Cells from LT{alpha}-/- Donors to Wild-Type Hosts: Implications for the Generation of Architectural Events in Lymphoid B Cell Domains.
R. E. Mebius, S. van Tuijl, I. L. Weissman, and T. D. Randall (1998)
J. Immunol. 161, 3836-3843
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c-Fos Induces Apoptosis in Germinal Center B Cells.
K. Inada, S. Okada, J. Phuchareon, M. Hatano, T. Sugimoto, H. Moriya, and T. Tokuhisa (1998)
J. Immunol. 161, 3853-3861
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Prolonged Phenotypic, Functional, and Molecular Change in Group I Burkitt Lymphoma Cells on Short-Term Exposure to CD40 Ligand.
M. P. Baker, A. G. Eliopoulos, L. S. Young, R. J. Armitage, C. D. Gregory, and J. Gordon (1998)
Blood 92, 2830-2843
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Critical Role of IL-12 in Dendritic Cell-Induced Differentiation of Naive B Lymphocytes.
B. Dubois, C. Massacrier, B. Vanbervliet, J. Fayette, F. Briere, J. Banchereau, and C. Caux (1998)
J. Immunol. 161, 2223-2231
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Antigen Receptor Engagement Turns off the V(D)J Recombination Machinery in Human Tonsil B Cells.
E. Meffre, F. Papavasiliou, P. Cohen, O. de Bouteiller, D. Bell, H. Karasuyama, C. Schiff, J. Banchereau, Y.-J. Liu, and M. C. Nussenzweig (1998)
J. Exp. Med. 188, 765-772
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The A-Myb Transcription Factor Is a Marker of Centroblasts In Vivo.
J. Golay, V. Broccoli, G. Lamorte, C. Bifulco, C. Parravicini, A. Pizzey, N. S. B. Thomas, D. Delia, P. Ferrauti, D. Vitolo, et al. (1998)
J. Immunol. 160, 2786-2793
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