Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 14 April 1995:
Vol. 268. no. 5208, pp. 284 - 286
DOI: 10.1126/science.7716520
Prev | Table of Contents | Next

Articles

Science, Vol 268, Issue 5208, 284-286
Copyright © 1995 by American Association for the Advancement of Science

articles

Killing of trypanosomes by the human haptoglobin-related protein

AB Smith, JD Esko, and SL Hajduk

Department of Biochemistry and Molecular Genetics, School of Medicine and Dentistry, University of Alabama at Birmingham 35294, USA.

African trypanosomes cause disease in humans and animals. Trypanosoma brucei brucei affects cattle but not humans because of its sensitivity to a subclass of human high density lipoproteins (HDLs) called trypanosome lytic factor (TLF). TLF contains two apolipoproteins that are sufficient to cause lysis of T. b. brucei in vitro. These proteins were identified as the human haptoglobin-related protein and paraoxonase-arylesterase. An antibody to haptoglobin inhibited TLF activity. TLF was shown to exhibit peroxidase activity and to be inhibited by catalase. These results suggest that TLF kills trypanosomes by oxidative damage initiated by its peroxidase activity.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Receptor targeting of hemoglobin mediated by the haptoglobins: roles beyond heme scavenging.
M. J. Nielsen and S. K. Moestrup (2009)
Blood 114, 764-771
   Abstract »    Full Text »    PDF »
Membrane Permeabilization by Trypanosome Lytic Factor, a Cytolytic Human High Density Lipoprotein.
J. M. Harrington, S. Howell, and S. L. Hajduk (2009)
J. Biol. Chem. 284, 13505-13512
   Abstract »    Full Text »    PDF »
Distinct roles of apolipoprotein components within the trypanosome lytic factor complex revealed in a novel transgenic mouse model.
M. P. Molina-Portela, M. Samanovic, and J. Raper (2008)
J. Exp. Med. 205, 1721-1728
   Abstract »    Full Text »    PDF »
An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma.
O. Axler, J. Ahnstrom, and B. Dahlback (2007)
J. Lipid Res. 48, 1772-1780
   Abstract »    Full Text »    PDF »
Distinct roles of haptoglobin-related protein and apolipoprotein L-I in trypanolysis by human serum.
B. Vanhollebeke, M. J. Nielsen, Y. Watanabe, P. Truc, L. Vanhamme, K. Nakajima, S. K. Moestrup, and E. Pays (2007)
PNAS 104, 4118-4123
   Abstract »    Full Text »    PDF »
A Unique Loop Extension in the Serine Protease Domain of Haptoglobin Is Essential for CD163 Recognition of the Haptoglobin-Hemoglobin Complex.
M. J. Nielsen, S. V. Petersen, C. Jacobsen, S. Thirup, J. J. Enghild, J. H. Graversen, and S. K. Moestrup (2007)
J. Biol. Chem. 282, 1072-1079
   Abstract »    Full Text »    PDF »
Haptoglobin-related protein is a high-affinity hemoglobin-binding plasma protein.
M. J. Nielsen, S. V. Petersen, C. Jacobsen, C. Oxvig, D. Rees, H. J. Moller, and S. K. Moestrup (2006)
Blood 108, 2846-2849
   Abstract »    Full Text »    PDF »
In Vitro Generation of Human High-Density-Lipoprotein-Resistant Trypanosoma brucei brucei..
S. D. Faulkner, M. W. Oli, R. Kieft, L. Cotlin, J. Widener, A. Shiflett, M. J. Cipriano, S. E. Pacocha, S. R. Birkeland, S. L. Hajduk, et al. (2006)
Eukaryot. Cell 5, 1276-1286
   Abstract »    Full Text »    PDF »
Serum Resistance-Associated Protein Blocks Lysosomal Targeting of Trypanosome Lytic Factor in Trypanosoma brucei.
M. W. Oli, L. F. Cotlin, A. M. Shiflett, and S. L. Hajduk (2006)
Eukaryot. Cell 5, 132-139
   Abstract »    Full Text »    PDF »
Human High Density Lipoproteins Are Platforms for the Assembly of Multi-component Innate Immune Complexes.
A. M. Shiflett, J. R. Bishop, A. Pahwa, and S. L. Hajduk (2005)
J. Biol. Chem. 280, 32578-32585
   Abstract »    Full Text »    PDF »
Evidence for a Trypanosoma brucei Lipoprotein Scavenger Receptor.
H. P. Green, M. del Pilar Molina Portela, E. N. St. Jean, E. B. Lugli, and J. Raper (2003)
J. Biol. Chem. 278, 422-427
   Abstract »    Full Text »    PDF »
Human Serum Paraoxonase/Arylesterase's Retained Hydrophobic N-Terminal Leader Sequence Associates With HDLs by Binding Phospholipids : Apolipoprotein A-I Stabilizes Activity.
R. C. Sorenson, C. L. Bisgaier, M. Aviram, C. Hsu, S. Billecke, and B. N. La Du (1999)
Arterioscler Thromb Vasc Biol 19, 2214-2225
   Abstract »    Full Text »    PDF »
Characterization of a Novel Trypanosome Lytic Factor from Human Serum.
J. Raper, R. Fung, J. Ghiso, V. Nussenzweig, and S. Tomlinson (1999)
Infect. Immun. 67, 1910-1916
   Abstract »    Full Text »    PDF »
History of Sleeping Sickness in East Africa.
G. Hide (1999)
Clin. Microbiol. Rev. 12, 112-125
   Abstract »    Full Text »    PDF »
Trypanosoma brucei gamma -Glutamylcysteine Synthetase. CHARACTERIZATION OF THE KINETIC MECHANISM AND THE ROLE OF CYS-319 IN CYSTAMINE INACTIVATION.
D. L. Brekken and M. A. Phillips (1998)
J. Biol. Chem. 273, 26317-26322
   Abstract »    Full Text »    PDF »
Characterization of the Human Serum Trypanosome Toxin, Haptoglobin-related Protein.
M. Muranjan, V. Nussenzweig, and S. Tomlinson (1998)
J. Biol. Chem. 273, 3884-3887
   Abstract »    Full Text »    PDF »
Plasma Lipopolysaccharide-binding Protein Is Found Associated with a Particle Containing Apolipoprotein A-I, Phospholipid, and Factor H-related Proteins.
C. T. Park and S. D. Wright (1996)
J. Biol. Chem. 271, 18054-18060
   Abstract »    Full Text »    PDF »
Characterization of Trypanosoma brucei gamma -Glutamylcysteine Synthetase, an Essential Enzyme in the Biosynthesis of Trypanothione (Diglutathionylspermidine).
D. V. Lueder and M. A. Phillips (1996)
J. Biol. Chem. 271, 17485-17490
   Abstract »    Full Text »    PDF »
Low temperature reversibly inhibits transport from tubular endosomes to a perinuclear, acidic compartment in African trypanosomes.
M. Brickman, J. Cook, and A. Balber (1995)
J. Cell Sci. 108, 3611-3621
   Abstract »    PDF »
Haptoglobin-related Protein Mediates Trypanosome Lytic Factor Binding to Trypanosomes.
J. Drain, J. R. Bishop, and S. L. Hajduk (2001)
J. Biol. Chem. 276, 30254-30260
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)