Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 7 April 1995:
Vol. 268. no. 5207, pp. 103 - 106
DOI: 10.1126/science.7701329
Prev | Table of Contents | Next

Articles

Science, Vol 268, Issue 5207, 103-106
Copyright © 1995 by American Association for the Advancement of Science

articles

Structure of the cell wall anchor of surface proteins in Staphylococcus aureus

O Schneewind, A Fowler, and KF Faull

Department of Microbiology and Immunology, University of California School of Medicine, Los Angeles 90024, USA.

Many surface proteins are anchored to the cell wall of Gram-positive bacteria and are involved in the pathogenesis of these organisms. A hybrid molecule was designed that, when expressed in Staphylococcus aureus, was anchored to the cell wall and could be released by controlled enzymatic digestion. By a combination of molecular biology and mass spectrometry techniques, the structure of the cell wall anchor of surface proteins in S. aureus was revealed. After cleavage of surface proteins between threonine and glycine of the conserved LPXTG motif, the carboxyl of threonine is amide-linked to the free amino group of the pentaglycine crossbridge in the staphylococcal cell wall.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A Straight Path to Circular Proteins.
J. M. Antos, M. W.-L. Popp, R. Ernst, G.-L. Chew, E. Spooner, and H. L. Ploegh (2009)
J. Biol. Chem. 284, 16028-16036
   Abstract »    Full Text »    PDF »
Sortase D Forms the Covalent Bond That Links BcpB to the Tip of Bacillus cereus Pili.
J. M. Budzik, S.-Y. Oh, and O. Schneewind (2009)
J. Biol. Chem. 284, 12989-12997
   Abstract »    Full Text »    PDF »
Protein A-Mediated Multicellular Behavior in Staphylococcus aureus.
N. Merino, A. Toledo-Arana, M. Vergara-Irigaray, J. Valle, C. Solano, E. Calvo, J. A. Lopez, T. J. Foster, J. R. Penades, and I. Lasa (2009)
J. Bacteriol. 191, 832-843
   Abstract »    Full Text »    PDF »
Cell Wall Anchor Structure of BcpA Pili in Bacillus anthracis.
J. M. Budzik, S.-Y. Oh, and O. Schneewind (2008)
J. Biol. Chem. 283, 36676-36686
   Abstract »    Full Text »    PDF »
Isolation and characterization of {alpha}-enolase, a novel fibronectin-binding protein from Streptococcus suis.
M. Esgleas, Y. Li, M. A. Hancock, J. Harel, J. D. Dubreuil, and M. Gottschalk (2008)
Microbiology 154, 2668-2679
   Abstract »    Full Text »    PDF »
Amide bonds assemble pili on the surface of bacilli.
J. M. Budzik, L. A. Marraffini, P. Souda, J. P. Whitelegge, K. F. Faull, and O. Schneewind (2008)
PNAS 105, 10215-10220
   Abstract »    Full Text »    PDF »
Sortase as a Target of Anti-Infective Therapy.
A. W. Maresso and O. Schneewind (2008)
Pharmacol. Rev. 60, 128-141
   Abstract »    Full Text »    PDF »
System Using Tandem Repeats of the cA Peptidoglycan-Binding Domain from Lactococcus lactis for Display of both N- and C-Terminal Fusions on Cell Surfaces of Lactic Acid Bacteria.
K. Okano, Q. Zhang, S. Kimura, J. Narita, T. Tanaka, H. Fukuda, and A. Kondo (2008)
Appl. Envir. Microbiol. 74, 1117-1123
   Abstract »    Full Text »    PDF »
Lysostaphin as a treatment for systemic Staphylococcus aureus infection in a mouse model.
J. F. Kokai-Kun, T. Chanturiya, and J. J. Mond (2007)
J. Antimicrob. Chemother. 60, 1051-1059
   Abstract »    Full Text »    PDF »
Evaluation of Staphylococcal Cell Surface Display and Flow Cytometry for Postselectional Characterization of Affinity Proteins in Combinatorial Protein Engineering Applications.
J. Lofblom, J. Sandberg, H. Wernerus, and S. Stahl (2007)
Appl. Envir. Microbiol. 73, 6714-6721
   Abstract »    Full Text »    PDF »
Sortase C-Mediated Anchoring of BasI to the Cell Wall Envelope of Bacillus anthracis.
L. A. Marraffini and O. Schneewind (2007)
J. Bacteriol. 189, 6425-6436
   Abstract »    Full Text »    PDF »
Activation of Inhibitors by Sortase Triggers Irreversible Modification of the Active Site.
A. W. Maresso, R. Wu, J. W. Kern, R. Zhang, D. Janik, D. M. Missiakas, M.-E. Duban, A. Joachimiak, and O. Schneewind (2007)
J. Biol. Chem. 282, 23129-23139
   Abstract »    Full Text »    PDF »
Distribution of Protein A on the Surface of Staphylococcus aureus.
A. C. DeDent, M. McAdow, and O. Schneewind (2007)
J. Bacteriol. 189, 4473-4484
   Abstract »    Full Text »    PDF »
C-Terminal WxL Domain Mediates Cell Wall Binding in Enterococcus faecalis and Other Gram-Positive Bacteria.
S. Brinster, S. Furlan, and P. Serror (2007)
J. Bacteriol. 189, 1244-1253
   Abstract »    Full Text »    PDF »
Lysostaphin-Resistant Variants of Staphylococcus aureus Demonstrate Reduced Fitness In Vitro and In Vivo.
C. Kusuma, A. Jadanova, T. Chanturiya, and J. F. Kokai-Kun (2007)
Antimicrob. Agents Chemother. 51, 475-482
   Abstract »    Full Text »    PDF »
High-Affinity Binding of the Staphylococcal HarA Protein to Haptoglobin and Hemoglobin Involves a Domain with an Antiparallel Eight-Stranded {beta}-Barrel Fold.
A. Dryla, B. Hoffmann, D. Gelbmann, C. Giefing, M. Hanner, A. Meinke, A. S. Anderson, W. Koppensteiner, R. Konrat, A. von Gabain, et al. (2007)
J. Bacteriol. 189, 254-264
   Abstract »    Full Text »    PDF »
Variable expression of immunoreactive surface proteins of Propionibacterium acnes.
M. J. Lodes, H. Secrist, D. R. Benson, S. Jen, K. D. Shanebeck, J. Guderian, J.-F. Maisonneuve, A. Bhatia, D. Persing, S. Patrick, et al. (2006)
Microbiology 152, 3667-3681
   Abstract »    Full Text »    PDF »
Vaccine assembly from surface proteins of Staphylococcus aureus.
Y. K. Stranger-Jones, T. Bae, and O. Schneewind (2006)
PNAS 103, 16942-16947
   Abstract »    Full Text »    PDF »
Endopeptidase and glycosidase activities of the bacteriophage b30 lysin..
J. R. Baker, C. Liu, S. Dong, and D. G. Pritchard (2006)
Appl. Envir. Microbiol. 72, 6825-6828
   Abstract »    Full Text »    PDF »
Staphylococcus aureus Mutants with Increased Lysostaphin Resistance..
A. Grundling, D. M. Missiakas, and O. Schneewind (2006)
J. Bacteriol. 188, 6286-6297
   Abstract »    Full Text »    PDF »
SdrI, a Serine-Aspartate Repeat Protein Identified in Staphylococcus saprophyticus Strain 7108, Is a Collagen-Binding Protein..
T. Sakinc, B. Kleine, and S. G. Gatermann (2006)
Infect. Immun. 74, 4615-4623
   Abstract »    Full Text »    PDF »
Sortases and the Art of Anchoring Proteins to the Envelopes of Gram-Positive Bacteria.
L. A. Marraffini, A. C. DeDent, and O. Schneewind (2006)
Microbiol. Mol. Biol. Rev. 70, 192-221
   Abstract »    Full Text »    PDF »
Novel Surface Display System for Proteins on Non-Genetically Modified Gram-Positive Bacteria.
T. Bosma, R. Kanninga, J. Neef, S. A. L. Audouy, M. L. van Roosmalen, A. Steen, G. Buist, J. Kok, O. P. Kuipers, G. Robillard, et al. (2006)
Appl. Envir. Microbiol. 72, 880-889
   Abstract »    Full Text »    PDF »
Structure of the streptococcal cell wall C5a peptidase.
C. K. Brown, Z.-Y. Gu, Y. V. Matsuka, S. S. Purushothaman, L. A. Winter, P. P. Cleary, S. B. Olmsted, D. H. Ohlendorf, and C. A. Earhart (2005)
PNAS 102, 18391-18396
   Abstract »    Full Text »    PDF »
Unconventional Mode of Attachment of the Ruminococcus flavefaciens Cellulosome to the Cell Surface.
M. T. Rincon, T. Cepeljnik, J. C. Martin, R. Lamed, Y. Barak, E. A. Bayer, and H. J. Flint (2005)
J. Bacteriol. 187, 7569-7578
   Abstract »    Full Text »    PDF »
Bacillus anthracis Sortase A (SrtA) Anchors LPXTG Motif-Containing Surface Proteins to the Cell Wall Envelope.
A. H. Gaspar, L. A. Marraffini, E. M. Glass, K. L. DeBord, H. Ton-That, and O. Schneewind (2005)
J. Bacteriol. 187, 4646-4655
   Abstract »    Full Text »    PDF »
Identification and preliminary characterization of cell-wall-anchored proteins of Staphylococcus epidermidis.
M. G. Bowden, W. Chen, J. Singvall, Y. Xu, S. J. Peacock, V. Valtulina, P. Speziale, and M. Hook (2005)
Microbiology 151, 1453-1464
   Abstract »    Full Text »    PDF »
Anchor Structure of Staphylococcal Surface Proteins: V. ANCHOR STRUCTURE OF THE SORTASE B SUBSTRATE IsdC.
L. A. Marraffini and O. Schneewind (2005)
J. Biol. Chem. 280, 16263-16271
   Abstract »    Full Text »    PDF »
EsxA and EsxB are secreted by an ESAT-6-like system that is required for the pathogenesis of Staphylococcus aureus infections.
M. L. Burts, W. A. Williams, K. DeBord, and D. M. Missiakas (2005)
PNAS 102, 1169-1174
   Abstract »    Full Text »    PDF »
SdrX, a Serine-Aspartate Repeat Protein Expressed by Staphylococcus capitis with Collagen VI Binding Activity.
Y. Liu, B. Ames, E. Gorovits, B. D. Prater, P. Syribeys, J. H. Vernachio, and J. M. Patti (2004)
Infect. Immun. 72, 6237-6244
   Abstract »    Full Text »    PDF »
Characterization of the Streptococcus mutans P1 Epitope Recognized by Immunomodulatory Monoclonal Antibody 6-11A.
N. R. Rhodin, J. M. Cutalo, K. B. Tomer, W. P. McArthur, and L. J. Brady (2004)
Infect. Immun. 72, 4680-4688
   Abstract »    Full Text »    PDF »
Controlled expression of CluA in Lactococcus lactis and its role in conjugation.
R. Stentz, K. Jury, T. Eaton, M. Parker, A. Narbad, M. Gasson, and C. Shearman (2004)
Microbiology 150, 2503-2512
   Abstract »    Full Text »    PDF »
Crystal Structures of Staphylococcus aureus Sortase A and Its Substrate Complex.
Y. Zong, T. W. Bice, H. Ton-That, O. Schneewind, and S. V. L. Narayana (2004)
J. Biol. Chem. 279, 31383-31389
   Abstract »    Full Text »    PDF »
Proteomics of Protein Secretion by Bacillus subtilis: Separating the "Secrets" of the Secretome.
H. Tjalsma, H. Antelmann, J. D.H. Jongbloed, P. G. Braun, E. Darmon, R. Dorenbos, J.-Y. F. Dubois, H. Westers, G. Zanen, W. J. Quax, et al. (2004)
Microbiol. Mol. Biol. Rev. 68, 207-233
   Abstract »    Full Text »    PDF »
Sortase B, a New Class of Sortase in Listeria monocytogenes.
H. Bierne, C. Garandeau, M. G. Pucciarelli, C. Sabet, S. Newton, F. Garcia-del Portillo, P. Cossart, and A. Charbit (2004)
J. Bacteriol. 186, 1972-1982
   Abstract »    Full Text »    PDF »
Subinhibitory Concentrations of Linezolid Reduce Staphylococcus aureus Virulence Factor Expression.
K. Bernardo, N. Pakulat, S. Fleer, A. Schnaith, O. Utermohlen, O. Krut, S. Muller, and M. Kronke (2004)
Antimicrob. Agents Chemother. 48, 546-555
   Abstract »    Full Text »    PDF »
The csp operon of Streptococcus salivarius encodes two predicted cell-surface proteins, one of which, CspB, is associated with the fimbriae.
C. Levesque, C. Vadeboncoeur, and M. Frenette (2004)
Microbiology 150, 189-198
   Abstract »    Full Text »    PDF »
Structures of Streptococcus pneumoniae Hyaluronate Lyase in Complex with Chondroitin and Chondroitin Sulfate Disaccharides: INSIGHTS INTO SPECIFICITY AND MECHANISM OF ACTION.
D. J. Rigden and M. J. Jedrzejas (2003)
J. Biol. Chem. 278, 50596-50606
   Abstract »    Full Text »    PDF »
Fluorescence-Activated Cell Sorting of Specific Affibody-Displaying Staphylococci.
H. Wernerus, P. Samuelson, and S. Stahl (2003)
Appl. Envir. Microbiol. 69, 5328-5335
   Abstract »    Full Text »    PDF »
The YSIRK-G/S Motif of Staphylococcal Protein A and Its Role in Efficiency of Signal Peptide Processing.
T. Bae and O. Schneewind (2003)
J. Bacteriol. 185, 2910-2919
   Abstract »    Full Text »    PDF »
Passage of Heme-Iron Across the Envelope of Staphylococcus aureus.
S. K. Mazmanian, E. P. Skaar, A. H. Gaspar, M. Humayun, P. Gornicki, J. Jelenska, A. Joachmiak, D. M. Missiakas, and O. Schneewind (2003)
Science 299, 906-909
   Abstract »    Full Text »    PDF »
Roles of Sortase in Surface Expression of the Major Protein Adhesin P1, Saliva-Induced Aggregation and Adherence, and Cariogenicity of Streptococcus mutans.
S. F. Lee and T. L. Boran (2003)
Infect. Immun. 71, 676-681
   Abstract »    Full Text »    PDF »
Construction of a Novel Transposon Mutagenesis System Useful in the Isolation of Streptococcus parasanguis Mutants Defective in Fap1 Glycosylation.
Q. Chen, H. Wu, and P. M. Fives-Taylor (2002)
Infect. Immun. 70, 6534-6540
   Abstract »    Full Text »    PDF »
Characterization of a Unique Glycosylated Anchor Endopeptidase That Cleaves the LPXTG Sequence Motif of Cell Surface Proteins of Gram-positive Bacteria.
S. G. Lee, V. Pancholi, and V. A. Fischetti (2002)
J. Biol. Chem. 277, 46912-46922
   Abstract »    Full Text »    PDF »
Communication among Oral Bacteria.
P. E. Kolenbrander, R. N. Andersen, D. S. Blehert, P. G. Egland, J. S. Foster, and R. J. Palmer Jr. (2002)
Microbiol. Mol. Biol. Rev. 66, 486-505
   Abstract »    Full Text »    PDF »
Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus. III. LIPID II IS AN IN VIVO PEPTIDOGLYCAN SUBSTRATE FOR SORTASE-CATALYZED SURFACE PROTEIN ANCHORING.
A. M. Perry, H. Ton-That, S. K. Mazmanian, and O. Schneewind (2002)
J. Biol. Chem. 277, 16241-16248
   Abstract »    Full Text »    PDF »
rgf Encodes a Novel Two-Component Signal Transduction System of Streptococcus agalactiae.
B. Spellerberg, E. Rozdzinski, S. Martin, J. Weber-Heynemann, and R. Lutticken (2002)
Infect. Immun. 70, 2434-2440
   Abstract »    Full Text »    PDF »
The Clostridium ramosum IgA Proteinase Represents a Novel Type of Metalloendopeptidase.
K. Kosowska, J. Reinholdt, L. K. Rasmussen, A. Sabat, J. Potempa, M. Kilian, and K. Poulsen (2002)
J. Biol. Chem. 277, 11987-11994
   Abstract »    Full Text »    PDF »
The Sortase SrtA of Listeria monocytogenes Is Involved in Processing of Internalin and in Virulence.
C. Garandeau, H. Reglier-Poupet, I. Dubail, J.-L. Beretti, P. Berche, and A. Charbit (2002)
Infect. Immun. 70, 1382-1390
   Abstract »    Full Text »    PDF »
Characterization of Streptococcus suis Genes Encoding Proteins Homologous to Sortase of Gram-Positive Bacteria.
M. Osaki, D. Takamatsu, Y. Shimoji, and T. Sekizaki (2002)
J. Bacteriol. 184, 971-982
   Abstract »    Full Text »    PDF »
A high-molecular-mass cell-surface protein from Lactobacillus reuteri 1063 adheres to mucus components.
S. Roos and H. Jonsson (2002)
Microbiology 148, 433-442
   Abstract »    Full Text »    PDF »
Identification and Characterization of a Novel 38.5-Kilodalton Cell Surface Protein of Staphylococcus aureus with Extended-Spectrum Binding Activity for Extracellular Matrix and Plasma Proteins.
M. Hussain, K. Becker, C. von Eiff, J. Schrenzel, G. Peters, and M. Herrmann (2001)
J. Bacteriol. 183, 6778-6786
   Abstract »    Full Text »    PDF »
Generation of Metal-Binding Staphylococci through Surface Display of Combinatorially Engineered Cellulose-Binding Domains.
H. Wernerus, J. Lehtio, T. Teeri, P.-A. Nygren, and S. Stahl (2001)
Appl. Envir. Microbiol. 67, 4678-4684
   Abstract »    Full Text »
Decreased Amounts of Cell Wall-Associated Protein A and Fibronectin-Binding Proteins in Staphylococcus aureus sarA Mutants due to Up-Regulation of Extracellular Proteases.
A. Karlsson, P. Saravia-Otten, K. Tegmark, E. Morfeldt, and S. Arvidson (2001)
Infect. Immun. 69, 4742-4748
   Abstract »    Full Text »    PDF »
Protein secretion and the pathogenesis of bacterial infections.
V. T. Lee and O. Schneewind (2001)
Genes & Dev. 15, 1725-1752
   Full Text »    PDF »
Listeria Pathogenesis and Molecular Virulence Determinants.
J. A. Vazquez-Boland, M. Kuhn, P. Berche, T. Chakraborty, G. Dominguez-Bernal, W. Goebel, B. Gonzalez-Zorn, J. Wehland, and J. Kreft (2001)
Clin. Microbiol. Rev. 14, 584-640
   Abstract »    Full Text »    PDF »
Improved Pattern for Genome-Based Screening Identifies Novel Cell Wall-Attached Proteins in Gram-Positive Bacteria.
R. Janulczyk and M. Rasmussen (2001)
Infect. Immun. 69, 4019-4026
   Abstract »    Full Text »    PDF »
Pneumococcal Virulence Factors: Structure and Function.
M. J. Jedrzejas (2001)
Microbiol. Mol. Biol. Rev. 65, 187-207
   Abstract »    Full Text »    PDF »
Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcusaureus.
U. Ilangovan, H. Ton-That, J. Iwahara, O. Schneewind, and R. T. Clubb (2001)
PNAS 98, 6056-6061
   Abstract »    Full Text »    PDF »
Inactivation of the srtA Gene in Streptococcus gordonii Inhibits Cell Wall Anchoring of Surface Proteins and Decreases In Vitro and In Vivo Adhesion.
T. C. Bolken, C. A. Franke, K. F. Jones, G. O. Zeller, C. H. Jones, E. K. Dutton, and D. E. Hruby (2001)
Infect. Immun. 69, 75-80
   Abstract »    Full Text »    PDF »
Molecular Strategies for Fimbrial Expression and Assembly.
Hui Wu and P. M. Fives-Taylor (2001)
Critical Reviews in Oral Biology & Medicine 12, 101-115
   Abstract »    Full Text »    PDF »
Antigenicity, Expression, and Molecular Characterization of Surface-Located Pullulanase of Streptococcus pneumoniae.
R. J. M. Bongaerts, H.-P. Heinz, U. Hadding, and G. Zysk (2000)
Infect. Immun. 68, 7141-7143
   Abstract »    Full Text »    PDF »
Role of Novel Choline Binding Proteins in Virulence of Streptococcus pneumoniae.
K. K. Gosink, E. R. Mann, C. Guglielmo, E. I. Tuomanen, and H. R. Masure (2000)
Infect. Immun. 68, 5690-5695
   Abstract »    Full Text »    PDF »
Protein A is the von Willebrand factor binding protein on Staphylococcus aureus.
J. Hartleib, N. Kohler, R. B. Dickinson, G. S. Chhatwal, J. J. Sixma, O. M. Hartford, T. J. Foster, G. Peters, B. E. Kehrel, and M. Herrmann (2000)
Blood 96, 2149-2156
   Abstract »    Full Text »    PDF »
Signal Peptide-Dependent Protein Transport in Bacillus subtilis: a Genome-Based Survey of the Secretome.
H. Tjalsma, A. Bolhuis, J. D. H. Jongbloed, S. Bron, and J. M. van Dijl (2000)
Microbiol. Mol. Biol. Rev. 64, 515-547
   Abstract »    Full Text »    PDF »
Sortase, a universal target for therapeutic agents against Gram-positive bacteria?.
P. Cossart and R. Jonquieres (2000)
PNAS 97, 5013-5015
   Full Text »    PDF »
The Fibronectin-binding MSCRAMM FnbpA of Staphylococcus aureus Is a Bifunctional Protein That Also Binds to Fibrinogen.
E. R. Wann, S. Gurusiddappa, and M. Hook (2000)
J. Biol. Chem. 275, 13863-13871
   Abstract »    Full Text »    PDF »
Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus. SORTASE CATALYZED IN VITRO TRANSPEPTIDATION REACTION USING LPXTG PEPTIDE AND NH2-GLY3 SUBSTRATES.
H. Ton-That, S. K. Mazmanian, K. F. Faull, and O. Schneewind (2000)
J. Biol. Chem. 275, 9876-9881
   Abstract »    Full Text »    PDF »
Staphylococcal Surface Display of Metal-Binding Polyhistidyl Peptides.
P. Samuelson, H. Wernérus, M. Svedberg, and S. Ståhl (2000)
Appl. Envir. Microbiol. 66, 1243-1248
   Abstract »    Full Text »
M-Like Proteins of Streptococcus dysgalactiae.
J. Vasi, L. Frykberg, L. E. Carlsson, M. Lindberg, and B. Guss (2000)
Infect. Immun. 68, 294-302
   Abstract »    Full Text »    PDF »
Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif.
H. Ton-That, G. Liu, S. K. Mazmanian, K. F. Faull, and O. Schneewind (1999)
PNAS 96, 12424-12429
   Abstract »    Full Text »    PDF »
Staphylococcal Surface Display of Immunoglobulin A (IgA)- and IgE-Specific In Vitro-Selected Binding Proteins (Affibodies) Based on Staphylococcus aureus Protein A.
E. Gunneriusson, P. Samuelson, J. Ringdahl, H. Grönlund, P.-A. Nygren, and S. Ståhl (1999)
Appl. Envir. Microbiol. 65, 4134-4140
   Abstract »    Full Text »
Matrix-binding proteins of Staphylococcus aureus: functional analysis of mutant and hybrid molecules.
O. Hartford, D. McDevitt, and T. J. Foster (1999)
Microbiology 145, 2497-2505
   Abstract »    Full Text »    PDF »
Anchor Structure of Staphylococcal Surface Proteins. IV. INHIBITORS OF THE CELL WALL SORTING REACTION.
H. Ton-That and O. Schneewind (1999)
J. Biol. Chem. 274, 24316-24320
   Abstract »    Full Text »    PDF »
The essential Staphylococcus aureus gene fmhB is involved in the first step of peptidoglycan pentaglycine interpeptide formation.
S. Rohrer, K. Ehlert, M. Tschierske, H. Labischinski, and B. Berger-Bachi (1999)
PNAS 96, 9351-9356
   Abstract »    Full Text »    PDF »
Functional Analysis of the Staphylococcus aureus Collagen Adhesin B Domain.
J. L. Snodgrass, N. Mohamed, J. M. Ross, S. Sau, C. Y. Lee, and M. S. Smeltzer (1999)
Infect. Immun. 67, 3952-3959
   Abstract »    Full Text »    PDF »
Staphylococcus aureus Sortase, an Enzyme that Anchors Surface Proteins to the Cell Wall.
S. K. Mazmanian, G. Liu, H. Ton-That, and O. Schneewind (1999)
Science 285, 760-763
   Abstract »    Full Text »
Multiple Enzymatic Activities of the Murein Hydrolase from Staphylococcal Phage phi 11. IDENTIFICATION OF A D-ALANYL-GLYCINE ENDOPEPTIDASE ACTIVITY.
W. W. Navarre, H. Ton-That, K. F. Faull, and O. Schneewind (1999)
J. Biol. Chem. 274, 15847-15856
   Abstract »    Full Text »    PDF »
Electron Microscopy Studies of Cell-Wall-Anchored Cellulose (Avicel)-Binding Protein (AbpS) from Streptomyces reticuli.
S. Walter, M. Rohde, M. Machner, and H. Schrempf (1999)
Appl. Envir. Microbiol. 65, 886-892
   Abstract »    Full Text »
Surface Proteins of Gram-Positive Bacteria and Mechanisms of Their Targeting to the Cell Wall Envelope.
W. W. Navarre and O. Schneewind (1999)
Microbiol. Mol. Biol. Rev. 63, 174-229
   Abstract »    Full Text »    PDF »
Role of C-Terminal Domains in Surface Attachment of the Fructosyltransferase of Streptococcus salivarius ATCC 25975.
C. Rathsam and N. A. Jacques (1998)
J. Bacteriol. 180, 6400-6403
   Abstract »    Full Text »
Anchor Structure of Staphylococcal Surface Proteins. II. COOH-TERMINAL STRUCTURE OF MURAMIDASE AND AMIDASE-SOLUBILIZED SURFACE PROTEIN.
W. W. Navarre, H. Ton-That, K. F. Faull, and O. Schneewind (1998)
J. Biol. Chem. 273, 29135-29142
   Abstract »    Full Text »    PDF »
Anchor Structure of Staphylococcal Surface Proteins. III. ROLE OF THE FemA, FemB, AND FemX FACTORS IN ANCHORING SURFACE PROTEINS TO THE BACTERIAL CELL WALL.
H. Ton-That, H. Labischinski, B. Berger-Bachi, and O. Schneewind (1998)
J. Biol. Chem. 273, 29143-29149
   Abstract »    Full Text »    PDF »
Influence of Lif, the Lysostaphin Immunity Factor, on Acceptors of Surface Proteins and Cell Wall Sorting Efficiency in Staphylococcus carnosus.
A. Strauss, G. Thumm, and F. Götz (1998)
J. Bacteriol. 180, 4960-4962
   Abstract »    Full Text »
A Fibrinogen-Binding Protein of Staphylococcus epidermidis.
M. Nilsson, L. Frykberg, J.-I. Flock, L. Pei, M. Lindberg, and B. Guss (1998)
Infect. Immun. 66, 2666-2673
   Abstract »    Full Text »    PDF »
Identification of a Gene Involved in Assembly of Actinomyces naeslundii T14V Type 2 Fimbriae.
M. K. Yeung, J. A. Donkersloot, J. O. Cisar, and P. A. Ragsdale (1998)
Infect. Immun. 66, 1482-1491
   Abstract »    Full Text »    PDF »
The Cell Wall-Anchored Streptomyces reticuli Avicel-Binding Protein (AbpS) and Its Gene.
S. Walter, E. Wellmann, and H. Schrempf (1998)
J. Bacteriol. 180, 1647-1654
   Abstract »    Full Text »
Introduction of the mec Element (Methicillin Resistance) into Staphylococcus aureus Alters In Vitro Functional Activities of Fibrinogen and Fibronectin Adhesins.
P. E. Vaudaux, V. Monzillo, P. Francois, D. P. Lew, T. J. Foster, and B. Berger-Bächi (1998)
Antimicrob. Agents Chemother. 42, 564-570
   Abstract »    Full Text »
The Adhesion-Associated sca Operon in Streptococcus gordonii Encodes an Inducible High-Affinity ABC Transporter for Mn2+ Uptake.
P. E. Kolenbrander, R. N. Andersen, R. A. Baker, and H. F. Jenkinson (1998)
J. Bacteriol. 180, 290-295
   Abstract »    Full Text »
Functional Display of a Heterologous Protein on the Surface of Lactococcus lactis by Means of the Cell Wall Anchor of Staphylococcus aureus Protein A.
L. Steidler, J. Viaene, W. Fiers, and E. Remaut (1998)
Appl. Envir. Microbiol. 64, 342-345
   Abstract »    Full Text »
Anchor Structure of Staphylococcal Surface Proteins. A BRANCHED PEPTIDE THAT LINKS THE CARBOXYL TERMINUS OF PROTEINS TO THE CELL WALL.
H. Ton-That, K. F. Faull, and O. Schneewind (1997)
J. Biol. Chem. 272, 22285-22292
   Abstract »    Full Text »    PDF »
Streptococcal Adhesion and Colonization.
H.F. Jenkinson and R. Lamont (1997)
Critical Reviews in Oral Biology & Medicine 8, 175-200
   Abstract »    Full Text »    PDF »
Molecular Cloning and Expression of the Gene for Elastin-binding Protein (ebpS) in Staphylococcus aureus.
P. W. Park, J. Rosenbloom, W. R. Abrams, J. Rosenbloom, and R. P. Mecham (1996)
J. Biol. Chem. 271, 15803-15809
   Abstract »    Full Text »    PDF »
A Monoclonal Antibody Enhances Ligand Binding of Fibronectin MSCRAMM (Adhesin) from Streptococcus dysgalactiae.
P. Speziale, D. Joh, L. Visai, S. Bozzini, K. House-Pompeo, M. Lindberg, and M. Höök (1996)
J. Biol. Chem. 271, 1371-1378
   Abstract »    Full Text »    PDF »
Protein SIC, a Novel Extracellular Protein of Streptococcus pyogenes Interfering with Complement Function.
P. Akesson, A. G. Sjöholm, and L. Björck (1996)
J. Biol. Chem. 271, 1081-1088
   Abstract »    Full Text »    PDF »
Identification of Residues in the Staphylococcus aureus Fibrinogen-binding MSCRAMM Clumping Factor A (ClfA) That Are Important for Ligand Binding.
O. M. Hartford, E. R. Wann, M. Hook, and T. J. Foster (2001)
J. Biol. Chem. 276, 2466-2473
   Abstract »    Full Text »    PDF »
Loss of Clumping Factor B Fibrinogen Binding Activity by Staphylococcus aureus Involves Cessation of Transcription, Shedding and Cleavage by Metalloprotease.
F. M. McAleese, E. J. Walsh, M. Sieprawska, J. Potempa, and T. J. Foster (2001)
J. Biol. Chem. 276, 29969-29978
   Abstract »    Full Text »    PDF »
Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus. CYSTEINE 184 AND HISTIDINE 120 OF SORTASE FORM A THIOLATE-IMIDAZOLIUM ION PAIR FOR CATALYSIS.
H. Ton-That, S. K. Mazmanian, L. Alksne, and O. Schneewind (2002)
J. Biol. Chem. 277, 7447-7452
   Abstract »    Full Text »    PDF »
An iron-regulated sortase anchors a class of surface protein during Staphylococcus aureus pathogenesis.
S. K. Mazmanian, H. Ton-That, K. Su, and O. Schneewind (2002)
PNAS 99, 2293-2298
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)