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Science 24 March 1995:
Vol. 267. no. 5205, pp. 1831 - 1834
DOI: 10.1126/science.7892609

Articles

Science, Vol 267, Issue 5205, 1831-1834
Copyright © 1995 by American Association for the Advancement of Science


articles

Cardiac malformation in neonatal mice lacking connexin43

AG Reaume, PA de Sousa, S Kulkarni, BL Langille, D Zhu, TC Davies, SC Juneja, GM Kidder, and J Rossant

Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.

Gap junctions are made up of connexin proteins, which comprise a multigene family in mammals. Targeted mutagenesis of connexin43 (Cx43), one of the most prevalent connexin proteins, showed that its absence was compatible with survival of mouse embryos to term, even though mutant cell lines showed reduced dye coupling in vitro. However, mutant embryos died at birth, as a result of a failure in pulmonary gas exchange caused by a swelling and blockage of the right ventricular outflow tract from the heart. This finding suggests that Cx43 plays an essential role in heart development but that there is functional compensation among connexins in other parts of the developing fetus.


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Electrical Propagation in Synthetic Ventricular Myocyte Strands From Germline Connexin43 Knockout Mice.
P. Beauchamp, C. Choby, T. Desplantez, K. de Peyer, K. Green, K. A. Yamada, R. Weingart, J. E. Saffitz, and A. G. Kleber (2004)
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Expression of Gja1 correlates with the phenotype observed in oculodentodigital syndrome/type III syndactyly.
R Richardson, D Donnai, F Meire, and M J Dixon (2004)
J. Med. Genet. 41, 60-67
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Carbenoxolone blockade of neuronal network activity in culture is not mediated by an action on gap junctions.
N Rouach, M Segal, A Koulakoff, C Giaume, and E Avignone (2003)
J. Physiol. 553, 729-745
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High Glucose Alters Connexin 43 Expression and Gap Junction Intercellular Communication Activity in Retinal Pericytes.
A.-F. Li, T. Sato, R. Haimovici, T. Okamoto, and S. Roy (2003)
Invest. Ophthalmol. Vis. Sci. 44, 5376-5382
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Array analysis of gene expression in connexin-43 null astrocytes.
D. A. Iacobas, M. Urban-Maldonado, S. Iacobas, E. Scemes, and D. C. Spray (2003)
Physiol Genomics 15, 177-190
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Requirement of Gap Junctional Intercellular Communication for Human Villous Trophoblast Differentiation.
L. Cronier, J.-L. Frendo, N. Defamie, G. Pidoux, G. Bertin, J. Guibourdenche, G. Pointis, and A. Malassine (2003)
Biol Reprod 69, 1472-1480
   Abstract »    Full Text »    PDF »
Plasma Membrane Channels Formed by Connexins: Their Regulation and Functions.
J. C. SAEZ, V. M. BERTHOUD, M. C. BRANES, A. D. MARTINEZ, and E. C. BEYER (2003)
Physiol Rev 83, 1359-1400
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Regulating Cross-Talk Between Vascular Smooth Muscle Cells.
T. N. Tulenko (2003)
Arterioscler Thromb Vasc Biol 23, 1707-1709
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Regulation of Connexin43 Gap Junctional Conductance by Ventricular Action Potentials.
X. Lin, M. Crye, and R. D. Veenstra (2003)
Circ. Res. 93 , e63-e73
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Gap Junction Communication Mediates Transforming Growth Factor-{beta} Activation and Endothelial-Induced Mural Cell Differentiation.
K. K. Hirschi, J. M. Burt, K. D. Hirschi, and C. Dai (2003)
Circ. Res. 93, 429-437
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High Incidence of Cardiac Malformations in Connexin40-Deficient Mice.
H. Gu, F. C. Smith, S. M. Taffet, and M. Delmar (2003)
Circ. Res. 93, 201-206
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Astrocytic Gap Junctions Composed of Connexin 43 Reduce Apoptotic Neuronal Damage in Cerebral Ischemia.
T. Nakase, S. Fushiki, and C. C.G. Naus (2003)
Stroke 34, 1987-1993
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Regulation of Intermuscular Electrical Coupling by the Caenorhabditis elegans Innexin inx-6.
S. Li, J. A. Dent, and R. Roy (2003)
Mol. Biol. Cell 14, 2630-2644
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Impulse Propagation in Synthetic Strands of Neonatal Cardiac Myocytes With Genetically Reduced Levels of Connexin43.
S. P. Thomas, J. P. Kucera, L. Bircher-Lehmann, Y. Rudy, J. E. Saffitz, and A. G. Kleber (2003)
Circ. Res. 92, 1209-1216
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