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Science 24 March 1995:
Vol. 267. no. 5205, pp. 1807 - 1810
DOI: 10.1126/science.7534442
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Articles

Science, Vol 267, Issue 5205, 1807-1810
Copyright © 1995 by American Association for the Advancement of Science

articles

Diverse effects of the guanine nucleotide exchange factor RCC1 on RNA transport

Y Cheng, JE Dahlberg, and E Lund

Department of Biomolecular Chemistry, University of Wisconsin School of Medicine, Madison 53706.

Transport of RNAs within nuclei and through nuclear pore complexes (NPCs) are essential, but poorly understood, steps in gene expression. In experiments with mammalian cells, RCC1, the abundant nuclear guanine nucleotide exchange factor for the guanosine triphosphatase Ran/TC4, was shown to be required for nucleocytoplasmic transport of precursors of spliceosomal small nuclear RNAs (snRNAs), intranuclear transport of U3 snRNA, and processing of ribosomal RNAs, but not for export of transfer RNAs. It is proposed that guanosine triphosphate (GTP)-bound Ran/TC4 associates with ribonucleoprotein particles (RNPs) during intranuclear movement, and that GTP hydrolysis promotes deposition of RNPs at targeted sites such as NPCs or nucleoli.


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