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Science 17 March 1995:
Vol. 267. no. 5204, pp. 1652 - 1655
DOI: 10.1126/science.7886455
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Articles

Science, Vol 267, Issue 5204, 1652-1655
Copyright © 1995 by American Association for the Advancement of Science

articles

Participation of the protein Go in multiple aspects of behavior in C. elegans

JE Mendel, HC Korswagen, KS Liu, YM Hajdu-Cronin, MI Simon, RH Plasterk, and PW Sternberg

Howard Hughes Medical Institute, California Institute of Technology, Pasadena 91125.

The goa-1 gene encoding the alpha subunit of the heterotrimeric guanosine triphosphate-binding protein (G protein) Go from Caenorhabditis elegans is expressed in most neurons, and in the muscles involved in egg laying and male mating. Reduction-of-function mutations in goa-1 caused a variety of behavioral defects including hyperactive movement, premature egg laying, and male impotence. Expression of the activated Go alpha subunit (G alpha o) in transgenic nematodes resulted in lethargic movement, delayed egg laying, and reduced mating efficiency. Induced expression of activated G alpha o in adults was sufficient to cause these phenotypes, indicating that G alpha o mediates behavior through its role in neuronal function and the functioning of specialized muscles.


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