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Science 10 March 1995:
Vol. 267. no. 5203, pp. 1510 - 1512
DOI: 10.1126/science.7878472
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Articles

Science, Vol 267, Issue 5203, 1510-1512
Copyright © 1995 by American Association for the Advancement of Science

articles

Synaptic desensitization of NMDA receptors by calcineurin

G Tong, D Shepherd, and CE Jahr

Vollum Institute, Oregon Health Sciences University, Portland 97201-3098.

Desensitization is a phenomenon that is common to many ligand-gated ion channels but has been demonstrated only rarely with physiological stimulation. Numerous studies describe desensitization of the N-methyl-D-aspartate (NMDA) subtype of glutamate receptor by exogenous agonists, but whether synaptic stimulation causes desensitization has been unknown. Synaptic stimulation of NMDA receptors on rat hippocampal neurons resulted in desensitization that was prevented by intracellular 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA), adenosine-5'-O-(3-thiotriphosphate) (ATP-gamma-S), or inhibitors of phosphatase 2B (calcineurin), but not by inhibitors of phosphatases 1 and 2A or of tyrosine phosphatases. Synaptic NMDA receptors may fluctuate between phosphorylated and dephosphorylated forms, depending on the rate of synaptic stimulation and the magnitude of the associated influx of calcium through NMDA receptors.


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J. Biol. Chem. 272, 12107-12115
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A Direct Comparison of the Single-Channel Properties of Synaptic and Extrasynaptic NMDA Receptors.
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   Abstract »    Full Text »    PDF »
Characterization of Dual Leucine Zipper-bearing Kinase, a Mixed Lineage Kinase Present in Synaptic Terminals Whose Phosphorylation State Is Regulated by Membrane Depolarization via Calcineurin.
M. Mata, S. E. Merritt, G. Fan, G. G. Yu, and L. B. Holzman (1996)
J. Biol. Chem. 271, 16888-16896
   Abstract »    Full Text »    PDF »
Calcineurin Feedback Inhibition of Agonist-evoked cAMP Formation.
F. A. Antoni, R. J. O. Barnard, M. J. Shipston, S. M. Smith, J. Simpson, and J. M. Paterson (1995)
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   Abstract »    Full Text »    PDF »


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